The expression and clinical significance of miR-30b-3p and miR-125b-1-3p in patients with periodontitis

Abstract

Objective Periodontitis is a chronic inflammatory infectious disease caused by the deposition of dental plaque on the tooth surface, leading to adverse systemic consequences. Accumulating evidence shows that dysregulated microRNAs (miRNAs) are associated with the disease severity of periodontitis. Herein, we report two novel miRNAs, miR-30b-3p and miR-125b-1-3p, in the context of periodontitis and their relationships with disease severity of periodontitis. Methods The miRNA profiles of gingival crevicular fluid (GCF) samples were used to screen differentially expressed miRNAs (DEmiRNAs) between periodontitis patients and periodontally healthy individuals. Clinical human GCF samples were collected from 80 patients diagnosed with periodontitis (PD +) for the first time and 100 periodontally healthy individuals (PD-). The severity of periodontitis was categorized into mild/moderate (MPD) and severe (SPD) groups. The expressions of miR-30b-3p and miR-125b-1-3p were determined by quantitative real-time PCR. The levels of IL-1β, IL-6, and TNF-α were determined by ELISA methods. Results We applied GEO2R bioinformatics tool to analyze the raw data of the GSE89081 dataset and identified miR-30b-3p (|logFC|= 1.987) and miR-125b-1-3p (|logFC|= 1.878) between periodontitis patients and periodontally healthy individuals. It was found that PPD, CAL, BOP, and the relative expression levels of miR-30b-3p and miR-125b-1-3p were all higher in the PD + group than the PD- group, in the SPD group than the MPD group (P < 0.05). The periodontitis patients with high-miR-30b-3p expression exhibited increased PPD, CAL, and BOP compared to those low-miR-30b-3p expression, while high-miR-125b-1-3p expression group showed significant differences on PPD and BOP from low-miR-125b-1-3p expression group (P < 0.05). Pearson correlation analysis demonstrated a significantly positive correlation between the levels of inflammatory cytokines, miR-30b-3p expression, and miR-125b-1-3p expression (P < 0.001). Results of ROC curves showed AUC of 0.878 and 0.927, sensitivity of 0.843 and 0.855, and specificity of 0.791 and 0.801, respectively, when miR-30b-3p and miR-125b-1-3p expression levels were used to diagnose periodontitis. Conclusion These data unveiled that miR-30b-3p and miR-125b-1-3p expressions may be associated with the pathogenesis of periodontitis.

MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis

“…Some authors have analyzed the expression level of several miRNAs in GCF, but none have carried out a complete screening of all the miRNAs [44][45][46][47][48]. The results of this study agree with the results of Rovas et al, 2022 and Zhu and Zhong, 2022, reporting high expression level of miRNAs miR-30a and miR-146a in periodontitis [45,47].…”

“…Saito et al employed a ready-to-use PCR with 720 miRNAs, while Radovic et al analyzed the expression levels of two miRNAs before and after periodontal treatment in patients with periodontitis and diabetes, reporting that the expression levels of miR-146a-5p and miR-155 decreased after treatment [36,43]. Some authors have analyzed the expression level of several miRNAs in GCF, but none have carried out a complete screening of all the miRNAs [44][45][46][47][48]. The results of this study agree with the results of Rovas et al, 2022 and Zhu and Zhong, 2022, reporting high expression level of miRNAs miR-30a and miR-146a in periodontitis [45,47].…”

Diagnostic potential of miR-200 family members in gingival crevicular fluid for chronic periodontitis: correlation with clinical parameters and therapeutic implications

“…CP is a prevalent inflammatory disease that affects the supporting tissues of teeth, leading to tooth loss and a significant impact on patients’ quality of life [ 25 , 26 ]. Dysregulation of miRNA expression has been associated with various pathologies, making them excellent molecules to study as potential biomarkers for CP [ 19 , 27 ].…”

Blood miRNAs as Potential Diagnostic Biomarkers for Chronic Obstructive Pulmonary Disease: A Meta-Analysis

“…These criteria involved excluding reviews, in vitro and in vivo experiments, as well as articles with sample sources other than blood or those with unusable data. In conclusion, a total of 16 eligible studies [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] were incorporated into the meta-analysis, comprising 8 English-language publications and 8 Chinese-language publications. The publication period of the included studies spans from 2013 to 2023.…”