The expression of leptin and its receptors in pre-ovulatory human follicles

Cioffi, Joseph A.; Blerkom, Jonathan Van; Antczak, M; Shafer, Alan D.; Wittmer, Severin; Snodgrass, H. Ralph

doi:10.1093/molehr/3.6.467

Cited by 317 publications

(215 citation statements)

References 18 publications

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“…We showed that, secondary to a leptin increase, the serum L:A ratio increased with exogenous gonadotropins. Given that serum and follicular levels of leptin were tightly correlated and essentially equivalent, we concluded that intrafollicular levels were derived mostly from serum transudation, and very little if at all from ovarian production, despite the known expression of leptin and its receptor in human preovulatory follicles [7]. In the linear regression model, FF insulin predicted FF leptin and the L:A ratio independently, while it indirectly correlated with FF adiponectin via leptin.…”

Section: Discussionmentioning

confidence: 82%

Ovarian adipocytokines are associated with early in vitro human embryo development independent of the action of ovarian insulin

Ferin

Sauer

et al. 2012

J Assist Reprod Genet

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Purpose We aimed to characterize the association between levels of serum and follicular fluid (FF) adipocytokines, reflected by the leptin to adiponectin ratio (L:A ratio), and oocyte quality and in vitro embryo development in women undergoing assisted reproduction. We also aimed to assess whether follicular hormonal pathways mediate this interaction. Methods We prospectively collected FF from up to four individual preovulatory follicles (n076) and fasting sera from women (n031) without endocrinopathies undergoing in vitro fertilization (IVF) at a university-based center for assisted reproduction. Leptin, total adiponectin, insulin, insulin-like growth factor 1 (IGF-1), and ovarian steriods were measured using enzyme immunoassay. Oocyte maturity, fertilization, and embryo development were assessed. Results FF leptin was similar to serum levels while FF adiponectin was lower. FF leptin (27.10±4.05 ng/mL) and the L:A ratio (11.48E−3±2.57E−3) were related to FF insulin (R 2 00.370 and 0.419, p<0.001) but not to ovarian steroids or IGF-1, whereas FF adiponectin ( 4.22±0.52 ug/mL) correlated only with leptin (R 2 0−0.138, p00.001). Oocytes from a high FF L:A ratio environment were 81 % (RR 1.81 [95%CI 0.97-3.37]) more likely to undergo successful cleavage and 117 % (RR 2.17 [95 % CI 1.06-4.44]) more likely to obtain viable cleavage morphology compared to a low FF L:A ratio environment, even when adjusted for FF insulin, an independent predictor of cleavage. Conclusions Certain adipocytokines, particularly the L:A ratio in the FF of the preovulatory follicle, are related to successful in vitro embryo development. This action may be independent of FF insulin.

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Section: Discussionmentioning

confidence: 82%

Ovarian adipocytokines are associated with early in vitro human embryo development independent of the action of ovarian insulin

Ferin

Sauer

et al. 2012

J Assist Reprod Genet

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“…Leptin protein has been found in follicular fluid, with concentrations corresponding to those reported in serum [55]. Leptin plays a role in both follicular development, where leptin transcript has been detected at early follicular stages, whereas leptin protein appears only in mature follicles [56], and subsequent luteal function.…”

Section: Role Of Leptin In Ovary Functionmentioning

confidence: 75%

Role of leptin in female reproduction

Pérez-Pérez

Sánchez-Jiménez

Maymó

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

125

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Abstract:Reproductive function is dependent on energy resources. The role of weight, body composition, fat distribution and the effect of diet have been largely investigated in experimental female animals as well as in women. Any alteration in diet and/or weight may induce abnormalities in timing of sexual maturation and fertility. However, the cellular mechanisms involved in the fine coordination of energy balance and reproduction are largely unknown. The brain and hypothalamic structures receive endocrine and/or metabolic signals providing information on the nutritional status and the degree of fat stores. Adipose tissue acts both as a store of energy and as an active endocrine organ, secreting a large number of biologically important molecules termed adipokines. Adipokines have been shown to be involved in regulation of the reproductive functions. The first adipokine described was leptin. Extensive research over the last 10 years has shown that leptin is not only an adipose tissue-derived messenger of the amount of energy stores to the brain, but also a crucial hormone/cytokine for a number of diverse physiological processes, such as inflammation, angiogenesis, hematopoiesis, immune function, and most importantly, reproduction. Leptin plays an integral role in the normal physiology of the reproductive system with complex interactions at all levels of the hypothalamic-pituitary gonadal (HPG) axis. In addition, leptin is also produced by placenta, where it plays an important autocrine function. Observational studies have demonstrated that states of leptin excess, deficiency, or resistance can be associated with abnormal reproductive function. This review focuses on the leptin action in female reproduction.

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“…Leptin is a secreted protein produced in adipocytes; its secretion increases with food intake and decreases during starvation. Its primary site of action is the hypothalamus; however, the leptin receptor has also been indentified in rodent and human theca cells, granulosa cells, oocytes, and embryos [51,52]. In rodent models, high concentrations of leptin can impair follicle development, ovulation, and oocyte maturation [53,54].…”

Section: Oocyte Maturation and Fertilizationmentioning

confidence: 99%

The impact of obesity on egg quality

Purcell

Moley

2011

J Assist Reprod Genet

132

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Obesity in women is a concern in many countries. This causes numerous health issues; however, this review focuses on the impact of obesity on women's reproduction, and in particular the oocyte. Data from infertility clinics and experimental animal models that address the effects of obesity are presented. Bidirectional communication and metabolic support from the surrounding cumulus cells are critical for oocyte development, and the impact of obesity on these cells is also addressed. Both oocyte maturation and metabolism are impaired due to obesity, negatively impacting further development. In addition to reproductive hormones, obesity induced elevations in insulin, glucose, or free fatty acids, and changes in adipokines appear to impact the developmental competence of the oocyte. The data indicate that any one of these hormones or metabolites can impair oocyte developmental competence in vivo, and the combination of all of these factors and their interactions are the subject of ongoing investigations.

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The expression of leptin and its receptors in pre-ovulatory human follicles

Cited by 317 publications

References 18 publications

Ovarian adipocytokines are associated with early in vitro human embryo development independent of the action of ovarian insulin

Ovarian adipocytokines are associated with early in vitro human embryo development independent of the action of ovarian insulin

Role of leptin in female reproduction

The impact of obesity on egg quality

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