The Expression of Histone Demethylase JMJD1A in Renal Cell Carcinoma

Guo, Xiaoqiang; Shi, Mengsha; Sun, Lai Guang; Wang, Y; Gui, Yaoting; Cai, Zhiming; Duan, Xingguang

doi:10.4149/neo_2011_02_153

Cited by 50 publications

(32 citation statements)

References 25 publications

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“…1(D)]. These results suggested that JMJD1A is also a hypoxia-response gene in RCC cells, which is consistent with our previous research in mimicking hypoxia [13].…”

Section: Resultssupporting

confidence: 81%

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Ascorbate antagonizes nickel ion to regulate JMJD1A expression in kidney cancer cells

Guo¹,

Lu²,

Wang³

et al. 2012

ABBS

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Abnormal expression of histone demethylase Jumonji domain-containing protein 1A (JMJD1A) is associated with many kinds of cancers. JMJD1A is also a hypoxic response gene and its expression is regulated by hypoxia-inducible factor-1a . In this study, we determined the role of JMJD1A in development and hypoxia pathway. We also measured the expression of JMJD1A and two hypoxia factors glucose transporter 1 (GLUT1) and vascular endothelial growth factor (VEGF) in 786-0 and HEK293 cells treated with different concentrations of NiCl 2 (2.5 -100 mM) for 24 h, and found that JMJD1A mRNA and protein were up-regulated with increased concentrations of NiCl 2 . We then observed that ascorbate could retard the up-regulated effect of NiCl 2 -induced JMJD1A expression in a dose-dependent manner through decreasing the stability of HIF-1a protein.Immunohistochemical analysis further demonstrated ascorbate antagonized Ni 21 -induced up-regulation of JMJD1A expression in 786-0, HEK293, and OS-RC-2 cells. These findings suggest that both Ni 21 and ascorbate can regulate the expression of histone demethylase JMJD1A, which is important for cancer development or inhibition.

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“…1(D)]. These results suggested that JMJD1A is also a hypoxia-response gene in RCC cells, which is consistent with our previous research in mimicking hypoxia [13].…”

Section: Resultssupporting

confidence: 81%

“…Several researches have indicated that abnormal expression of JMJD1A exists in many kinds of cancers, including colorectal, prostate, and kidney cancers [10][11][12]. Our previous experiment also confirmed that high expression of JMJD1A is associated with renal cell carcinoma (RCC) [13].…”

Section: Introductionsupporting

confidence: 66%

Ascorbate antagonizes nickel ion to regulate JMJD1A expression in kidney cancer cells

Guo¹,

Lu²,

Wang³

et al. 2012

ABBS

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“…For example, KDM3A expression was found to be elevated in certain types of cancer (64,65). In one particular study, immunohistochemical analysis revealed higher levels of KDM3A near the vessels of renal cell carcinomas, implicating a possible role in regulation of VEGF (65).…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Modifies Global Histone Methylation by Inhibiting Jumonji C Domain-containing Demethylases

Hickok

Vasudevan

Antholine

et al. 2013

Journal of Biological Chemistry

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Background:The methylation status of histone tails is a balance between methylation and demethylation. Results: Nitric oxide inhibits lysine demethylase 3A and alters cellular histone methylation patterns. Conclusion: Nitric oxide can significantly modify the epigenetic landscape. Significance: These results establish nitric oxide as a physiological epigenetic regulator acting through a nonclassical cell signaling mechanism.

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“…Based on the facts that cancer cells showed elevated expression levels of KDM3A, which lead to upregulation of cell proliferation (64)(65)(66), and KDM3A is a critical epigenetic modifier for robust gene expression under conditions of hypoxia (34,62), inhibition of KDM3A may have cancer therapeutic potential for KSHV-associated malignancies via inhibition of cell growth as well as viral replication.…”

Section: Discussionmentioning

confidence: 99%

Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Latency-Associated Nuclear Antigen Regulates the KSHV Epigenome by Association with the Histone Demethylase KDM3A

Kim

Huerta

Izumiya

et al. 2013

J Virol

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Kaposi's sarcoma-associated herpesvirus (KSHV) latent genomes are tethered to host histones to form a minichromosome also known as an "episome." Histones, which are core components of chromatin, are heavily modified by various histone-targeting enzymes. Posttranslational modifications of histones significantly influence accessibility of transcriptional factors and thus have profound effects on gene expression. Recent studies showed that epigenetic marks on the KSHV episome are well organized, exemplified by the absence of histone H3 lysine 9 (H3K9) methylation, a heterochromatic histone mark, from immediate early and latent gene promoters in naturally infected cells. The present study revealed a mechanistic insight into KSHV epigenome regulation via a complex consisting of LANA and the H3K9me1/2 histone demethylase JMJD1A/KDM3A. This complex was isolated from HeLa cell nuclear extracts stably expressing LANA and was verified by coimmunoprecipitation analyses and with purified proteins. LANA recruitment sites on the KSHV genome inversely correlated with H3K9me2 histone marks in naturally infected cells, and methylation of H3K9 significantly inhibited LANA binding to the histone H3 tail. Chromatin immunoprecipitation coupled with KSHV tiling arrays identified the recruitment sites of the complex, while depletion of LANA expression or overexpression of a KDM3A binding-deficient mutant decreased KDM3A recruitment to the KSHV genome. Finally, ablation of KDM3A expression from latently KSHV-infected cells significantly inhibited KSHV gene expression, leading to decreased KSHV replication during reactivation. Taken together, our results suggest that LANA may play a role in regulation of epigenetic marks on the KSHV genome, which is in part through association with the histone demethylase KDM3A.

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The Expression of Histone Demethylase JMJD1A in Renal Cell Carcinoma

Cited by 50 publications

References 25 publications

Ascorbate antagonizes nickel ion to regulate JMJD1A expression in kidney cancer cells

Ascorbate antagonizes nickel ion to regulate JMJD1A expression in kidney cancer cells

Nitric Oxide Modifies Global Histone Methylation by Inhibiting Jumonji C Domain-containing Demethylases

Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Latency-Associated Nuclear Antigen Regulates the KSHV Epigenome by Association with the Histone Demethylase KDM3A

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