The expression of aquaporin 8 and aquaporin 9 in fetal membranes and placenta in term pregnancies complicated by idiopathic polyhydramnios

Zhu, Xiongzhao; Jiang, Shanshan; Hu, Yingchun; Zheng, Xiaoqun; Zou, Shuangwei; Wang, Yuhuan; Zhu, Xueqiong

doi:10.1016/j.earlhumdev.2010.07.012

Cited by 53 publications

(46 citation statements)

References 24 publications

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“…; Zhu et al. ), whereas patients with oligohydramnios (AFVs below normal) have reduced AQP1, AQP3, AQP8, and AQP9 mRNA expression (Zhu et al. ; Jiang et al.…”

Section: Introductionmentioning

confidence: 99%

Differential expression and regional distribution of aquaporins in amnion of normal and gestational diabetic pregnancies

Beardall

Brace

et al. 2015

Physiol Rep

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The region of the amnion overlying the placenta plays an active role in fluid exchange between amniotic fluid and fetal blood perfusing the surface of the placenta, whereas little transfer occurs across the reflected amnion that contacts the membranous chorion. Because aquaporins (AQPs) facilitate rapid movement of water across cells, we hypothesized that AQP gene expression in placental amnion is higher than in reflected amnion. Furthermore, because gestational diabetes mellitus (GDM) is often associated with polyhydramnios, we hypothesized that amnion AQP gene expression is reduced when amniotic fluid volume is elevated. Human placental and reflected amnion were obtained at cesarean delivery and subjected to relative quantitation of AQP mRNA by real-time RT-qPCR and proteins by western immunoblot. Amnion mRNA levels of five AQPs differed by up to 400-fold (P < 0.001), with AQP1 and AQP3 most abundant, AQP8 least and AQP9 and AQP11 intermediately expressed. Aquaporin proteins showed a similar profile. Aquaporin mRNA abundance was higher (P < 0.001) in placental than reflected amnion, whereas protein levels were lower (P < 0.01). In GDM pregnancies, neither AQP mRNA nor protein levels were different from normal. There was no correlation between AQP mRNA or protein levels with the amniotic fluid index in normal or GDM subjects. We conclude that there is a strong differential expression profile among individual AQPs and between regions of the amnion. These findings suggest differences in contribution of individual AQPs to water transport in the two regions of the amnion. Furthermore, AQP expression in the amnion is not altered in patients with GDM.

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“…; Zhu et al. ), whereas patients with oligohydramnios (AFVs below normal) have reduced AQP1, AQP3, AQP8, and AQP9 mRNA expression (Zhu et al. ; Jiang et al.…”

Section: Introductionmentioning

confidence: 99%

Differential expression and regional distribution of aquaporins in amnion of normal and gestational diabetic pregnancies

Beardall

Brace

et al. 2015

Physiol Rep

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“…The AQPs are known to play a major role in the regulation of water homeostasis in the body and to participate in the regulation of the amniotic volume (19). An expression of AQPs 1, 3, 8, and 9 in chorio-amniotic membranes has already been reported in healthy pregnancy (10); an increase in their expression has been observed in idiopathic polyhydramnios (12)(13)(14). In addition, polyhydramnios is known to frequently be associated with diabetes (5,6).…”

Section: Discussionmentioning

confidence: 99%

“…They have reported an increase in the expression of the AQPs usually found on these membranes at full term. The expression of AQPs 1 (12), 3, and 9 (13) increases in both amnion and chorion; that of AQP8 increases only in the amnion (14). For Mann and coworkers, the increased expression of AQP1 is seen as a mechanism designed to compensate for the increase in volume of the amniotic fluid, rather than causing it (12).…”

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confidence: 99%

Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin

Bouvier

Rouzaire

Marceau

et al. 2015

The Journal of Clinical Endocrinology &Amp; Metabolism

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Context: During pregnancy, aquaporins (AQPs) expressed in fetal membranes are essential for controlling the homeostasis of the amniotic volume, but their regulation by insulin was never explored in diabetic women. Objective:The aim of our study was to investigate the involvement of AQPs 1, 3, 8, and 9 expressed in fetal membranes in diabetic parturient women and the control of their expression by insulin. Design and Participants:From 129 fetal membranes in four populations (controls, type 1, type 2 [T2D], and gestational diabetes [GD]), we established an expression AQP profile. In a second step, the amnion was used to study the control of the expression and functions of AQPs 3 and 9 by insulin. Main Outcomes and Measures:The expression of transcripts and proteins of AQPs was studied by quantitative RT-PCR and ELISA. We analyzed the regulation by insulin of the expression of AQPs 3 and 9 in the amnion. A tritiated glycerol test enabled us to measure the impact of insulin on the functional characteristics. Using an inhibitor of phosphatidylinositol 3-kinase, we analyzed the insulin intracellular signaling pathway. Results:The expression of AQP3 protein was significantly weaker in groups T2D and GD. In nondiabetic fetal membranes, we showed for the amnion (but not for the chorion) a significant repression by insulin of the transcriptional expression of AQPs 3 and 9, which was blocked by a phosphatidylinositol 3-kinase inhibitor. Conclusion:In fetal membranes, the repression of AQP3 protein expression and functions observed in vivo is allowed by the hyperinsulinism described in pregnant women with T2D or GD. (J Clin Endocrinol Metab 100: E1270 -E1279, 2015)

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“…However, Mann et al 42 subsequently demonstrated in further studies that, in fact, aquaporin 1 expression is increased in pregnancies complicated by idiopathic polyhydramnios and is likely a compensatory response to the increased fluid volume in humans, rather than a cause. Zhu et al 40 found an increase in the expression of aquaporin 8 in the amnion and aquaporin 9 within the amnion and chorion in the setting of idiopathic hydramnios, which may indicate an adaptive change in the fetal membranes and placenta to potentially regulate the AFV. The exact mechanism by which idiopathic hydramnios develops and is regulated appears to be complex and remains incompletely understood.…”

Section: Etiology Of Polyhydramniosmentioning

confidence: 99%

Clinical Relevance of Sonographically Estimated Amniotic Fluid Volume

Sandlin

Chauhan

Magann

2013

Journal of Ultrasound in Medicine

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Polyhydramnios is an excessive amount of amniotic fluid within the amniotic cavity. The etiology of polyhydramnios may be idiopathic, the consequence of fetal structural anomalies, or the consequence of various fetal and maternal conditions. The clinical importance of polyhydramnios is found in its association with adverse pregnancy outcomes and the risk of perinatal mortality. The antenatal management of polyhydramnios can be challenging as there are no formalized guidelines on the topic. The purpose of this review is to provide a literature‐based overview on the subject of polyhydramnios in singleton pregnancies, demonstrate its clinical implications, and describe a practical approach to its management.

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The expression of aquaporin 8 and aquaporin 9 in fetal membranes and placenta in term pregnancies complicated by idiopathic polyhydramnios

Cited by 53 publications

References 24 publications

Differential expression and regional distribution of aquaporins in amnion of normal and gestational diabetic pregnancies

Differential expression and regional distribution of aquaporins in amnion of normal and gestational diabetic pregnancies

Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin

Clinical Relevance of Sonographically Estimated Amniotic Fluid Volume

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