Abstract:Missed abortions are common complications that occur in early pregnancy, and impaired trophoblast functions have been indicated to be associated with their pathogenesis. The long noncoding RNAs (lncRNAs) Metastasis Associated Lung Adenocarcinoma Transcript 1 (
MALAT1)
, HOX Transcript Antisense RNA (
HOTAIR)
and Maternally expressed gene 3 (
MEG3)
have been demonstrated to serve a crucial regulatory role in the mobility of trophoblast cells a… Show more
“…Luo et al exhibited higher expression of HOTAIR in RSA patients compared with healthy controls 63 . Interestingly, it is reported that HOTAIR can be suppressed in RSA 64 .…”
Aim
To investigate the association between Hox transcript antisenses RNA (HOTAIR) polymorphisms, rs12826786 C/T, rs920778 T/C, rs4759314 A/G, and rs1899663 G/T, with recurrent spontaneous abortion (RSA) susceptibility in the Iranian women.
Methods
We enrolled 161 patients diagnosed with RSA and 177 healthy women with at least one live birth without a history of abortion. Genotyping of HOTAIR polymorphisms was carried out using both restriction fragment length polymorphism‐polymerase chain reaction and amplification refractory mutation system‐polymerase chain reaction methods. Odds ratios (ORs) with 95% confidence intervals (CIs) were assessed to estimate the strength of association.
Results
Different inheritance models of rs12826786 C/T, rs920778 T/C, and rs1899663 G/T polymorphisms significantly enhanced the risk of RSA (p < 0.05), whereas the rs4759314 A/G polymorphism was correlated with diminished risk of developing RSA under recessive AA versus GA + GG (OR 0.42 [95% CI = 0.19–0.91]), log‐additive GG versus GA vs. GG (OR 0.67 [95% CI = 0.48–0.93]), and allelic A versus G (OR 0.65 [95% CI = 0.47–0.92]) models. Moreover, the TGTC, TTCT, TTTC, CGTC, CGTT, CTCC, CTCT, CTTC, and CTTT haplotypes of rs920778/rs1899663/rs12826786/ significantly increased the risk of RSA. The studied variants were not in strong linkage disequilibrium.
Conclusions
Our results indicated that variations in the HOTAIR gene might serve as beneficial biomarkers for determining susceptibility to RSA. To confirm these findings, replication studies with a larger population and different races are needed.
“…Luo et al exhibited higher expression of HOTAIR in RSA patients compared with healthy controls 63 . Interestingly, it is reported that HOTAIR can be suppressed in RSA 64 .…”
Aim
To investigate the association between Hox transcript antisenses RNA (HOTAIR) polymorphisms, rs12826786 C/T, rs920778 T/C, rs4759314 A/G, and rs1899663 G/T, with recurrent spontaneous abortion (RSA) susceptibility in the Iranian women.
Methods
We enrolled 161 patients diagnosed with RSA and 177 healthy women with at least one live birth without a history of abortion. Genotyping of HOTAIR polymorphisms was carried out using both restriction fragment length polymorphism‐polymerase chain reaction and amplification refractory mutation system‐polymerase chain reaction methods. Odds ratios (ORs) with 95% confidence intervals (CIs) were assessed to estimate the strength of association.
Results
Different inheritance models of rs12826786 C/T, rs920778 T/C, and rs1899663 G/T polymorphisms significantly enhanced the risk of RSA (p < 0.05), whereas the rs4759314 A/G polymorphism was correlated with diminished risk of developing RSA under recessive AA versus GA + GG (OR 0.42 [95% CI = 0.19–0.91]), log‐additive GG versus GA vs. GG (OR 0.67 [95% CI = 0.48–0.93]), and allelic A versus G (OR 0.65 [95% CI = 0.47–0.92]) models. Moreover, the TGTC, TTCT, TTTC, CGTC, CGTT, CTCC, CTCT, CTTC, and CTTT haplotypes of rs920778/rs1899663/rs12826786/ significantly increased the risk of RSA. The studied variants were not in strong linkage disequilibrium.
Conclusions
Our results indicated that variations in the HOTAIR gene might serve as beneficial biomarkers for determining susceptibility to RSA. To confirm these findings, replication studies with a larger population and different races are needed.
“…Missed abortion is a special type of spontaneous abortion which accompany serious complication endanger the reproductive healthy [20]. Previous studies on missed abortion focused on chromosome abnormalities [21], uterine artery blood ow resistance, angiogenesis [22], immune in ammationrelated [23,24], etc. In recent years, with the further study of the human microbiome, researchers gradually realized that from the surface of human body to the intestinal ora, all of them have an impact on the ecological balance of human body and the occurrence and development of diseases.…”
BackgroundMissed abortion is a kind of pregnancy failure caused by various reasons. The etiology is complicated, and the incidence of miscarriage is increasing in recent years. Previous studies shown microbiota contributes to multi-systemic function, whereas the relationship between microbiota and early missed abortion remains unknown. This study aimed to explore the composition of uterine microbiota in missed abortion and the potential role.MethodsWe enrolled 19 patients diagnosed with missed abortion and 12 healthy pregnant who subsequently had 6-8th week pregnant. All samples were taken from the endometrial fluid by a special disposable endometrial sampler. After samples were collected, DNA was extracted and amplified. The high-throughput next-generation sequencing (MiSeq) of the 16S rDNA V3-V4 region was used to identify the present of microbiota. The α-diversity of microbiota data was used to reflect species richness and evenness within bacterial populations, β-diversity was used to reflect the shared diversity between bacterial populations, and Nonmetric Multidimensional Scaling based on Weighted Unifrac distance. Statistical was determined by use of multiple testing, including the generalized mixed-effects model.ResultsThe microbiome sequencing (16S rDNA V3-V4 region) revealed that low abundance microbiome was detected in uterine cavity of patients with missed abortion and normal pregnancy. The diversity of intrauterine microflora in patients with missed abortion was higher than that in patients with induced abortion in normal pregnancy. There was no significant difference in alpha diversity between the two groups, but a significant difference was observed in beta diversity. PCoA moment array analysis did not show significant differences. Proteobacteria and Firmicutes in missed abortion patients were significantly more than those in normal pregnancy group.ConclusionThere are low abundance of microflora in uterine cavity of missed abortion patients, and the diversity of microflora is higher than that of normal pregnancy patients. Also, Proteobacteria and Firmicutes may be potential biological markers in missed abortion. New observations may prompt further investigations to understand the potential mechanism of microbiology on pathologic human pregnancy in the future.SubjectsMicrobiology, Molecular Biology, Gynecology and Obstetrics, missed abortion, Female Reproductive Tract,
“…LncRNAs modulate a wide variety of biological processes, such as cellular differentiation, maintenance of stem cell pluripotency, and development of tissues and organs, contributing to the onset of human diseases [ 17 , 43 ]. They are implicated in different pregnancy-related complications, including preeclampsia [ 44 ], GDM [ 45 ], intrauterine growth restriction (IUGR) [ 46 ], and missed abortions [ 47 ].…”
In the era of personalized medicine, fetal sex-specific research is of utmost importance for comprehending the mechanisms governing pregnancy and pregnancy-related complications. In recent times, noncoding RNAs (ncRNAs) have gained increasing attention as critical players in gene regulation and disease pathogenesis, and as candidate biomarkers in human diseases as well. Different types of ncRNAs, including microRNAs (miRNAs), piwi-interacting RNAs (piRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), participate in every step of pregnancy progression, although studies taking into consideration fetal sex as a central variable are still limited. To date, most of the available data have been obtained investigating sex-specific placental miRNA expression. Several studies revealed that miRNAs regulate the (patho)-physiological processes in a sexually dimorphic manner, ensuring normal fetal development, successful pregnancy, and susceptibility to diseases. Moreover, the observation that ncRNA profiles differ according to cells, tissues, and developmental stages of pregnancy, along with the complex interactions among different types of ncRNAs in regulating gene expression, strongly indicates that more studies are needed to understand the role of sex-specific ncRNA in pregnancy and associated disorders.
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