The expression of CDK1 is associated with proliferation and can be a prognostic factor in epithelial ovarian cancer

Xi, Qinghua; Huang, Menghui; Wang, Yingying; Zhong, Jun; Liu, Rong; Xu, Guanghui; Jiang, Lifei; Wang, Juan; Fang, Zheng; Yang, Shuyun

doi:10.1007/s13277-015-3141-8

Cited by 52 publications

(43 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CDK1, a cell cycle regulator CDK family member, is the key molecule of the ATM-chk2-CDC25-CCNB1/CDK1 pathway that controls the cell cycle [24,25]. Xi et al showed that CDK1 expression is associated with ovarian cancer cell proliferation and can serve as an independent prognostic factor [26]. CCND1 stimulates G1 progression in responding to growth factor stimulation [27]; miR-211 down-regulation results in CCND1 and CDK6 overexpression, which increases the proliferative ability of EOC cells [28].SMARCD1, whose high expression predicts poorer prognosis in gastric cancer, functions as an oncogene by promoting gastric cancer cell proliferation, migration and invasion [29].…”

Section: Discussionmentioning

confidence: 99%

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR‐490‐3p and altering CDK1 expression

Wang

Sun

et al. 2017

J Cellular Molecular Medi

View full text Add to dashboard Cite

Recently, a large number of studies have focused on the important role of long non‐coding RNAs (lncRNAs) in metabolism and development and have found that abnormal lncRNA expression is associated with the pathogenesis and development of many diseases. The lncRNA DLEU1 is involved in many solid tumours and haematological malignancies. However, its role in epithelial ovarian carcinoma (EOC) and the associated molecular mechanisms has not been reported. In this study, quantitative reverse transcription–PCR (qRT–PCR) demonstrated higher lncRNA DLEU1 expression in EOC tissues than in normal tissues. Plasmid transfection of DLEU1 to up‐regulate its expression in the ovarian cancer cell lines A2780 and OVCAR3 increased cell proliferation, migration, and invasion, while inhibited apoptosis. Nude mouse xenograft assay demonstrated that DLEU1 overexpression promoted tumour growth in vivo. QRT–PCR showed decreased miR‐490‐3p expression, while Western blotting demonstrated increased its target genes CDK1, cyclinD1 and SMARCD1, as well as matrix metalloproteinase‐2 (MMP2), Bcl‐xL and P70S6K protein expression, respectively. Short interfering RNA silencing of DLEU1 produced opposite results, where qRT–PCR showed increased miR‐490‐3p expression. The dual‐luciferase reporter assay revealed a direct interaction between DLEU1 and miR‐490‐3p. MiR‐490‐3p plays a tumour suppressor role in epithelial ovarian cancer by targeting CDK1 regulation and influencing SMARCD1 and cyclin D1 (CCND1) expressions. Therefore, we suggest that through interaction with miR‐490‐3p, DLEU1 may influence the expression of CDK1, CCND1 and SMARCD1 protein, subsequently promoting the development and progression of EOC.

show abstract

Section: Discussionmentioning

confidence: 99%

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR‐490‐3p and altering CDK1 expression

Wang

Sun

et al. 2017

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Smc1A (structural maintenance of chromosomes 1A) was associated with ubiquitin-mediated proteolysis [23]. CDK1 and CycB could mediate the cell division from G1 to S and G2 to M phases [24]. Rb1 (retinoblastoma-associated protein 1) and HDAC2 (histone deacetylase 2) were involved in apoptosis, S-phase, and DNA biosynthesis [25].…”

Section: Resultsmentioning

confidence: 99%

Analysis of Differentially Expressed Proteins in Mycobacterium avium-Infected Macrophages Comparing with Mycobacterium tuberculosis-Infected Macrophages

Yang

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

Mycobacterium avium (MA) belongs to the intracellular parasitic bacteria. To better understand how MA survives within macrophages and the different pathogenic mechanisms of MA and Mycobacterium tuberculosis (MTB), tandem mass tag (TMT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis have been used to determine the proteins which are differentially expressed in MA-infected and MTB-infected macrophages. 369 proteins were found to be differentially expressed in MA-infected cells but not in MTB-infected cells. By using certain bioinformatics methods, we found the 369 proteins were involved in molecular function, biological process, and cellular component including binding, catalytic activity, metabolic process, cellular process, and cell part. In addition, some identified proteins were involved in multiple signaling pathways. These results suggest that MA probably survive within macrophages by affecting the expression of some crucial proteins.

show abstract

“…Goga et al [25] reported the inhibition of CDK1 gene resulting in downregulation of surviving expression and induction of MYC-dependent apoptosis. In addition, CDK1 gene silencing was shown to be a novel effective tool for treatment of epithelial ovarian cancer [26]. The expression of CDK2 is significantly correlated with the intrinsic breast cancer subtypes [27].…”

Section: Discussionmentioning

confidence: 99%

Microarray-Based Gene Expression Analysis Identifies Potential Diagnostic and Prognostic Biomarkers for Waldenström Macroglobulinemia

Xu¹,

Yao²

2018

Acta Haematol

View full text Add to dashboard Cite

Waldenström macroglobulinemia (WM), also known as lymphoplasmacytic lymphoma, is rare but a clinicopathologically distinct B-cell malignancy. This study assessed differentially expressed genes (DEGs) to identify potential WM biomarkers and uncover the underlying the molecular mechanisms of WM progression using gene expression profiles from the Gene Expression Omnibus database. DEGs were identified using the LIMMA package and their potential functions were then analyzed by using the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses and the protein-protein interaction (PPI) network analysis by using the Search Tool for the Retrieval of Interacting Genes/Proteins database. Data showed that among 1,756 DEGs, 926 were upregulated and 830 were downregulated by comparing WM BM CD19+ with normal PB CD19+ B cell samples, whereas 241 DEGs (95 upregulated and 146 downregulated) were identified by comparing WM BM CD138+ with normal BM CD138+ plasma cell samples. The DEGs were enriched in different GO terms and pathways, including the apoptotic process, cell cycle arrest, immune response, cell adhesion, mitogen-activated protein kinase signaling pathway, toll-like receptor signaling pathway, and the gonadotropin-releasing hormone signaling pathway. Hub nodes in the PPI network included CDK1, JUN, CREBBP, EP300, CAD, CDK2, and MAPK14. Bioinformatics analysis of the GSE9656 dataset identified 7 hub genes that might play an important role in WM development and progression. Some of the candidate genes and pathways may serve as promising therapeutic targets for WM.

show abstract

The expression of CDK1 is associated with proliferation and can be a prognostic factor in epithelial ovarian cancer

Cited by 52 publications

References 20 publications

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR‐490‐3p and altering CDK1 expression

DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR‐490‐3p and altering CDK1 expression

Analysis of Differentially Expressed Proteins in Mycobacterium avium-Infected Macrophages Comparing with Mycobacterium tuberculosis-Infected Macrophages

Microarray-Based Gene Expression Analysis Identifies Potential Diagnostic and Prognostic Biomarkers for Waldenström Macroglobulinemia

Contact Info

Product

Resources

About