The expression of ALDH1 in cervical carcinoma

Yao, Tingting; Chen, Qing; Zhang, Bingzhong; Zhou, Hui; Lin, Zhongqiu

doi:10.12659/msm.881886

Cited by 33 publications

(21 citation statements)

References 16 publications

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“…We plan to address this question in our future studies. Although a previous study demonstrated ALDH1 expression in cervical carcinoma cells (Yao et al, 2011), suggesting the presence of cancer stem cells, we are the first to show that ALDH1-positive cervical cancer cells were significantly more tumorigenic and invasive compared to ALDH1-negative cells. Our future studies aim to dissect the mechanisms underlying ALDH1-mediated increase in proliferation, invasion and tumorigenesis in vitro and in cervical carcinoma tissue from metastatic and non-metastatic cervical carcinoma patients.…”

Section: Discussioncontrasting

confidence: 56%

Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells

Rao¹,

Yao²,

Zhang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Tumor formation and growth is dictated by a very small number of tumor cells, called cancer stem cells, which are capable of self-renewal. The genesis of cancer stem cells and their resistance to conventional chemotherapy and radiotherapy via mechanisms such as multidrug resistance, quiescence, enhanced DNA repair abilities and anti-apoptotic mechanisms, make it imperative to develop methods to identify and use these cells as diagnostic or therapeutic targets. Aldehyde dehydrogenase 1 (ALDH1) is used as a cancer stem cell marker. In this study, we evaluated ALDH1 expression in CaSki, HeLa and SiHa cervical cancer cells using the Aldefluor method to isolate ALDH1-positive cells. We showed that higher ALDH1 expression correlated with significantly higher rates of cell proliferation, microsphere formation and migration. We also could demonstrate that SiHa-ALDH1-positive cells were significantly more tumorigenic compared to SiHa-ALDH1-negative cells. Similarly, SiHa cells overexpressing ALDH1 were significantly more tumorigenic and showed higher rates of cell proliferation and migration compared to SiHa cells where ALDH1 expression was knocked down using a lentivirus vector. Our data suggested that ALDH1 is a marker of cervical cancer stem cells and expand our understanding of its functional role.

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Section: Discussioncontrasting

confidence: 56%

Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells

Rao¹,

Yao²,

Zhang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…They found lower levels of CD133 in HeLa, SiHa and CaSki cervical cell lines similar to showed by López et al . (2012) [44], define ALDH1 as a marker for identifying CSC and suggest that cervical carcinoma contains a small subpopulation of cells with a cancer stem cell-like phenotype [45]. …”

Section: Markers Of Cervical Cancer Stem Cellmentioning

confidence: 99%

Human Papillomavirus Infections and Cancer Stem Cells of Tumors from the Uterine Cervix

López

Ruíz²,

Organista‐Nava³

et al. 2012

TOVJ

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Different rate of development of productive infections (as low grade cervical intraepithelial neoplasias), or high grade lesions and cervical malignant tumors associated with infections of the Transformation zone (TZ) by High-Risk Human Papillomavirus (HR-HPV), could suggest that different epithelial host target cells could exist. If there is more than one target cell, their differential infection by HR-HPV may play a central role in the development of cervical cancer. Recently, the concept that cancer might arise from a rare population of cells with stem cell-like properties has received support in several solid tumors, including cervical cancer (CC). According to the cancer stem cell (CSC) hypothesis, CC can now be considered a disease in which stem cells of the TZ are converted to cervical cancer stem cells by the interplay between HR-HPV viral oncogenes and cellular alterations that are thought to be finally responsible for tumor initiation and maintenance. Current studies of CSC could provide novel insights regarding tumor initiation and progression, their relation with viral proteins and interplay with the tumor micro-environment. This review will focus on the biology of cervical cancer stem cells, which might contribute to our understanding of the mechanisms responsible for cervical tumor development.

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“…Recently, immunohistochemical staining for ALDH has been used in detecting CSCs in various types of cancer, such as cervical, endometrial, pancreatic, breast, and hepatocellular adenocarcinoma. [21][22][23][24] Like any other technique, the ALDH assay has some limitations. Aldehyde dehydrogenase activity is affected by chemotherapy.…”

Section: In Vitro Methodologies To Demonstrate Cancer Stem Cells: Funmentioning

confidence: 99%

Cancer Stem Cells: A Review of Potential Clinical Applications

Podberezin

Wen

Chung-Che

2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.-Cancer stem cells (CSCs) comprise a minor cell population in a tumor; however, they possess selfrenewal capacity and are responsible for tumor recurrence and the emerging issue of tumor resistance. Despite recent advances in the study of pathogenesis and mechanisms of CSC-mediated disease recurrence and multidrug resistance, many questions remain unanswered.Objectives.-To provide an overview of CSC theory and to describe major methods of CSC detection and isolation, with the emphasis on those techniques that are potentially relevant in clinical laboratory practice. Particular attention is given to CSC markers, such as cancer testis antigens, which could become promising targets in the development of immunotherapy in settings of minimal residual disease.Data Sources.-The review is based on analysis of peerreviewed literature cited in PubMed, as well as preliminary results of studies conducted in our laboratory.Conclusions.-Despite a lack of consensus in the scientific community on research methodology, CSCs have demonstrated significant potential as therapeutic targets in the treatment of cancer. Further research of CSC biology and markers will eventually lead to the development of novel therapeutic approaches for targeting these cells to treat resistant and recurrent tumors and minimal residual disease.

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The expression of ALDH1 in cervical carcinoma

Cited by 33 publications

References 16 publications

Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells

Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells

Human Papillomavirus Infections and Cancer Stem Cells of Tumors from the Uterine Cervix

Cancer Stem Cells: A Review of Potential Clinical Applications

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