The expression and significance of survivin mRNA in urinary bladder carcinomas

Wang, Haidong; Xi, Xiaoqing; Kong, Xianguo; Huang, Guohua; Ge, Gen

doi:10.1007/s00432-004-0561-z

Cited by 46 publications

(38 citation statements)

References 9 publications

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“…2). Although survivin mRNA has been detected in various human cancers, 29,30) the lack of statistical significance in our 22 NB cases is possibly due to the low number of patients who died (6) compared with those who survived (12).…”

Section: Resultsmentioning

confidence: 67%

Analyses of Novel Prognostic Factors in Neuroblastoma Patients

Ootsuka

Asami

Sasaki

et al. 2007

Biological & Pharmaceutical Bulletin

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Section: Resultsmentioning

confidence: 67%

Analyses of Novel Prognostic Factors in Neuroblastoma Patients

Ootsuka

Asami

Sasaki

et al. 2007

Biological & Pharmaceutical Bulletin

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“…29 Moreover, promising results have been obtained when using a nested PCR assay for survivin mRNA detection in urine specimens of bladder carcinoma patients. 30 Our report is the first to quantify survivin mRNA expression in both exfoliated urinary cells and corresponding tumor tissues. In our study, no correlation could be demonstrated between tissue and urinary survivin levels.…”

Section: Discussionmentioning

confidence: 90%

Quantitative analysis of survivin mRNA expression in urine and tumor tissue of bladder cancer patients and its potential relevance for disease detection and prognosis

Weikert

Christoph

Schrader

et al. 2005

Intl Journal of Cancer

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Suppression of apoptosis may favor the onset and progression of cancer. Survivin is an inhibitor of apoptosis that has been suggested as a novel diagnostic/prognostic marker of bladder cancer. In this study, survivin mRNA expression was measured by a sensitive real-time PCR assay in tumor tissue and urine from bladder cancer patients and assessed for its potential diagnostic and prognostic relevance. Specimens from 53 patients with bladder transitional cell carcinoma (TCC) were analyzed, the controls being normal urothelial tissues (n = 14) and urine from benign disease patients (n = 22) and healthy individuals (n = 14). Survivin transcripts were commonly detected in tumor tissues, but not in normal urothelium, and increasing mRNA levels correlate with progressing pathologic stage (p = 0.001) and grade categories (p < 0.004). Higher levels of expression were associated with a reduced time to recurrence in noninvasive TCCs (p = 0.027, logrank test) and a trend toward shorter disease-free survival in muscle-invasive tumors (p = 0.067). Urinary survivin analysis detects TCC with higher sensitivity (68.6%) and equal specificity (100%) when compared with cytology (31.4% and 97.1%). Our results indicate that tissue levels of survivin mRNA predict disease-free survival in noninvasive TCC and may have a role in bladder cancer progression. When analyzed by RT-PCR in urine, survivin is a highly specific biomarker for TCC detection. ' 2005 Wiley-Liss, Inc.

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“…Recently, it has been proposed as a new marker for bladder cancer detection (34 -36). Urine detection of survivin is useful for the diagnosis and prognosis in bladder carcinomas (35,36). It is correlated with decreased survival, unfavorable prognosis, and accelerated rates of recurrences in cancer therapy (37).…”

Section: Introductionmentioning

confidence: 99%

Baicalein induces cancer cell death and proliferation retardation by the inhibition of CDC2 kinase and survivin associated with opposite role of p38 mitogen-activated protein kinase and AKT

Liu

2007

Molecular Cancer Therapeutics

127

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The bioactive flavonoid baicalein has been shown to have in vitro growth-inhibitory activity in human cancer cells, although the mechanism of action is poorly understood. Baicalein (40 -80 Mmol/L for 24 h) more effectively induced cytotoxicity compared with other flavonoids (baicalin, catechin, genistein, quercetin, and rutin) in bladder cancer cells. Baicalein induced cell proliferation inhibition and apoptosis. The levels of cyclin B1 and phospho-CDC2 (Thr 161 ) were reduced, whereas the G 2 -M phases were elevated by baicalein. Treatment of CDC2 kinase or CDC25 phosphatase inhibitors augments the baicalein-induced cytotoxicity. A variety of human bladder cancer cell lines expressed survivin proteins, which were located on the mitotic phases and regulated mitotic progression. Baicalein markedly reduced survivin protein expression. Transfection of a survivin small interfering RNA diminished the level of survivin proteins and increased the baicalein-mediated cell death. Overexpression of survivin enhanced cell proliferation and resisted the baicalein-induced cytotoxicity. Interestingly, baicalein induced the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and AKT. SB203580, a specific p38 MAPK inhibitor, attenuated proliferation inhibition and restored the protein levels of phospho-CDC2 (Thr 161 ) and survivin in the baicalein-exposed cells; conversely, blockade of AKT activation enhanced cytotoxicity and the reduction of phospho-CDC2 (Thr 161 ) and survivin proteins.As a whole, these findings provide that the opposite role of p38 MAPK and AKT regulates CDC2 kinase and survivin and the inhibition of CDC2-survivin pathway by baicalein contributes to apoptosis and proliferation retardation in cancer cells.

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The expression and significance of survivin mRNA in urinary bladder carcinomas

Cited by 46 publications

References 9 publications

Analyses of Novel Prognostic Factors in Neuroblastoma Patients

Analyses of Novel Prognostic Factors in Neuroblastoma Patients

Quantitative analysis of survivin mRNA expression in urine and tumor tissue of bladder cancer patients and its potential relevance for disease detection and prognosis

Baicalein induces cancer cell death and proliferation retardation by the inhibition of CDC2 kinase and survivin associated with opposite role of p38 mitogen-activated protein kinase and AKT

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