The expression and methylation of PITX genes is associated with the prognosis of head and neck squamous cell carcinoma

Zhao, Ya‐Qiong; Zhao, Jie; Zhong, Meng-Mei; Zhang, Qian; Yan, Fei; Feng, Yun‐Zhi; Guo, Yue

doi:10.3389/fgene.2022.982241

Cited by 3 publications

(2 citation statements)

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“…The heatmap displayed the distribution of clinical data for two different expression groups of TRIB3. Furthermore, TRIB3 expression was significantly correlated with the T-stage, and patients with higher T-stage had significantly higher TRIB3 expression than lower T-stage patients ( Figure 4E ), which suggests that TRIB3 expression is related to tumor growth and infiltration ability ( 33 ).…”

Section: Resultsmentioning

confidence: 97%

TRIB3, as a robust prognostic biomarker for HNSC, is associated with poor immune infiltration and cancer cell immune evasion

Wu,

Fu,

et al. 2024

Front. Immunol.

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ObjectiveAs a pseudokinase, Tribbles Pseudokinase 3 (TRIB3) is implicated in a wide array of biological processes, including cell signal transduction, metabolic regulation, stress responses, and immune regulation. While its significant role in the immune regulation of certain cancers is well-established, the specific functions and impact of TRIB3 in head and neck squamous cell carcinoma (HNSC) remain unclear.MethodsThe data of RNA-sequence was acquired from the TCGA database to analyze the expression patterns of TRIB3 and elucidate its prognostic value in HNSC patients. Furthermore, the correlation between TRIB3 and tumor mutation burden, clinical data, immune checkpoint genes, and immune cell infiltration was explored. Moreover, the TRIB3 location in tumor tissues and subcellular structures was identified via Tisch in the HPA database, and the potential protein interaction molecules for TRIB3 were elucidated in the STRING database. The potential TRIB3 gene function was assessed using gene set enrichment analysis (GSEA), whereas the TRIB3 expression levels in clinical HNSC samples were verified by RT-qPCR and immunohistochemistry. the role of TRIB3 in enhancing the malignant behavior of HNSC cells was validated in vitro through a series of methods including RT-qPCR, CCK8 assay, wound healing assay, and transwell assay.ResultsIt was revealed that TRIB3 was significantly overexpressed in the nucleus and cytoplasm of HNSC. Furthermore, this overexpression markedly enhanced the migration ability of tumor cells. As an independent prognostic factor, TRIB3 was associated with advanced tumor T stage and was significantly involved with tumor mutation burden and immune cell infiltration in HNSC. Moreover, it was observed that TRIB3 was not a predicted factor for PD1/PDL1 and ATL4 inhibitor treatment; however, it was substantially correlated with various immune evasion-related genes in HNSC.ConclusionTRIB3 could serve as a potential prognostic marker for HNSC and might be a key gene mediating HNSC immune evasion.

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Section: Resultsmentioning

confidence: 97%

TRIB3, as a robust prognostic biomarker for HNSC, is associated with poor immune infiltration and cancer cell immune evasion

Wu,

Fu,

et al. 2024

Front. Immunol.

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“…This reduction might be linked to PITX1 hypermethylation. Additionally, patients with elevated PITX1 expression experienced worse stage, grade, and relapse-free survival (RFS) which was an opposing result of Takenobu et al The databases also suggested correlations between PITX1 and immune cell expression, including CD4+ and CD8+ T cells (25,40). However, relying solely on public databases might limit the accuracy of these predictions.…”

Section: Head and Neck Squamous Cell Carcinomamentioning

confidence: 99%

PITX1 plays essential functions in cancer

Zhao,

2023

Front. Oncol.

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PITX1, also known as the pituitary homeobox 1 gene, has emerged as a key regulator in animal growth and development, attracting significant research attention. Recent investigations have revealed the implication of dysregulated PITX1 expression in tumorigenesis, highlighting its involvement in cancer development. Notably, PITX1 interacts with p53 and exerts control over crucial cellular processes including cell cycle progression, apoptosis, and chemotherapy resistance. Its influence extends to various tumors, such as esophageal, colorectal, gastric, and liver cancer, contributing to tumor progression and metastasis. Despite its significance, a comprehensive review examining PITX1’s role in oncology remains lacking. This review aims to address this gap by providing a comprehensive overview of PITX1 in different cancer types, with a particular focus on its clinicopathological significance.

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Identifying biomarkers associated with the diagnosis of ulcerative colitis via bioinformatics and machine learning

Wang

Huang

Zhang

et al. 2023

MBE

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<abstract> <sec><title>Background</title>Ulcerative colitis (UC) is an idiopathic inflammatory disease with an increasing incidence. This study aimed to identify potential UC biomarkers and associated immune infiltration characteristics. </sec> <sec><title>Methods</title>Two datasets (GSE87473 and GSE92415) were merged to obtain 193 UC samples and 42 normal samples. Using R, differentially expressed genes (DEGs) between UC and normal samples were filtered out, and their biological functions were investigated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Promising biomarkers were identified using least absolute shrinkage selector operator regression and support vector machine recursive feature elimination, and their diagnostic efficacy was evaluated through receiver operating characteristic (ROC) curves. Finally, CIBERSORT was used to investigate the immune infiltration characteristics in UC, and the relationship between the identified biomarkers and various immune cells was examined. </sec> <sec><title>Results</title>We found 102 DEGs, of which 64 were significantly upregulated, and 38 were significantly downregulated. The DEGs were enriched in pathways associated with interleukin-17, cytokine–cytokine receptor interaction and viral protein interactions with cytokines and cytokine receptors, among others. Using machine learning methods and ROC tests, we confirmed DUOX2, DMBT1, CYP2B7P, PITX2 and DEFB1 to be essential diagnostic genes for UC. Immune cell infiltration analysis revealed that all five diagnostic genes were correlated with regulatory T cells, CD8 T cells, activated and resting memory CD4 T cells, activated natural killer cells, neutrophils, activated and resting mast cells, activated and resting dendritic cells and M0, M1 and M2 macrophages. </sec> <sec><title>Conclusions</title>DUOX2, DMBT1, CYP2B7P, PITX2 and DEFB1 were identified as prospective biomarkers for UC. A new perspective on understanding the progression of UC may be provided by these biomarkers and their relationship with immune cell infiltration. </sec> </abstract>

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The expression and methylation of PITX genes is associated with the prognosis of head and neck squamous cell carcinoma

Cited by 3 publications

References 48 publications

TRIB3, as a robust prognostic biomarker for HNSC, is associated with poor immune infiltration and cancer cell immune evasion

TRIB3, as a robust prognostic biomarker for HNSC, is associated with poor immune infiltration and cancer cell immune evasion

PITX1 plays essential functions in cancer

Identifying biomarkers associated with the diagnosis of ulcerative colitis via bioinformatics and machine learning

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