The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis

Li, Wanjuan; Xue, Shenglun; Pang, Min; Yue, Zonghao; Yang, Deyong; Zhou, Yan; Zhao, Huan

doi:10.1007/s00343-019-7304-0

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…Finally, the maximum phosphate concentration in our experiment was well within that of the seawater in the natural habitat of P. aibuhitensis (0.087-0.968 mg L -1 ) (Zhang et al, 2018). Several other studies have shown that P. aibuhitensis has a strong tolerance for various poisonous chemicals (Li et al, 2018a;Li et al, 2018b). Thus, the results show that it is unlikely that P. aibuhitensis pumped water with the purpose to remove harmful solutes from the burrow, it is possible that the removal of harmful solutes is a secondary result from water pumping.…”

Section: Discussionmentioning

confidence: 58%

Why and How is Burrow Ventilation Initiated? A Case Study of Polychaete Behavior in the Burrow at Different Temperatures

Fang

Meng²,

Lux³

et al. 2022

Front. Mar. Sci.

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Bioirrigation is a process corresponding to animal-induced transport of porewater solute or exchange between interstitial and overlying waters. How and why the burrowing macrobenthos complete this process remain unclear. Here, we used two infaunal-behavior observation devices to investigate the ventilation behaviors of the benthic polychaete Perinereis aibuhitensis Grube, a species that is widespread along the Asian coast with a wide temperature range, at various temperatures (5°C, 10°C, 15°C, 20°C, and 25°C). The two typical movement behaviors of P. aibuhitensis, axial crawling and radial undulation, as well as associated parameters, were delimited and quantified. We found that the radial undulation frequency of P. aibuhitensis and the associated pumping rate (PR) increased significantly with temperature (T, 5-25°C); this relationship can be described by the regression equation PR = −0.0067T2 + 0.29T - 0.52 (R2 = 0.749, P < 0.05; n = 15). The relationship between axial crawling velocity (ACV) and temperature (T, 5-25°C) can be expressed by the regression equation ACV = -0.0001T3 + 0.0059T2 - 0.063T + 0.28 (R2 = 0.997, P<0.05; n = 15). In general, pumping efficiency increased as temperature decreased, implying that the polychaete increasingly conserved energy at lower temperatures. Peak pumping volume (4.36 L d-1) was observed at 25°C, as a result of radial undulations. Thus, we concluded that radial undulation was the primary movement that led to bioirrigation. The dissolved oxygen demand was the key factor driving the initiation of radial undulation, and the specific aim of radial undulation was to increase oxygen availability by pumping new seawater into the burrow. Thus, radial undulation is critical for polychaete survival. The dissolved oxygen threshold level at which pumping was initiated increased with temperature, suggesting more energy conserved at lower temperatures. This pumping strategy of P. aibuhitensis is consistent with optimality theory, and is here designated the “optimal dissolved oxygen obtainment strategy”.

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Section: Discussionmentioning

confidence: 58%

Why and How is Burrow Ventilation Initiated? A Case Study of Polychaete Behavior in the Burrow at Different Temperatures

Fang

Meng²,

Lux³

et al. 2022

Front. Mar. Sci.

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“…Real-time fluorescence quantitative PCR was used to investigate the expression of the two genes in P. aibuhitensis during B(a)P exposure. The β-actin gene was used as the reference gene, according to our previous study (Li et al, 2018). The primer information is shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure

Huang

Sun

Han

et al. 2019

PeerJ

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BackgroundG-protein-coupled receptors (GPCRs) are one of the most important molecules that transfer signals across the plasma membrane, and play central roles in physiological systems. The molecular architecture of GPCRs allows them to bind to diverse chemicals, including environmental contaminants.MethodsTo investigate the effects of benzo(a)pyrene (B(a)P) on GPCR signaling, GPCR and the protein kinase A (PKA) catalytic subunit of Perinereis aibuhitensis were cloned. The expression patterns of these two genes during B(a)P exposure were determined with real-time fluorescence quantitative PCR. The PKA content in P. aibuhitensis under B(a)P exposure was examined.ResultsThe full-length cDNAs of PaGPCR and the PaPKA catalytic subunit were 1,514 and 2,662 nucleotides, respectively, encoding 338 and 350 amino acids, respectively. Multiple sequence alignments indicated that the deduced amino acid sequence of PaGPCR shared a low level of similarity with the orphan GPCRs of polychaetes and echinoderms, whereas PaPKA shared a high level of identify with the PKA catalytic subunits of other invertebrates. B(a)P exposure time-dependently elevated the expression of PaGPCR and PaPKA. The expression of both PaGPCR and PaPKA was also dose-dependent, except at a dose of 10 μg/L B(a)P. The PKA content in concentration group was elevated on day 4, with time prolonging the PKA content was down-regulated to control level.DiscussionThese results suggested that GPCR signaling in P. aibuhitensis was involved in the polychaete’s response to environmental contaminants.

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Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

Onyena,

Manohar,

Nkwoji

et al. 2023

Aquat Ecol

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The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis

Cited by 5 publications

References 35 publications

Why and How is Burrow Ventilation Initiated? A Case Study of Polychaete Behavior in the Burrow at Different Temperatures

Why and How is Burrow Ventilation Initiated? A Case Study of Polychaete Behavior in the Burrow at Different Temperatures

Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure

Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

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