The Expression Levels and Significance of GSH, MDA, SOD, and 8-OHdG in Osteochondral Defects of Rabbit Knee Joints

Abstract

Objective. To observe the dynamic changes in oxidative stress and the expression levels of antioxidant and oxidative parameters in the blood and synovial fluid in the osteochondral defects of the rabbit knee joints and to explore the significance. Methods. Thirty New Zealand white rabbits were randomly selected and divided into a blank control group ( n = 10 ), a model control group ( n = 10 ), and an osteochondral defect group ( n = 10 ). The osteochondral defect model of rabbit knee joint was constructed by medial parapatellar arthrotomy. The expression levels of glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and 8-hydroxydeoxyguanosine (8-OHdG) in peripheral venous blood and knee synovial fluid of the three groups were measured at the end of the 4th, 8th, and 12th weeks after treatment. Results. The expression levels of GSH and SOD in the blood and synovial fluid in the osteochondral defect group at the end of the 8th and 12th weeks were observably lower than those of the other two groups ( P < 0.05 ); higher expression levels of MDA and 8-OHdG in the blood and synovial fluid of the osteochondral defect group compared with those of the other two groups were obtained ( P < 0.05 ). At the end of the 4th, 8th, and 12th weeks, the expression levels of MDA and 8-OHdG in the blood and synovial fluid of the osteochondral defect group presented an upward trend ( P < 0.05 ). Conclusion. The osteochondral defects initiate the oxidative stress in the body, which is presented as the decrease of GSH and SOD expression, and the upregulation of MDA and 8-OHdG expression.

Mechanism of 2,4‐Dichlorophenoxyacetic acid‐induced damage to rat testis via Fas/FasL pathway and the protective effect of Lycium barbarum polysaccharides

“…SOD and GSH-Px are important markers for the antioxidant capacity of the body. 44 There was oxidative stress in the testis of mice exposed to 2,4-D, resulting in the decrease of SOD activity and the increase of MDA content in serum. 45 Our results showed that the serum content of MDA in 2,4-D-exposed rats increased, while the activities of SOD and GSH-Px decreased, which indicated that the antioxidant ability was weakened and the lipid peroxidation was enhanced, which caused the body to enter a state of oxidative stress, potentially affecting the spermatogenic function in rat testis.…”

“…As an end product of lipid oxidation, MDA reflects the damage of lipid peroxidation through the cell membrane. SOD and GSH‐Px are important markers for the antioxidant capacity of the body 44 . There was oxidative stress in the testis of mice exposed to 2,4‐D, resulting in the decrease of SOD activity and the increase of MDA content in serum 45 .…”

Effects of three feed attractants on the growth performance and meat quality of the largemouth bass (Micropterus salmoides)

“…GSH is the most prominent non-enzymatic antioxidant in fish, as well as a free radical scavenger and detoxifier (Chen et al, 2015). MDA, a byproduct of lipid peroxidation that can interact with the free amino groups in protein causing cell damage (Xiao et al, 2022), and the contents of MDA in the fish liver can reflect the severity of the free radical attack on the liver or body cells (Całyniuk et al, 2016). Previous studies have demonstrated that limonene, allicin and betaine could improve the antioxidant capacity of fish (Abdel-Tawwab et al, 2021;Dong et al, 2021;Hamed et al, 2021;Ajiboye et al, 2016;Lopes et al, 2019;Lopes et al, 2020;Mohseni et al, 2021).…”

Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice

“…SOD, MDA, and GSH are the main biomarkers of oxidative stress [ 41 ]. SOD is an important antioxidant enzyme that can remove free radicals in the body, especially superoxide anions [ 42 ].…”