The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh

Lång, V.; Palva, E. Tapio

doi:10.1007/bf00027165

Cited by 392 publications

(264 citation statements)

References 59 publications

(104 reference statements)

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“…Other genes such as ACX2 and ACX4 required water losses of at least 40%, a result similar to that observed for rab18, which is a typical marker of dehydration and cold acclimation responses in Arabidopsis (Lang and Palva, 1992). Genes such as ACX3 and KAT5 were the less sensitive to dehydration, requiring more than 80% of water loss to be induced.…”

Section: Resultssupporting

confidence: 77%

Gene-Specific Involvement of β-Oxidation in Wound-Activated Responses in Arabidopsis

et al. 2004

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The coordinated induced expression of b-oxidation genes is essential to provide the energy supply for germination and postgerminative development. However, very little is known about other functions of b-oxidation in nonreserve organs. We have identified a gene-specific pattern of induced b-oxidation gene expression in wounded leaves of Arabidopsis. Mechanical damage triggered the local and systemic induction of only ACX1 among acyl-coenzyme A oxidase (ACX) genes, and KAT2/ PED1 among 3-ketoacyl-coenzyme A thiolase (KAT) genes in Arabidopsis. In turn, wounding induced KAT5/PKT2 only systemically. Although most of the b-oxidation genes were activated by wound-related factors such as dehydration and abscisic acid, jasmonic acid (JA) induced only ACX1 and KAT5. Reduced expression of ACX1 or KAT2 genes, in transgenic plants expressing their corresponding mRNAs in antisense orientation, correlated with defective wound-activated synthesis of JA and with reduced expression of JA-responsive genes. Induced expression of JA-responsive genes by exogenous application of JA was unaffected in those transgenic plants, suggesting that ACX1 and KAT2 play a major role in driving wound-activated responses by participating in the biosynthesis of JA in wounded Arabidopsis plants.Plants often undergo the onslaught of chewing insects or larger herbivores that cause damage to the leaves. Preexisting physical barriers may not be enough to prevent injuries and thus, plants require active inducible defense mechanisms. Moreover, once an injury occurs there is no possibility of wound healing by mobilization of specialized cells as it occurs in animals. In plants, every cell has become competent for the activation of wound-triggered defense responses. Wound-activated defense relies on the production or release of signals in the damaged tissues and the local and systemic activation of signaling pathways (Leó n et al., 2001). Wound-activated signaling pathways usually lead to the transcriptional activation of defense-related genes. In addition, damaged areas undergo a severe disorder of tissue and cellular structures that is accompanied by a drastic loss of water (Reymond et al., 2000). Wound-activated gene expression seems to be the result of the combined action of damage and water stress of the wounded leaf (Reymond et al., 2000), processes that require the synthesis, accumulation, and perception of jasmonic acid (JA) and abscisic acid (ABA; Peñ a- Cortés et al., 1995; Bergey et al., 1996). The signaling function of jasmonates in wound-activated defense has been extensively documented (Turner et al., 2002). Besides, jasmonates are also involved in pathogen-triggered defense in coordination with the function of salicylic acid (SA; Glazebrook et al., 2003).Plants synthesize jasmonates from linolenic acid through the octadecanoid pathway (Schaller, 2001). This is a complex metabolic pathway involving the participation of different subcellular organelles. The release of linolenic acid from membrane lipids and subsequent redox reactions to 12-oxo...

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Section: Resultssupporting

confidence: 77%

Gene-Specific Involvement of β-Oxidation in Wound-Activated Responses in Arabidopsis

et al. 2004

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“…To this end, we used qRT-PCR to analyze the expression of the ABA-and drought-responsive RAB18, P5CS1, RD29B, KIN1, RD29A, and RD22 genes, in wild type, single, and double mutants. These gene markers have been widely used to monitor the ABA and stress response pathways in plants (Kurkela and Franck, 1990;Lang and Palva, 1992;Yamaguchi-Shinozaki and Shinozaki, 1994;Strizhov et al, 1997;Abe et al, 2003). In general, in the absence of ABA or stress treatments, these gene markers show a low expression, which is strongly upregulated in response to the inductive signal.…”

Section: Enhanced Expression Of Aba-inducible Genes In Pp2c Mutants Amentioning

confidence: 99%

Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1

et al. 2006

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Abscisic acid (ABA) plays a key role in plant responses to abiotic stress, particularly drought stress. A wide number of ABAhypersensitive mutants is known, however, only a few of them resist/avoid drought stress. In this work we have generated ABA-hypersensitive drought-avoidant mutants by simultaneous inactivation of two negative regulators of ABA signaling, i.e. the protein phosphatases type 2C (PP2Cs) ABA-INSENSITIVE1 (ABI1) and HYPERSENSITIVE TO ABA1 (HAB1). Two new recessive loss-of-function alleles of ABI1, abi1-2 and abi1-3, were identified in an Arabidopsis (Arabidopsis thaliana) T-DNA collection. These mutants showed enhanced responses to ABA both in seed and vegetative tissues, but only a limited effect on plant drought avoidance. In contrast, generation of double hab1-1 abi1-2 and hab1-1 abi1-3 mutants strongly increased plant responsiveness to ABA. Thus, both hab1-1 abi1-2 and hab1-1 abi1-3 were particularly sensitive to ABA-mediated inhibition of seed germination. Additionally, vegetative responses to ABA were reinforced in the double mutants, which showed a strong hypersensitivity to ABA in growth assays, stomatal closure, and induction of ABA-responsive genes. Transpirational water loss under drought conditions was noticeably reduced in the double mutants as compared to single parental mutants, which resulted in reduced water consumption of whole plants. Taken together, these results reveal cooperative negative regulation of ABA signaling by ABI1 and HAB1 and suggest that fine tuning of ABA signaling can be attained through combined action of PP2Cs. Finally, these results suggest that combined inactivation of specific PP2Cs involved in ABA signaling could provide an approach for improving crop performance under drought stress conditions.

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“…7). RAB18 (Lang and Palva, 1992;Parcy et al, 1994) is an ABA-inducible gene whose expression is drastically inhibited both in abi1-1 and abi2-1 mutants (Leung et al, 1997), and accumulation of P5CS1 mRNA is induced by drought, salinity, and ABA and is reduced by approximately 50% in the abi1-1 mutant compared with wild-type plants (Strizhov et al, 1997). Similarly, RD29A and RD29B are cold-, drought-, and ABA-inducible genes that contain ABA-responsive elements in their promoters.…”

Section: Altered Expression Of Aba-responsive Genes In Pyl8/rcar3 Tramentioning

confidence: 99%

The Nuclear Interactor PYL8/RCAR3 ofFagus sylvaticaFsPP2C1 Is a Positive Regulator of Abscisic Acid Signaling in Seeds and Stress

et al. 2009

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The functional protein phosphatase type 2C from beechnut (Fagus sylvatica; FsPP2C1) was a negative regulator of abscisic acid (ABA) signaling in seeds. In this report, to get deeper insight on FsPP2C1 function, we aim to identify PP2C-interacting partners. Two closely related members (PYL8/RCAR3 and PYL7/RCAR2) of the Arabidopsis (Arabidopsis thaliana) BetV I family were shown to bind FsPP2C1 in a yeast two-hybrid screening and in an ABA-independent manner. By transient expression of FsPP2C1 and PYL8/RCAR3 in epidermal onion (Allium cepa) cells and agroinfiltration in tobacco (Nicotiana benthamiana) as green fluorescent protein fusion proteins, we obtained evidence supporting the subcellular localization of both proteins mainly in the nucleus and in both the cytosol and the nucleus, respectively. The in planta interaction of both proteins in tobacco cells by bimolecular fluorescence complementation assays resulted in a specific nuclear colocalization of this interaction. Constitutive overexpression of PYL8/RCAR3 confers ABA hypersensitivity in Arabidopsis seeds and, consequently, an enhanced degree of seed dormancy. Additionally, transgenic 35S:PYL8/RCAR3 plants are unable to germinate under low concentrations of mannitol, NaCl, or paclobutrazol, which are not inhibiting conditions to the wild type. In vegetative tissues, Arabidopsis PYL8/RCAR3 transgenic plants show ABA-resistant drought response and a strong inhibition of early root growth. These phenotypes are strengthened at the molecular level with the enhanced induction of several ABA response genes. Both seed and vegetative phenotypes of Arabidopsis 35S:PYL8/RCAR3 plants are opposite those of 35S: FsPP2C1 plants. Finally, double transgenic plants confirm the role of PYL8/RCAR3 by antagonizing FsPP2C1 function and demonstrating that PYL8/RCAR3 positively regulates ABA signaling during germination and abiotic stress responses.

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The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh

Cited by 392 publications

References 59 publications

Gene-Specific Involvement of β-Oxidation in Wound-Activated Responses in Arabidopsis

Gene-Specific Involvement of β-Oxidation in Wound-Activated Responses in Arabidopsis

Enhancement of Abscisic Acid Sensitivity and Reduction of Water Consumption in Arabidopsis by Combined Inactivation of the Protein Phosphatases Type 2C ABI1 and HAB1

The Nuclear Interactor PYL8/RCAR3 ofFagus sylvaticaFsPP2C1 Is a Positive Regulator of Abscisic Acid Signaling in Seeds and Stress

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