The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection

Matsye, Prachi D.; Lawrence, Gary W.; Youssef, Reham M.; Kim, Kyung-Hwan; Lawrence, Katheryn S.; Matthews, Benjamin F.; Klink, Vincent P.

doi:10.1007/s11103-012-9932-z

Cited by 68 publications

(196 citation statements)

References 149 publications

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“…The pRAP15 vector was originally developed for Agrobacterium rhizogenes-mediated root genetic engineering experiments in G. max (Matsye et al 2012;Matthews et al 2013Matthews et al , 2014Pant, Matsye, et al 2014;. The sequence of the pRAP15 vector used in the study was determined prior to the analysis (Eurofins MWG Operon, Huntsville, Alabama; Matsye et al 2012).…”

Section: Prap15 Plasmidmentioning

confidence: 99%

“…The sequence of the pRAP15 vector used in the study was determined prior to the analysis (Eurofins MWG Operon, Huntsville, Alabama; Matsye et al 2012). From that sequence information, the vector map for pRAP15 was made by taking its confirmed sequence information and entering it into PlasMapper Version 2.0 (Dong et al 2004).…”

Section: Prap15 Plasmidmentioning

confidence: 99%

“…The expression of the cassette is driven by the figwort mosaic virus sub-genomic transcript (FMV-sgt) promoter (Bhattacharyya et al 2002). By using FMV-sgt as the promoter, the cassette is designed to drive the overexpression of full length genes and maintain high expression throughout plant-organism interactions (Klink et al 2008(Klink et al , 2009Matsye et al 2012;Pant, Matsye, et al 2014;). The pRAP15 vector was engineered to have the tetracycline resistance gene for bacterial selection, found to be important for work with A. rhizogenes (Klink et al 2008).…”

Section: Prap15 Plasmidmentioning

confidence: 99%

“…The development of the Gateway® system made that goal a reality (Curtis & Grossniklaus 2003). Later experiments adapted the Gateway® system, so that it could be used in agriculturally relevant plants (Klink et al 2009;Matsye et al 2012). Eventually, high throughput root genomics was demonstrated which ushered in a new era of functional root studies (Matthews et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…A comparative analysis, including the efficiency of transformation in G. hirsutum, is presented showing the rate by which roots useful for experimentation are generated. The system presented here differs from other hairy root-based systems developed in G. hirsutum in that, by using eGFP as a reporter system, the experiments can be performed under nonaxenic conditions throughout the study (Triplett et al 2008;Kim et al 2011;Matsye et al 2012;Pant, Matsye, et al 2014;. Furthermore, the platform is Gateway® compatible which simplifies the isolation and study of hundreds to thousands of genes identified in bioinformatics analyses (Matsye et al 2012;Matthews et al 2013;Pant, Matsye, et al 2014;Klink & Thibaudeau 2014).…”

Section: Introductionmentioning

confidence: 99%

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A plant transformation system designed for high throughput genomics inGossypium hirsutumto study root–organism interactions

Pant

McNeece

Sharma

et al. 2015

Journal of Plant Interactions

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The study of biological processes has been aided greatly by the development of procedures to identify the large numbers of associated genes. However, the ability to study the identified genes experimentally is often impeded by the absence of technologies to perform such functional analyses. Here, a nonaxenic plant transformation system has been developed in Gossypium hirsutum (cotton) for the study of genes associated with root functions and root-organism interactions. The plant transformation system is compatible with modern high throughput plant transformation goals and the processing of large numbers of genes intended for the study of root function in G. hirsutum.

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Section: Prap15 Plasmidmentioning

confidence: 99%

Section: Prap15 Plasmidmentioning

confidence: 99%

Section: Prap15 Plasmidmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A plant transformation system designed for high throughput genomics inGossypium hirsutumto study root–organism interactions

Pant

McNeece

Sharma

et al. 2015

Journal of Plant Interactions

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Characterizing resistance to soybean cyst nematode in PI 494182, an early maturing soybean accession

et al. 2020

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The soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) generates more damage to soybean [Glycine max (L.) Merr.] than any other parasite in most soybeanproducing countries. The use of SCN-resistant cultivars remains the most effective method to limit losses caused by SCN. The SCN-resistant accession PI 88788 has been used almost exclusively to control SCN over the past decades, inducing a shift in nematode virulence to overcome the resistance. Furthermore, PI 88788 and other sources of resistance characterized to date belong to maturity groups (MGs) III and higher, making them less attractive to develop early maturing soybean varieties (MGs 0-000). In this work, we performed a quantitative trait loci (QTL) analysis of the SCNresistant soybean accession PI 494182 (MG 0). A recombinant inbred lines (RILs) population ('Costaud' × PI 494182) segregating for SCN resistance was challenged with SCN (H. glycines [HG] type 0) and genotyped via genotyping-by-sequencing (GBS) to produce a genetic map. Six resistance QTL were identified, including a potentially new resistance locus on chromosome 07. A subset of the RIL population was confronted to a HG type 2.5.7 SCN population and some of these exhibited resistance toward this type. Whole-genome sequencing of PI 494182 and Costaud allowed us to determine the alleles and their copy number for three candidate genes: GmSNAP11, GmSNAP18 (Rhg1), and GmSHMT08 (Rhg4). Finally, we determined that selecting for PI 494182 alleles at some SCN-resistance QTL could entail linkage drag (decrease in protein concentration and 100-seed weight, increase in oil concentration). This work provides useful markers for introgressing SCN resistance in early maturing soybean varieties.

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The rhg1‐a (Rhg1 low‐copy) nematode resistance source harbors a copia‐family retrotransposon within the Rhg1‐encoded α‐SNAP gene

et al. 2019

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Soybean growers widely use the R esistance to H eterodera g lycines 1 ( Rhg1 ) locus to reduce yield losses caused by soybean cyst nematode (SCN). Rhg1 is a tandemly repeated four gene block. Two classes of SCN resistance‐conferring Rhg1 haplotypes are recognized: rhg1‐a (“Peking‐type,” low‐copy number, three or fewer Rhg1 repeats) and rhg1‐b (“PI 88788‐type,” high‐copy number, four or more Rhg1 repeats). The rhg1‐a and rhg1‐b haplotypes encode α‐SNAP (alpha‐ S oluble N SF A ttachment P rotein) variants α‐SNAP Rhg1 LC and α‐SNAP Rhg1 HC, respectively, with differing atypical C‐terminal domains, that contribute to SCN resistance. Here we report that rhg1‐a soybean accessions harbor a copia retrotransposon within their Rhg1 Glyma.18G022500 (α‐SNAP‐encoding) gene. We termed this retrotransposon “ RAC, ” for R hg1 a lpha‐SNAP c opia. Soybean carries multiple RAC ‐like retrotransposon sequences. The Rhg1 RAC insertion is in the Glyma.18G022500 genes of all true rhg1‐a haplotypes we tested and was not detected in any examined rhg1‐b or Rhg1 WT (single‐copy) soybeans. RAC is an intact element residing within intron 1, anti‐sense to the rhg1‐a α‐SNAP open reading frame. RAC has intrinsic promoter activities, but overt impacts of RAC on transgenic α‐SNAP Rhg1 LC mRNA and protein abundance were not detected. From the native rhg1‐a RAC + genomic context, elevated α‐SNAP Rhg1 LC protein abundance was observed in syncytium cells, as was previously observed for α‐SNAP Rhg1 HC (whose rhg1‐b does not carry RAC ). Using a SoySNP50K SNP corresponding with RAC presence, just ~42% of USDA accessions bearing previously identified rhg1‐a SoySNP50K SNP signatures harbor the RAC insertion. Subsequent analysis of several of these putative rhg1‐a accessions lacking RAC revealed that none encoded α‐SNAP Rhg1 LC , and thus, they are not rhg1‐a . ...

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The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection

Cited by 68 publications

References 149 publications

A plant transformation system designed for high throughput genomics inGossypium hirsutumto study root–organism interactions

A plant transformation system designed for high throughput genomics inGossypium hirsutumto study root–organism interactions

Characterizing resistance to soybean cyst nematode in PI 494182, an early maturing soybean accession

The rhg1‐a (Rhg1 low‐copy) nematode resistance source harbors a copia‐family retrotransposon within the Rhg1‐encoded α‐SNAP gene

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