The Expression of Aldehyde Dehydrogenase Family in Breast Cancer

Qiu, Yan; Pu, Tianjie; Li, Li; Cheng, Fei; Lu, Changli; Sun, Long; Teng, Xiao; Feng, Yan; Bu, Hong

doi:10.4048/jbc.2014.17.1.54

Cited by 22 publications

(22 citation statements)

References 24 publications

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“…ALDH activity measured by the ALDEFLUOR assay is used as a CSC marker in many cancer types [24]. In particular, ALDH1A3 is a predictive marker of a poor clinical outcome in breast cancers [36]. …”

Section: Introductionmentioning

confidence: 99%

Induced Expression of Cancer Stem Cell Markers ALDH1A3 and Sox-2 in Hierarchical Reconstitution of Apoptosis-resistant Human Breast Cancer Cells

Kashii-Magaribuchi

Takeuchi

Haisa

et al. 2016

Acta Histochemica et Cytochemica

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We established an experimental system that can induce p53-dependent apoptosis by doxycycline treatment to analyze characteristics of the apoptosis-resistant cancer cell subpopulation in the human breast cancer cell line HCC1937. Expression patterns of the stem cell markers, ALDH1A3 and Sox-2, the luminal differentiation marker, GATA3 and the proliferation index marker, Ki-67 were analyzed using immunostaining and fluorescence-activated cell sorting (FACS). After doxycycline treatment, the number of viable cells was gradually decreased over seven days in a time-dependent manner due to p53-induced apoptosis; however, the number of smaller-sized ALDH1A3+ cells assessed by immunostaining increased sharply after 1 day of doxycycline treatment, suggesting their apoptosis-resistant nature. The expression of ALDH1A3 was also detected in 78% of small-sized Ki-67+ proliferating progenitor cells, followed by the transient expression of GATA3, which presumably indicated the ability to differentiate into luminal progenitor cells. Although 42.2–58.5% of residual cells were positive for both ALDH1A3 and GATA3, their expression patterns exhibited an inverse correlation. The expression pattern of another stem cell marker, Sox-2, was similar, but more drastically altered after p53 induction compared with ALDH1A3. These findings may aid in understanding the hierarchical responses of cancer stem cells to therapeutic stresses.

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Section: Introductionmentioning

confidence: 99%

Induced Expression of Cancer Stem Cell Markers ALDH1A3 and Sox-2 in Hierarchical Reconstitution of Apoptosis-resistant Human Breast Cancer Cells

Kashii-Magaribuchi

Takeuchi

Haisa

et al. 2016

Acta Histochemica et Cytochemica

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“…The ALDH superfamily currently consists of 19 known putatively functional genes in 11 families and 4 subfamilies with distinct chromosomal locations [23][24][25]. Several studies have explored the biological significance of ALDH in cancers such as head and neck cancer, colon cancer, breast cancer, papillary thyroid carcinoma, and specifically lung cancer, where they have provided supportive evidence for the association between ALDH activity and lung cancer stem cells [26][27][28][29][30][31][32]. ALDH1A1 seems to be co-expressed with other NSCLC stem cell markers such as leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) in NSCLC tissues, and their expression is significantly associated with stage disease and poor prognosis [33].…”

Section: Aldh Family Proteinsmentioning

confidence: 99%

Molecular Biology of Lung Cancer and Future Perspectives for Screening

Tarro¹,

Paolini²,

Rossi³

2019

Mass Spectrometry - Future Perceptions and Applications

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Lung cancer patients have the highest mortality among patients with solid tumors worldwide and their prognosis is strictly stage-associated. However, only 15-20% of patients are diagnosed in stage I, since these early tumors are frequently asymptomatic. Early detection of lung cancer, which allows effective therapeutic intervention, is a promising approach to lowering its mortality rate. However, conventional diagnostic methods for lung cancer, such as chest X-ray and CT of the chest, produce high costs and potentially false-positive results. Thus, the discovery of highly sensitive, specific, noninvasive, and cost-effective lung cancer biomarkers combined with conventional approaches, such as X-rays, may improve the sensitivity of lung cancer screening. Herein, we summarize the most recent studies about the molecular pathology of lung cancer and discuss the advancements expected in the near future, including the potential biomarkers and liquid biopsy approaches for the detection of lung cancer in populations at risk of developing this disease.

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“…Significantly higher activity of alcohol dehydrogenase enzymes has been shown in breast, ovarian, colorectal, and cervical cancer tissues compared to healthy human tissues (Jelski, Chrostek, Szmitkowski, & Markiewicz, 2006; Jelski, Zalewski, Chrostek, & Szmitkowski, 2004; Orywal, Jelski, Zdrodowski, & Szmitkowski, 2011, 2013). ALDH1A3 expression was also high in breast cancer tissues and ALDH1A3 levels significantly correlated with distant metastasis (Qiu, Pu, Li, Cheng, Lu, Sun, et al, 2014). Further, ALDH1A1 expression was observed in 63.0% (92 of 146) of human breast invasive ductal carcinoma tissues (Zhong, Lin, Shen, Zhou, Mao, Guan, et al, 2013).…”

Section: Cyp26 and Retinoic Acid In Cancermentioning

confidence: 99%

Role of Retinoic Acid-Metabolizing Cytochrome P450s, CYP26, in Inflammation and Cancer

Stevison

Jing

Tripathy

et al. 2015

Advances in Pharmacology

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Vitamin A (retinol) and its active metabolite, all-trans-retinoic acid (atRA), play critical roles in regulating the differentiation, growth and migration of immune cells. Similarly, as critical signaling molecules in the regulation of the cell cycle, retinoids are important in cancers. Concentrations of atRA are tightly regulated in tissues, predominantly by the availability of retinol, synthesis of atRA by ALDH1A enzymes and metabolism and clearance of atRA by CYP26 enzymes. The ALDH1A and CYP26 enzymes are expressed in several cell types in the immune system and in cancer cells. In the immune system the ALDH1A and CYP26 enzymes appear to modulate RA concentrations. Consequently, alterations in the activity of ALDH1A and CYP26 enzymes are expected to change disease outcomes in inflammation. There is increasing evidence from various disease models of intestinal and skin inflammation that treatment with atRA has a positive effect on disease markers. However, whether aberrant atRA concentrations or atRA synthesis and metabolism play a role in inflammatory disease development and progression is not well understood. In cancers, especially in acute promyelocytic leukemia and neuroblastoma, increasing intracellular concentrations of atRA appears to provide clinical benefit. Inhibition of the CYP26 enzymes to increase atRA concentrations and combat therapy resistance has been pursued as a drug target in these cancers. This chapter covers the current knowledge of how atRA and retinol regulate the immune system and inflammation, how retinol and atRA metabolism is altered in inflammation and cancer and what roles atRA metabolizing enzymes have in immune responses and cancers.

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The Expression of Aldehyde Dehydrogenase Family in Breast Cancer

Cited by 22 publications

References 24 publications

Induced Expression of Cancer Stem Cell Markers ALDH1A3 and Sox-2 in Hierarchical Reconstitution of Apoptosis-resistant Human Breast Cancer Cells

Induced Expression of Cancer Stem Cell Markers ALDH1A3 and Sox-2 in Hierarchical Reconstitution of Apoptosis-resistant Human Breast Cancer Cells

Molecular Biology of Lung Cancer and Future Perspectives for Screening

Role of Retinoic Acid-Metabolizing Cytochrome P450s, CYP26, in Inflammation and Cancer

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