The Expression of Bcl-2 and BID in Gastric Cancer Cells

Gryko, Mariusz; Pryczynicz, Anna; Zaręba, Konrad; Kędra, Bogusław; Kemonä, A; Guzińska‐Ustymowicz, Katarzyna

doi:10.1155/2014/953203

Cited by 38 publications

(33 citation statements)

References 25 publications

(38 reference statements)

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“…Attempts to correlate Ki67 and BCL2 with clinicopathologic parameters have yielded contradictory results in gastric cancer. Earlier studies demonstrated that BCL2 correlates to intestinal type rather than diffuse or mixed type and plays an important role in tumor growth of the intestinal type in gastric cancer [29,30]. Müller et al [31] reported a significant relationship between tumor cell proliferation and intestinal type, but no significant correlation between Ki67 and survival.…”

Section: Discussionmentioning

confidence: 99%

A High Ki67/BCL2 Index Could Predict Lower Disease-Free and Overall Survival in Intestinal-Type Gastric Cancer

Min

Kim²,

Son³

et al. 2017

Eur Surg Res

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Background: The heterogeneity of gastric cancer makes the identification of potential prognostic indicators particularly important. The Ki67 and BCL2 proteins are known prognostic markers for different types of cancer. Ki67 is associated with cell proliferation, whereas BCL2 has antiproliferative roles. A combined marker based on these opposite functions might provide improved prognostic information in gastric cancer. Method: Ki67 and BCL2 expression was assessed in 276 gastric adenocarcinoma tissue microarrays. A Ki67/BCL2 index based on the relative expression of each protein was divided into low- and high-risk groups using receiver operating characteristic curves. Results: A high Ki67/BCL2 index significantly correlated with advanced stage, recurrence, intestinal type, high histologic grade, and lymphatic and perineural invasion (all p < 0.05). Univariate and multivariate analyses revealed a significant relationship between disease-free or overall survival and the Ki67/BCL2 index in intestinal-type gastric cancer (all p < 0.05). Conclusions: A combined marker using Ki67 and BCL2 could be a useful indicator for predicting survival in patients with intestinal-type gastric cancer.

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Section: Discussionmentioning

confidence: 99%

A High Ki67/BCL2 Index Could Predict Lower Disease-Free and Overall Survival in Intestinal-Type Gastric Cancer

Min

Kim²,

Son³

et al. 2017

Eur Surg Res

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“…Due to its aggregation in the target area with low radiation to the surrounding normal tissue and the profile of quick attenuation of radiation, 125 I radioactive particle is recognized as a potent tool in lung cancer treatment. CT guidance can obviously reduce operating time and radiation dose, and elevate puncture accuracy, resulting in small trauma, apparent curative effect, rapid recovery, and fewer adverse reactions [23,24]. This study retrospectively compared the effect of CT-guided 125 I radioactive particles and chemotherapy in the treatment of NSCLC.…”

Section: Discussionmentioning

confidence: 99%

The Mechanism of Computed Tomography-Guided 125I Particle in Treating Lung Cancer

Jin

Yang

et al. 2017

Med Sci Monit

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BackgroundThe incidence of malignant tumor has gradually increased. How to improve the survival and quality of life of patients who lose the opportunity for surgery or who are unwilling to receive surgery remains an obstacle. At present, 125I particle interstitial implant therapy has been applied in a variety of treatments of tumors. However, the mechanism of computed tomography (CT)-guided 125I particle therapy in lung cancer has not been fully elucidated.Material/MethodsA total of 42 patients with advanced non-small cell lung cancer were retrospectively analyzed between January 2013 and December 2013, including 19 patients who received CT-guided 125I particle therapy and 23 patients who received chemotherapy. Curative effect and adverse reactions at 6 months and 12 months were compared and analyzed. A rabbit lung cancer VX2 model was treated by 125I particle implantation therapy under CT guidance. The change in tumor volume was detected. Tumor cell apoptosis was tested by flow cytometry. Bcl-2 and Bax expression were determined by real-time polymerase chain reaction (PCR) and Western blot.Results125I particle therapy obviously reduced tumor volume after 6 months and 12 months. It showed significantly higher efficiency (57.9%, 57.9%) and control (78.9%, 73.7%) than the rates of efficiency and control in the chemotherapy group (P<0.05). 125I particle implantation therapy markedly suppressed rabbit VX2 transplanted tumor cell proliferation, promoted tumor regression, induced tumor cell apoptosis, reduced Bcl-2 expression, and upregulated Bax expression level (P<0.05).ConclusionsCT-guided 125I particle implantation therapy can inhibit tumor proliferation and growth by regulating the expression of apoptosis-related genes and proteins, which is a promising approach in lung cancer treatment.

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“…No. 352001) [59]. Simultaneously, it was also streaked on a BHI agar plate containing the same concentration of FBS and antibiotics.…”

Section: Culture Of Clinical Isolates In Liquid and Solid Growth Mediummentioning

confidence: 99%

“…The isolated H. pylori strains were analyzed for the growth pattern until 24 h. In brief, the isolates were cultured in a 14 ml round bottom snap cap tube (BD) in biological duplicates by setting initial OD 600 0.05, which correspond to approximately 80 million CFU per ml [59]. Further, they were incubated in the microaerophilic chamber, as mentioned above.…”

Section: Growth Curvementioning

confidence: 99%

“…A plate densitometry study on a BHI agar plate was performed to validate the growth pattern in the liquid medium. In order to check the growth of H. pylori (HB1, HB5, HJ9, HB14, and I10) on BHI agar plate, 1X10 7 bacteria were taken (OD 600 0.3 represents 500 million CFU per ml) [59] and suspended in 100 μL of BHI broth and then spread by using glass spreader. Further, images of plates were obtained at 0, 6, 12, and 24 h, and data were analyzed by measuring the mean grey value using Image J software (NIH) (Fig.…”

Section: Densitometry Of H Pylori Growth On Bhi Agar Platementioning

confidence: 99%

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Oral rinses in growth inhibition and treatment of Helicobacter pylori infection

et al. 2020

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Background: Helicobacter pylori (H. pylori) is well-known for its role in chronic gastritis and gastric cancer. Eradication of these carcinogenic bacteria from the gut is one of the challenges for clinicians. The complexity of treatment mainly owes to antibiotic resistance and relapse due to an additional reservoir in the oral cavity. Our study emphases the isolation of H. pylori from distinct habitats of the gut microenvironment (gastric biopsy and gastric juice) and its subsequent characterization. We have also evaluated the effect of various oral rinses on isolated H. pylori from different anatomical locations of included subjects. Results: The possible strains isolated from two different habitats of the same subject shows a striking difference in their growth pattern. Promisingly, some of the included oral rinses are efficient in growth inhibition as per recommended 30 s treatment. The subsequent evaluation shows that oral rinse B (among A-E) is most effective and down-regulates the expression of one of the potent H. pylori gene, CagA, in the infected gastric adenocarcinoma (AGS) cells. Conclusion:Our study, for the first time, revealed that H. pylori, isolated from the different habitat of the same subject, show a different growth pattern. The expression of H. pylori pathogenic gene (CagA) was down-regulated by the use of oral rinses. Hence, oral rinses will reduce the H. pylori in the oral cavity and help to control its migration from oral to the gastric compartment and may be used as an adjuvant treatment option for its reinfection.

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The Expression of Bcl-2 and BID in Gastric Cancer Cells

Cited by 38 publications

References 25 publications

A High Ki67/BCL2 Index Could Predict Lower Disease-Free and Overall Survival in Intestinal-Type Gastric Cancer

A High Ki67/BCL2 Index Could Predict Lower Disease-Free and Overall Survival in Intestinal-Type Gastric Cancer

The Mechanism of Computed Tomography-Guided 125I Particle in Treating Lung Cancer

Oral rinses in growth inhibition and treatment of Helicobacter pylori infection

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