The expression of alfalfa MsPP2CA1 gene confers ABA sensitivity and abiotic stress tolerance on Arabidopsis thaliana

Wang, Dong; Liu, Xijiang; Lv, Jiao; Gao, Tianxue; Song, Yuhan

doi:10.1016/j.plaphy.2019.09.004

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…Plant inoculation with halotolerant bacteria is one the most sustainable way to cope with the numerous deleterious effects of salinity [50][51][52][53]. Several microorganisms have been reported as promoters of plant growth under saline stress [12,16,54].…”

Section: Discussionmentioning

confidence: 99%

“…In light of the PGP traits mentioned above, the improvement of plant growth under halo stress could be the result of an improvement of nutrients uptake, hormones regulation [90,91], and enzymatic activities [92]. Salinity negatively affects plant growth and yield [93,94] with a high negative impact on the root system [51]. The presence of NaCl affects also aerial parts, with a reduction in photosynthesis due to a decrease of chlorophylls content [7,95,96].…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

et al. 2021

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This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

et al. 2021

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“…The cycling regime comprised an initial denaturation (95°C/2 min), followed by 45 cycles of 95°C/10 s, 60°C/20 s, and 72°C/20 s. Melting curve analysis was performed over the range of 80-95°C at 0.5°C intervals. The MsACTIN gene was used as a reference (Dong et al 2019), and relative transcript abundances were derived using the 2 − δδCt method (Livak et al 2001). Three technical replicates were performed for each of the three biological replicates.…”

Section: Transcriptional Analysis By Quantitative Real Time Pcr (Qrt-...mentioning

confidence: 99%

“…The levels of malondialdehyde (MDA), H 2 O 2 and chlorophyll, along with the activity of the ROS scavenging enzymes peroxide (POD), were assessed using commercially available kits (Jiancheng, Nanjing, China). The malondialdehyde (MDA) and H 2 O 2 levels of epidermal peels required for the analysis of stomatal aperture were determined using a published method (Dong et al 2019).…”

Section: The Quanti Cation Of Reactive Oxygen Species (Ros) Malondial...mentioning

confidence: 99%

Constitutive expression of MsPROPEP1 enhances salinity and drought tolerance in alfalfa (Medicago sativa)

Tang,

Guo,

Shang

et al. 2024

Preprint

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Plant peptide hormones have various important roles in plant development, defense against pathogens, and tolerance to abiotic stress. However, only a limited number of hormone-like peptides have been proven to contribute to salt and drought stress tolerance in plants other than Arabidopsis. In this study, we present the isolation and characterization of MsPROPEP1, a propeptide precursor gene obtained from the legume pasture Medicago sativa. The transcription of the MsPROPEP1 was found to be inducible by NaCl, polyethylene glycol (PEG), and abscisic acid (ABA). The constitutive expression of MsPROPEP1 in alfalfa seedlings mitigated the restriction on plant growth imposed by either salinity or osmotic stress and raised their sensitivity to ABA in promoting stomatal closure. In addition, we synthesized MsPep1 peptide and found that the application of MsPep1 enhanced tolerance to stress induced by NaCl and PEG. In transgenic plants, many ABA-dependent stress-responsive genes are activated; this is known to promote the expression of peroxidase which plays a role in reactive oxygen scavenging. Our findings suggest that MsPROPEP1 is a candidate for the genetic manipulation of salinity and drought tolerance in legume species.

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“…Many studies have confirmed that the overexpression of transcription factor (TF) genes (i.e., MxbHLH18 and MxWRKY53) [23,24], antioxidant enzyme genes (i.e., AhCuZnSOD and AtAPX3) [25,26], osmotic regulation genes (i.e., MdHXK1 and AtBAG6) [27,28] and ion transporter genes (i.e., OsNHX7 and CNGC1) [29,30] changed salinity tolerance in transgenic plants. In alfalfa, several genes that participate in the salinity stress response, such as MsCBL4 [31], MsPP2CA1 [32] and MsPOD [33] have also been reported.…”

mentioning

confidence: 99%

Comparative physiology and transcriptome analysis to identify the important coding and non-coding RNAs imparting tolerance to salinity stress in alfalfa (Medicago sativa L.)

Rong

et al. 2023

Preprint

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Background: Salinity is among the most harmful agents that impacts alfalfa’s productivity. However, little is known regarding how salinity tolerance trait is regulated by lncRNAs in alfalfa. To evaluate the mRNAs and lncRNAs associated with tolerance to salinity stress in alfalfa, the root transcriptomics of two alfalfa cultivars differing in salinity tolerance, i.e., GN5 (tolerant to salinity) and GN3 (sensitive to salinity), were investigated under 0 and 150 mM NaCl treatments. Results: A total of 172,986 mRNAs and 117,677 lncRNAs were identified, of which 2288 mRNAs and 1466 lncRNAs were significantly differentially expressed in four comparisons (GN5-150 vs. GN5-0, GN3-150 vs. GN3-0, GN5-0 vs. GN3-0, and GN5-150 vs. GN3-150). Based on GO and KEGG pathway analysis, the expression levels of several osmotic and ionic stress-related genes, including HPCA1-LRR, PP2C60, PP2C71, CRK1, APX3, HXK2, BAG6, and ARF1, were higher in GN5 than in GN3. CNGC1 was significantly downregulated by NaCl treatment in GN5. The co-expressed network analysis indicated that TCONS_00113549, TCONS_00399794, TCONS_00297228, TCONS_00004647, TCONS_00033214 and TCONS_00285177 in alfalfa roots regulated 66 genes including the four genes mentioned above. Therefore, the above nine genes and six lncRNAs might contribute to the difference in salinity tolerance between GN5 and GN3. Conclusions: We discovered a broad survey of mRNAs and lncRNAs associated with the difference of salinity tolerance between GN5 and GN3. These findings would be useful for further functional analysis as potential targets to improve resistance to salinity stress via genetic engineering.

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The expression of alfalfa MsPP2CA1 gene confers ABA sensitivity and abiotic stress tolerance on Arabidopsis thaliana

Cited by 10 publications

References 37 publications

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Constitutive expression of MsPROPEP1 enhances salinity and drought tolerance in alfalfa (Medicago sativa)

Comparative physiology and transcriptome analysis to identify the important coding and non-coding RNAs imparting tolerance to salinity stress in alfalfa (Medicago sativa L.)

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