The expression of early light‐inducible proteins (ELIPs) under high‐light stress as defense marker in Northern‐ and Southern European cultivars of barley (<i>Hordeum vulgare</i>)

Bei‐Paraskevopoulou, Thalia; Kloppstech, Klaus

doi:10.1034/j.1399-3054.1999.106115.x

Cited by 10 publications

(8 citation statements)

References 34 publications

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“…Barley cultivars bred for growth in northern Europe have been shown to accumulate more ELIPs than cultivars grown in southern Europe (34). This observation is consistent with our results showing a link between tolerance to chilling-induced photooxidation and ELIPs.…”

Section: Resultssupporting

confidence: 92%

Early light-induced proteins protect Arabidopsis from photooxidative stress

Hutin

Nussaume

Moise

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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The early light-induced proteins (ELIPs) belong to the multigenic family of light-harvesting complexes, which bind chlorophyll and absorb solar energy in green plants. ELIPs accumulate transiently in plants exposed to high light intensities. By using an Arabidopsis thaliana mutant (chaos) affected in the posttranslational targeting of light-harvesting complex-type proteins to the thylakoids, we succeeded in suppressing the rapid accumulation of ELIPs during high-light stress, resulting in leaf bleaching and extensive photooxidative damage. Constitutive expression of ELIP genes in chaos before light stress resulted in ELIP accumulation and restored the phototolerance of the plants to the wild-type level. Free chlorophyll, a generator of singlet oxygen in the light, was detected by chlorophyll fluorescence lifetime measurements in chaos leaves before the symptoms of oxidative stress appeared. Our findings indicate that ELIPs fulfill a photoprotective function that could involve either the binding of chlorophylls released during turnover of pigment-binding proteins or the stabilization of the proper assembly of those proteins during high-light stress.L ight is essential for plants through photosynthetic carbon assimilation. However, when absorbed light exceeds the photosynthetic capacities, reactive O 2 species are generated in the chloroplasts, causing oxidative damage to proteins, lipids, and photosynthetic pigments (1, 2). This effect is amplified by environmental stresses such as low temperature or drought, for example, that inhibit the photosynthetic activity, leading to strong yield reduction in crops. In green plants, solar energy is collected by chlorophyll-and carotenoid-binding lightharvesting complexes (LHCs), which are encoded by a multigene family of LHC genes. The expression of these genes is tightly regulated by light (2-4). High light intensities inhibit transcription of LHC genes and activate synthesis of the early lightinduced proteins (ELIPs), a class of proteins structurally related to the LHCs (5). The ELIPs are predicted to have three transmembrane helices, and they have sequence similarity to the LHCs in the central pair of helices (6, 7). The similarity is not only at the sequence level, however, because both LHCs and ELIPs bind chlorophyll and carotenoids (8). The ELIPs differ from the LHCs by their transient expression under high-light stress (5). Recently, a number of ELIP-type polypeptides, containing LHC motifs and inducible by high light, have been discovered in vascular plants: the one-helix high-light-induced proteins (9) and the two-helix stress-enhanced proteins (10).The physiological role of the ELIPs in vascular plants has not yet been elucidated, although there have been several suggestions (11)(12)(13)(14). The induction of ELIPs by high light intensities suggests a role in the acclimation to light stress rather than a light-harvesting function, but this has not yet been demonstrated. ELIP antisense transgenic tobacco plants did not exhibit any phenotype of sensitivity to high ...

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Section: Resultssupporting

confidence: 92%

Early light-induced proteins protect Arabidopsis from photooxidative stress

Hutin

Nussaume

Moise

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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294

250

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“…The mRNA level of ELIP increases markedly under cold stress in the tea plant [44] and there is a positive correlation between ELIP accumulation and tolerance to chilling-induced photooxidation in barley [45]. In the present investigation, ELIP was up-regulated in the albinistic stage of White leaf No.…”

Section: Discussionsupporting

confidence: 48%

“…Early light-inducible protein (ELIP), a member of the chlorophyll a / b binding protein family, has been implicated in the assembly or repair of the photosynthetic machinery during early chloroplast development [ 40 ] and is induced by many abiotic stresses [ 41 - 43 ]. The mRNA level of ELIP increases markedly under cold stress in the tea plant [ 44 ] and there is a positive correlation between ELIP accumulation and tolerance to chilling-induced photooxidation in barley [ 45 ]. In the present investigation, ELIP was up-regulated in the albinistic stage of White leaf No.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar

et al. 2011

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BackgroundWhite leaf No.1 is a typical albino tea cultivar grown in China and it has received increased attention in recent years due to the fact that white leaves containing a high level of amino acids, which are very important components affecting the quality of tea drink. According to the color of its leaves, the development of this tea cultivar is divided into three stages: the pre-albinistic stage, the albinistic stage and the regreening stage. To understand the intricate mechanism of periodic albinism, a comparative proteomic approach based on two-dimensional electrophoresis (2-DE) and mass spectrometry was adopted first time to identify proteins that changed in abundance during the three developmental periods.ResultsThe 2-DE results showed that the expression level of 61 protein spots varied markedly during the three developmental stages. To analyze the functions of the significantly differentially expressed protein spots, 30 spots were excised from gels and analyzed by matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry. Of these, 26 spots were successfully identified. All identified protein spots were involved in metabolism of carbon, nitrogen and sulfur, photosynthesis, protein processing, stress defense and RNA processing, indicating these physiological processes may play crucial roles in the periodic albinism. Quantitative real-time RT-PCR analysis was used to assess the transcriptional level of differentially expressed proteins. In addition, the ultrastructural studies revealed that the etioplast-chloroplast transition in the leaf cell of White leaf No. 1 was inhibited and the grana in the chloroplast was destroyed at the albinistic stage.ConclusionsIn this work, the proteomic analysis revealed that some proteins may have important roles in the molecular events involved in periodic albinism of White leaf No. 1 and identificated many attractive candidates for further investigation. In addition, the ultrastructural studies revealed that the change in leaf color of White leaf No. 1 might be a consequence of suppression of the etioplast-chloroplast transition and damage to grana in the chloroplast induced by temperature. These results provide much useful information to improve our understanding of the mechanism of albinism in the albino tea cultivar.

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“…A functional interaction between zeaxanthin and ELIPs (or its algal homolog Cbr) has been proposed (Levy et al 1993;Braun et al 1996;MontaneÂ et al 1997;Krol et al 1999). The protective function of ELIPs in high light is supported by the ®nding that, in barley, the ELIP level is well correlated with the capacity for PSII activity restoration after a photoinhibitory light treatment (Bei-Paraskevopoulou and Kloppstech 1999).…”

Section: Fig 10mentioning

confidence: 96%

The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsis npq and tt mutants

Havaux

Kloppstech

2001

Planta

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The npq1 mutant of Arabidopsis thaliana (L.) Heynh. has no xanthophyll cycle due to a lack of functional violaxanthin de-epoxidase. Short-term exposure (<2 days) of detached leaves or whole plants to the combination of high photon flux density (1,000 micromol m(-2) s(-1)) and low temperature (10 degrees C) resulted in PSII photoinhibition which was more acute in npq1 than in the wild type. This increased photosensitivity of npql at chilling temperature was attributable to the inhibition of nonphotochemical energy quenching (NPQ) and not to the absence of zeaxanthin itself. In contrast to PSII, PSI was found to be phototolerant to chilling stress in the light in both genotypes. In the long term (10-12 days), PSII activity recovered in both npql and wild type, indicating that A. thaliana is able to acclimate to chilling stress in the light independently of the xanthophyll cycle. In npql, photoacclimation involved a substantial reduction of the light-harvesting pigment antenna of PSII and an improvement of photosynthetic electron transport. Chilling stress also induced synthesis of early light-inducedproteins (ELIPs) which, in the long term, disappeared in npql and remained stable in the wild type. In both genotypes, photoacclimation at low temperature induced the accumulation of various antioxidants including carotenoids (except beta-carotene), vitamin E (alpha- and -gamma-tocopherol) and non-photosynthetic pigments (anthocyanins and other flavonoids). Analysis of flavonoid-deficient tt mutants revealed that UV/blue-light-absorbing flavonols have a strong protective function against excess visible radiations. In contrast to the defect in npq1, the absence of flavonoids could not be overcome in the long term by compensatory mechanisms, leading to extensive photooxidative and photoinhibitory damage to the chloroplasts. Depth profiling of the leaf pigments by phase-resolved photoacoustic spectroscopy showed that the flavonoid-related photoprotection was due to light trapping, which decreased chlorophyll excitation by blue light. In contrast to flavonoids, the xanthophyll cycle and the associated NPQ seem to be mainly relevant to the protection of photosynthesis against sudden increases in light intensity.

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The expression of early light‐inducible proteins (ELIPs) under high‐light stress as defense marker in Northern‐ and Southern European cultivars of barley (Hordeum vulgare)

Cited by 10 publications

References 34 publications

Early light-induced proteins protect Arabidopsis from photooxidative stress

Early light-induced proteins protect Arabidopsis from photooxidative stress

Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar

The protective functions of carotenoid and flavonoid pigments against excess visible radiation at chilling temperature investigated in Arabidopsis npq and tt mutants

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