The expression of immunoglobulin determinants on the surface of antigen‐binding lymphoid cells in mice. II. Allotypes of the lg‐1 locus

Abstract

Inhibition of rosette-forming cells by anti-immunoglobulin sera has been used as a model system to investigate the phenotypic expression of IgCza allotypes on the surface of mouse spleen cells reactive against sheep erythrocyte antigens.The evidence presented suggests that reactive cells from mice heterozygous at the Ig-1 locus may not be restricted t o the expression of the product of only one parental allele on their surface. Control studies with mixtures of cells from mice compatible with respect t o H-2 antigens but differing at the Ig-1 locus suggest that this finding is not the result of a passive absorption phenomenon.This apparent lack of allellic exclusion was demonstrated with cells harvested during the early part of the response t o a primary injection of antigen. Reactive cells later in the immune response appeared t o be restricted t o the expression of one parental allotype.

Separation of Cells According to Surface Antigens by the Use of Antibody‐Coated Columns. Fractionation of Cells Carrying Immunoglobulins and Blood Group Antigen

“…The question whether mouse B lymphoid cells carry some or only one 7S immunoglobulin class on the outer surface is still very controversial (9). The present results using gamma-2a plus anti-gamma-2a specific serum, as presented in Table II, would support the view that memory cells of B type only carry the heavy chain antigens of one immunoglobulin on the surface.…”

Genetics, Cellular Expression and Function of IgD and IgM Receptors

“…While allelic exclusion has been amply demonstrated for antibody forming cells (Pemis et al 1965, Cebra et al 1966, the question of whether or not resting B cells exhibit allelic exclusion has been controversial (Greaves 1971, Wolf et al 1971, Anderson 1972, Wamer 1974, Linthicum & Sell 1976, Sell 1977. It must be emphasized that all the possible sources of technical error (cytophilic antibodies, inadequate specificity of antisera, binding of aggregates and complexes to Fc receptors, and natural antimembrane antibodies) will cause errors in the direction of a failure to demonstrate allelic exclusion.…”

A Speculative Comparison of Allotypic Suppression and Allotypic Blast Transformation: Control of Expression of T and B Cell Immunoglobulin Allotypic Markers by a B Cell Product

“…Dubiski (1967b) postulated that double-expressing reactive cells might become restricted upon reaction with antigen. However, both Greaves (1971) and Anderson (1972) found double allotype-expressing antibody-forming cells and concluded that restriction must occur at some time during differentiation of the B-cell line to well-differentiated plasma cells. It is possible that even differentiated plasma eells may have both alleles active but at such an imbalanee that only one is detected by techniques such as immunofiuorescence.…”