The export of immunoglobulin D by human neoplastic B lymphocytes.

Abstract

An investigation has been made into the ability of human neoplastic B lymphocytes expressing surface IgM and IgD to export IgD in culture. Cells that expressed surface Ig of the lambda light chain type frequently exported IgD (10/12 patients), whereas cells expressing surface Ig of the kappa light chain type exported no IgD, although most (8/11 patients) were able to export IgM. It appears, therefore, that in most of the 23 cases studied, cells synthesizing IgD with lambda light chains can both express and export IgD, whereas those synthesizing IgD kappa can only insert it into the surface membrane. This finding and the known preponderance of lambda in plasma IgD imply that the possession of a lambda chain facilitates the IgD secretory pathway, a conclusion that implicates a control mechanism subsequent to the surface/secretory dichotomy arising from different splicings of heavy chain messenger RNA.

Lymphocytic lymphoma/B-chronic lymphocytic leukaemia – An immunohistopathological study of peripheral B lymphocyte neoplasia

“…Increased staining of PC with the monoclonal 33.1 which detects a distinct DC-related Class II molecule (Marti et al, 1983) (Cossman et al, 1983;Forbes et al, 1981;Maeda & Deegan, 1983;Okamura et al, 1982;Totterman et al, 1980Totterman et al, , 1981a. These effects represent, probably, quantitative changes rather than "differentiation" events, in that CLL cells can secrete IgM (Stevenson et al, 1980(Stevenson et al, , 1982, and monoclonal clg has been reported to be present in most cases of CLL (Gordon et al, 1983a;Guglielmi et al, 1982, Han et al, 1982Johnstone et al, 1982;Yasuda et al, 1982;Newell et al, 1983 Martin et al, 1981;Nanba et al, 1977). B-CLL cells are morphologically and phenotypically distinct from germinal centre cells .…”

Secretion of immunoglobulin by neoplastic B lymphocytes from lymph nodes of patients with lymphoma

“…Secretion of free monotypic light chain by the majority of CLL cell populations has also been observed (HannamHarris et al, 1980). A smaller proportion of such cell populations (8/19) all of which had surface Ig of the A light chain type, secreted monomeric IgD (Stevenson et al, 1982 (Stevenson et al, 1982); in NHL 23/24 secreted light chain and 17/241gM. For IgD, in CLL 8/19 were positive whereas in NHL 6/21 were positive.…”

“…Studies of such secretion have been carried out with neoplastic B lymphocytes from patients with chronic lymphocytic leukaemia (CLL) exporting IgD being of the A light chain type (Stevenson et al, 1982). For three of the cases, anti-idiotypic antibody was used to demonstrate the idiotypic nature of the exported Ig and for these patients it was shown that the low level of export led to an accumulation of idiotypic Ig in the plasma.…”

Consumption of monoclonal anti-idiotypic antibody by neoplastic B lymphocytes: A guide for immunotherapy

“…One of the major problems in the use of antiidiotypic antibody therapy is that most neoplastic B cell populations secrete small amounts of idiotypic Ig and this can accumulate in the plasma. This generalization appears to apply to the broad spectrum of human B cell neoplasms including chronic lymphocytic leukaemia and follicular lymphoma (Stevenson et al, 1982). Plasmapheresis can lower the plasma concentration by some 50-60% (Stevenson et al, 1980a).…”