The expression of HtrA2 and its diagnostic value in patients with hepatocellular carcinoma

Abstract

The purpose of this study was to detect the expression of high-temperature requirement A2 (HtrA2) and its diagnostic value in the patients with hepatocellular carcinoma (HCC).The relative serum HtrA2 expression at mRNA and protein level was severally detected by quantitative real-time polymerase chain reaction and western blot analysis in 198 HCC patients and 48 healthy controls. And its association with clinicopathological features was analyzed by chi-square test. The diagnostic value of HtrA2 expression was estimated by establishing a receiver operating characteristic (ROC) curve.Serum HtrA2 was significantly higher in patients with HCC than that in healthy controls both at mRNA and protein levels (P < .05 for both). In addition, the high HtrA2 expression was associated with large tumor size and advanced clinical stage. Furthermore, the value of the area under the ROC curve was 0.808 corresponding with a sensitivity of 65.2% and a specificity of 89.6%, revealed that HtrA2 might be a diagnostic biomarker in HCC.HtrA2 is upregulated and considered to be a potential biomarker for the diagnosis of patients with HCC.

Mitochondrial quality control proteases and their modulation for cancer therapy

“…The investigators indicated that HTRA2, served as an oncogene, is significantly correlated with tumor size and clinical stage. That is, patients with high expression of HTRA2 are more likely to develop large tumors and progress to clinically advanced stages, which is consistent with the ROC curves that suggest HTRA2 is highly diagnostic for cancer 118 . By contrast, lower expression of HTRA2 in some cancer tissues than their normal counterparts suggests the cancer‐suppressive effect of HTRA2 (Figure 6).…”

“…That is, patients with high expression of HTRA2 are more likely to develop large tumors and progress to clinically advanced stages, which is consistent with the ROC curves that suggest HTRA2 is highly diagnostic for cancer. 118 By contrast, lower expression of HTRA2 in some cancer tissues than their normal counterparts suggests the cancer-suppressive effect of HTRA2 (Figure 6). The analysis for 65 colorectal cancer patients conducted by Dorota et al verified that HTRA2 mRNA and protein levels are obviously lower in cancerous tissues than in normal colorectal mucosa, especially in patients with metastatic colorectal cancer.…”

HtrA2 Independently Predicts Poor Prognosis and Correlates with Immune Cell Infiltration in Hepatocellular Carcinoma

“…According to the results of previous studies, higher expression of HtrA2 had been found in malignant thyroid tumors, gastric cancer, and prostate cancer, and it was predictive of poor patient outcomes [ 27 – 30 ]. However, the expression of HtrA2 was found to be very low, which predicted poor outcomes in non-small-cell lung cancer and ovarian cancer [ 31 , 32 ].…”

Role of High-temperature Requirement Serine Protease A 2 in Rheumatoid Inflammation

“…HtrA2 is localized to the mitochondrial intermembrane space and can in uence the physiology of mitochondrial homeostasis [8, 9,17]. Its aberrant expression has been linked to a variety of illnesses, including ovarian cancer, endometrial cancer, as well as neurological disorders [18][19][20][21].…”

“…It is an apoptosis-inducing protein that is released after an apoptotic stimulus from the mitochondrial intermembrane space into the cytosol [8]. HtrA2 has roles in the progression of neurodegenerative diseases, prostate cancer, and hepatocellular carcinoma [8,9]. However, HtrA2 controls in ammation and immune response remains unknown.…”