The Expression of Involucrin, Loricrin, and Filaggrin in Cultured Sebocytes

Lee, Weon Ju; Park, Kyung Hea; Wuk, Hyun; Sohn, Mi Yeung; Park, Kyung Duck; Lee, Seok Jong; Kim, Do Won

doi:10.5021/ad.2014.26.1.134

Cited by 16 publications

(9 citation statements)

References 6 publications

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“…Keratinocytes of the epidermis differentiate and migrate to SC which contains corneocytes. These corneocytes are enriched with NMF and IVL and FLG, the representative genes of keratinocyte differentiation markers [ 25 ]. Our studies explored that AFWE promotes degradation of PFLG to FLG to produce NMF significantly higher in comparison to control and positive control groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aloe vera Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies

Sultana

Park

Shin³

et al. 2021

Molecules

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Skin moisturization is very crucial for maintaining the flexibility, viscoelasticity, and differentiation of the epidermis and its deprivation causes several diseases from dry skin to dermatitis. Aloe vera, a miracle plant having diverse medicinal properties including skin moisturization effects. This study investigated for the first time the molecular mechanism targeting skin moisturization effects of the Aloe vera flower and its major active constituent. By treating human epidermal keratinocytes (HaCaT cells) with Aloe vera flower water extract (AFWE), we found that AFWE upregulated epidermal involucrin by activating the expression of protein kinase C, p38, and ERK 1/2. Additionally, it modulated filaggrin, increased aquaporin expression, and hyaluronan synthesis via a balanced regulation of HAS1 and HYAL1 protein. Similarly, it was able to protect UVB-induced photodamage. Western blot analysis, ELISA, and qRT- PCR were performed to evaluate various epidermal differentiation markers and moisturization-related factors on human epidermal keratinocytes (HaCaT cells). TLC and HPLC were used to detect and analyze the chemical constituents. Among them, we found that an active component of Aloe vera flower, isoorientin (IO) has a high binding affinity to all of its targeted proteins such as involucrin, PKC, P38, etc. through molecular docking assay. This study indicated that the Aloe vera flower and its active constituent, IO can be used as a prominent ingredient to enhance skin barrier function and improve its related pathologies.

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Section: Discussionmentioning

confidence: 99%

Effects of Aloe vera Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies

Sultana

Park

Shin³

et al. 2021

Molecules

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“…Additionally, a cornified cell envelope is composed of filaggrin, loricrin, and involucrin, and it contains differentiated epidermal keratinocytes and corneocytes. One of the most important roles of the cornified cell envelope is maintaining skin barrier function and moisture [27]. In this study, we found that HU-018 can reduce skin damage, decrease TEWL and EI, and increase the level of skin hydration in an animal model of photoaging.…”

Section: Discussionmentioning

confidence: 58%

Skin Hydration Effects of Scale‐Up Fermented Cyclopia intermedia against Ultraviolet B‐Induced Damage in Keratinocyte Cells and Hairless Mice

Yeon

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Photoaging occurs by chronic skin exposure to the sun and ultraviolet irradiation and leads to skin aging accompanied by a lack of skin hydration. We previously demonstrated the photoprotective effect of fermented Cyclopia intermedia (honeybush) extract on the skin. In this study, we evaluated the skin hydration effects of scaled-up fermented honeybush extract (HU-018) against ultraviolet B (UVB) radiation in HaCaT immortalized human keratinocytes and hairless mice. Pretreating HaCaT cells with HU-018 attenuated the decreased hyaluronic acid (HA) levels and mRNA expression of genes encoding involucrin, filaggrin, and loricrin by UVB irradiation. HU-018 treatment also ameliorated the decreased stratum corneum (SC) hydration and the increased levels of transepidermal water loss (TEWL) and erythema index (EI) in hairless mice after UVB exposure. Microarray analysis revealed changes in gene expression patterns of hyaluronan synthase 2 (Has2), transforming growth factor-beta 3 (TGF-β3), and elastin induced by HU-018 in UVB-irradiated mice. Consistently, the mRNA expression of Has2, TGF-β3, and elastin was increased by HU-018 treatment. Moreover, HU-018 restored the increased epidermal thickness and collagen disorganization in skin tissue of UVB-irradiated mice. HU-018 treatment also decreased matrix metalloproteinase-1 (MMP-1) expression and increased procollagen type-1, elastin, and TGF-β1 expression. In conclusion, we found that HU-018 promoted skin hydration processes in UVB-irradiated keratinocytes and hairless mice by modulating involucrin, filaggrin, loricrin, and HA expression and ameliorating visible signs of photoaging. Thus, HU-018 may be a good skin hydration agent for skin care.

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“…Specific keratins KRT1 and KRT10 were much more expressed in response to Lythrum salicaria extract. These keratins are markers of supra-basal layers and clearly indicate that epidermal cells are developing towards a terminal differentiation state [20]. The high levels of filaggrin and loricrin in skins treated with the extract participated to the resistance of the cornified envelope.…”

Section: Levels Of Expression Of Genes Implicated In Differentiationmentioning

confidence: 98%

“…As shown in Figure 2, the incubation of the cells with extract induced a strong increase in the mRNA of differentiation markers, mainly TGK, keratin 1 (KRT1) and KRT10, and to a lesser extent, filaggrin, loricrin and involucrin (from 400% to 6000% of the untreated control depending of the marker). Looking at the presence of various proteins, such as involucrin, loricrin, and filaggrin gives indications of fully differentiated epidermal keratinocytes and corneocytes [20]. These structural proteins help to maintain the cutaneous barrier.…”

Section: Levels Of Expression Of Genes Implicated In Differentiationmentioning

confidence: 99%

New Biological Activities of Lythrum salicaria L.: Effects on Keratinocytes, Reconstructed Epidermis and Reconstructed Skins, Applications in Dermo-Cosmetic Sciences

Jouravel

Guenin²,

Bernard³

et al. 2017

Cosmetics

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Abstract:The perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine. Purple loosestrife is known for its ability to treat internal disorders, such as gastrointestinal issues or hemorrhages. Our objective was to take another look on this natural source of ellagitannins in terms of biological activities. Exploration of the phytochemical content of an extract of aerial parts of Lythrum salicaria L. was completed before initiating research on its biological effects towards keratinocytes, reconstructed epidermis, and skins. The potential of the natural compounds were evaluated by topical treatment of reconstructed tissues. The extract and one of its major compounds were able to act as pro-differentiating and protecting agents towards skin cells by stimulating the expressions of markers taking part in the structure of epidermis and dermis. Also, the extract showed beneficial effects on the global morphology of the skin. Thus, Lythrum salicaria L. constitutes a new natural source for the development of active ingredients for the dermo-cosmetic field.

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The Expression of Involucrin, Loricrin, and Filaggrin in Cultured Sebocytes

Cited by 16 publications

References 6 publications

Effects of Aloe vera Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies

Effects of Aloe vera Flower Extract and Its Active Constituent Isoorientin on Skin Moisturization via Regulating Involucrin Expression: In Vitro and Molecular Docking Studies

Skin Hydration Effects of Scale‐Up Fermented Cyclopia intermedia against Ultraviolet B‐Induced Damage in Keratinocyte Cells and Hairless Mice

New Biological Activities of Lythrum salicaria L.: Effects on Keratinocytes, Reconstructed Epidermis and Reconstructed Skins, Applications in Dermo-Cosmetic Sciences

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