The expression and role of serum response factor in papillary carcinoma of the thyroid

Moon,

doi:10.3892/ijo_00000312

Cited by 6 publications

(1 citation statement)

References 16 publications

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“…MRTF‐A or SRF depletion could reduce the metastasis of both human breast cancer cells and mouse melanoma cells (Medjkane et al, 2009). Overexpression of SRF enhanced the cell motility and invasiveness of hepatocellular carcinoma cells and papillary carcinoma cells (Kim et al, 2009; Kwon et al, 2010). Yin et al also found that SRF was highly expressed in gastric cancer, and disease‐free survival rate and overall survival rate were both significantly lower in patients with high SRF expression (Yin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

MRTF‐A/SRF signaling suppresses invasion of oral squamous cell carcinoma

Yang

et al. 2023

Oral Diseases

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BackgroundSerum response factor (SRF) and myocardial‐associated transcription factor‐A (MRTF‐A) had different regulatory effects on the tumorigenesis and development in different cancers. However, the role of MRTF‐A/SRF in oral squamous cell carcinoma (OSCC) remains to be determined.MethodsCCK‐8 assay, cell scratch experiment, and transwell invasion assay were conducted to investigate the effects of MRTF‐A/SRF on biological behavior of OSCC cells. The expression pattern and prognostic value of MRTF‐A/SRF in OSCC were analyzed based on cBioPortal website and TCGA database. Protein–protein interaction network was visualized to identify protein functions. Go and KEGG pathway analyses were performed to investigate related pathways. The effect of MRTF‐A/SRF on epithelial–mesenchymal transformation (EMT) of OSCC cells was explored by western blot assay.ResultsOverexpression of MRTF‐A/SRF inhibited the proliferation, migration, and invasion of OSCC cells in vitro. High expression of SRF was related to better prognosis of OSCC patients on hard palate, alveolar ridge, and oral tongue. Besides, overexpression of MRTF‐A/SRF inhibited the EMT of OSCC cells.ConclusionSRF was closely related to the prognosis of OSCC. High expression of SRF and its co‐activator MRTF‐A inhibited proliferation, migration, and invasion of OSCC cells in vitro, possibly via EMT suppression.

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Section: Introductionmentioning

confidence: 99%

MRTF‐A/SRF signaling suppresses invasion of oral squamous cell carcinoma

Yang

et al. 2023

Oral Diseases

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Novel genetic variants in differentiated thyroid cancer and assessment of the cumulative risk

Figlioli

Chen

Elisei

et al. 2015

Sci Rep

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A genome-wide association study (GWAS) performed on a high-incidence Italian population followed by replications on low-incidence cohorts suggested a strong association of differentiated thyroid cancer (DTC) with single nucleotide polymorphisms (SNPs) at 9q22.33, 2q35, 20q11.22-q12 and 14q24.3. Moreover, six additional susceptibility loci were associated with the disease only among Italians. The present study had two aims, first to identify loci involved in DTC risk and then to assess the cumulative effect of the SNPs identified so far in the Italian population. The combined analysis of the previous GWAS and the present Italian study provided evidence of association with rs7935113 (GALNTL4, OR = 1.36, 95%CI 1.20–1.53, p-value = 7.41 × 10−7) and rs1203952 (FOXA2, OR = 1.29, 95%CI 1.16–1.44, p-value = 4.42 × 10−6). Experimental ENCODE and eQTL data suggested that both SNPs may influence the closest genes expression through a differential recruitment of transcription factors. The assessment of the cumulative risk of eleven SNPs showed that DTC risk increases with an increasing number of risk alleles (p-trend = 3.13 × 10−47). Nonetheless, only a small fraction (about 4% on the disease liability scale) of DTC is explained by these SNPs. These data are consistent with a polygenic model of DTC predisposition and highlight the importance of association studies in the discovery of the disease hereditability.

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Clinical Significance and Potential Regulatory Mechanisms of Serum Response Factor in 1118 Cases of Thyroid Cancer Based on Gene Chip and RNA-Sequencing Data

et al. 2020

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Departmental sources Background:Thyroid cancer (TC) is one of the most prevalent endocrine malignancies and there may be many unclarified molecular events and gene types involved in TC. The objective of this study was to assess the clinical implications and potential mechanisms of serum response factor (SRF) in TC. Material/Methods:RNA-sequencing and gene chip data with TC expression were collected from The Cancer Genome Atlas/Genotype-Tissue Expression, Gene Expression Omnibus, ArrayExpress, Sequence Read Archive, and Oncomine. SRF expression of all TC and adjacent non-cancerous tissue were calculated using the t test, STATA, and Meta-DiSc.The related pathways of the potential SRF target genes and target miRNAs were explored. Dual-luciferase reporter assay was performed to validate the association between SRF and its putative miRNA. Results:One RNA-sequencing and 15 gene chips were collected, and the pooled standardized mean difference of SRF was -1.00. Furthermore, the area under the curve of sROC of SRF in TC was 0.8251, indicating a dramatic decreased expression of SRF in TC tissues based on 1118 cases. The intersection of differentially expressed genes in TC, SRF co-expressed genes, and SRF potential target genes achieved from Cistrome Cancer led to 169 overlapped genes. miR-330-5p was predicted to target SRF, which was further confirmed by dual-luciferase reporter assay. Conclusions:The reduction of SRF appears to play a crucial role in the origin of TC. These properties are accomplished by the target genes of SRF, as a transcription factor, or by the axes with the associated miRNAs.

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The expression and role of serum response factor in papillary carcinoma of the thyroid

Cited by 6 publications

References 16 publications

MRTF‐A/SRF signaling suppresses invasion of oral squamous cell carcinoma

MRTF‐A/SRF signaling suppresses invasion of oral squamous cell carcinoma

Novel genetic variants in differentiated thyroid cancer and assessment of the cumulative risk

Clinical Significance and Potential Regulatory Mechanisms of Serum Response Factor in 1118 Cases of Thyroid Cancer Based on Gene Chip and RNA-Sequencing Data

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