The expression of genes related to adipocyte differentiation in pigs1

Abstract

The purpose of this study was to detect differential expression of genes related to adipocyte differentiation in pigs by suppression subtractive hybridization. Adipocytes and stromal vascular cells (a fraction containing preadipocytes) from pig adipose tissue were isolated for mRNA extraction. The cDNA from preadipocytes was subtracted from the cDNA from adipocytes. The subtracted gene fragments were cloned into pGEM-T Easy TA cloning vector. We selected 384 clones for gene sequence determination and for further analysis. These genes were subjected to a differential screening procedure to confirm the differential expression of genes between the 2 cell types. We found that at least 36 genes were highly expressed in the adipocytes compared with preadipocytes. Among these, 6 genes including 2 novel genes with the greatest differences were selected and confirmed by Northern analysis. We found that angiotensin I-converting enzyme (ACE), ataxia-telangiectasia mutated protein (ATM), calpain 1, and stearoyl coenzyme A desaturase 1 (SCD1) were highly expressed in adipocytes compared with preadipocytes (P < 0.05). The relative mRNA abundance of ACE, ATM, calpain 1, SCD1, and 2 novel genes discovered in the current study was increased at the later stages of adipocyte differentiation (P < 0.05). The results confirmed that the genes involved in lipid metabolism and adipocyte differentiation were highly expressed in porcine adipocytes. However, further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study.

Porcine peroxisome proliferator-activated receptor γ induces transdifferentiation of myocytes into adipocytes1

“…Rosi = 1 M of rosiglitazone treatment. et al, 2004;Wang et al, 2006). The densitometric value for an individual transcript in a sample lane was normalized to the densitometric value for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the same lane.…”

Angiotensin-Converting Enzyme (ACE) Inhibitors Modulate Cellular Retinol-Binding Protein 1 and Adiponectin Expression in Adipocytes via the ACE-Dependent Signaling Cascade

“…The results of the present investigation indicate that ACE inhibitors increase the expression of CRBP1. However, because this protein would be expected to have greater effects on adipocytes, which in addition to being involved in retinoid storage and metabolism express high levels of ACE (Wang et al, 2006), the majority of our study investigated its effects either in adipocytes or in vivo. Studies in rats indicate that 15 to 20% of the total retinoid content in the body can be stored in adipose tissue (Tsutsumi et al, 1992), and CRBP1 expression in adipocytes is regulated by retinoic acid, dexamethasone, and tri-iodothyronine (Okuno et al, 1995) , an unrelated control siRNA (each 50 pmol) or transfection reagent alone (without siRNA) on the expression of a histidinetagged CRBP1 in human embryonic kidney 293 cells.…”

Differential Gene Expression Between the Porcine Morula and Blastocyst

“…A transgenic mouse lacking a functional ATM gene has early embryonic lethality (Huber et al 2004). The porcine ATM gene was cloned (Wang et al 2006) and is expressed ubiquitously in pig adult tissues with the greatest expression in tissues with the most proliferation (Rogatcheva et al 2007). Perhaps, the rapid increase in blastocyst cell number required tighter control over DNA replication and the cell cycle, thus requiring increased ATM.…”