The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing

Ratnawati, S.E.; Kuuliala, L.; Verschuere, N.; Cnockaert, M.; Vandamme, P.; Devlieghere, F.

doi:10.1016/j.fm.2023.104407

Cited by 2 publications

(2 citation statements)

References 96 publications

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“…Our workflow displayed that the use of these two methods without inaction step, and the entire process was conducted within a biosafety cabinet to mitigate the biosafety risks associated with the dispersal of fungal spores. Although there is no significant difference of identification accuracy between the MEK and the FA-sandwich method, the FA-sandwich benefits from its simple steps, rapid protein extraction without reagent cost and acetonitrile, and is more suitable for application in most clinical microbiology laboratories (Table 3 ) [ 29 ]. Traditionally, each filamentous fungi identified by the morphology of spore and hyphal required at least 5–10 days of simple cultured [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Wu,

Ao,

Zheng

et al. 2024

BMC Microbiol

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Objectives Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is extensively employed for the identification of filamentous fungi on MALDI Biotyper (Bruker Daltonics) and Vitek MS (biomerieux), but the performance of fungi identification on new EXS2600 (Zybio) is still unknow. Our study aims to evaluate the new EXS2600 system's (Zybio) ability to rapidly identify filamentous fungi and determine its effect on turnaround time (TAT) in our laboratory. Methods We tested 117 filamentous fungi using two pretreatment methods: the formic acid sandwich (FA-sandwich) and a commercial mold extraction kit (MEK, Zybio). All isolates were confirmed via sequence analysis. Laboratory data were extracted from our laboratory information system over two 9-month periods: pre-EXS (April to December 2022) and post-EXS (April to December 2023), respectively. Results The total correct identification (at the species, genus, or complex/group level) rate of fungi was high, FA-sandwich (95.73%, 112/117), followed by MEK (94.02%, 110/117). Excluding 6 isolates not in the database, species-level identification accuracy was 92.79% (103/111) for FA-sandwich and 91.89% (102/111) for MEK; genus-level accuracy was 97.29% (108/111) and 96.39% (107/111), respectively. Both methods attained a 100% correct identification rate for Aspergillus, Lichtheimia, Rhizopus Mucor and Talaromyces species, and were able to differentiate between Fusarium verticillioides and Fusarium proliferatum within the Fusarium fujikuroi species complex. Notably, high confidence was observed in the species-level identification of uncommon fungi such as Trichothecium roseum and Geotrichum candidum. The TAT for all positive cultures decreased from pre EXS2600 to post (108.379 VS 102.438, P < 0.05), and the TAT for tissue decreased most (451.538 VS 222.304, P < 0.001). Conclusions The FA-sandwich method is more efficient and accurate for identifying filamentous fungi with EXS2600 than the MEK. Our study firstly evaluated the performance of fungi identification on EXS2600 and showed it is suitable for clinical microbiology laboratories use.

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Section: Discussionmentioning

confidence: 99%

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Wu,

Ao,

Zheng

et al. 2024

BMC Microbiol

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“…Vibrio spp. Gram‐negative bacteria are defined by their curved and rod‐shaped morphology, preference for salty environments, ability to survive with or without oxygen, inability to form spores, movement aided by polar flagella and sheath, and positive reactivity to the oxidase test (Ratnawati et al., 2024). The bacteria are predominantly present in estuary and coastal habitats, where they inhabit water, sediments, plankton, and the various flora and fauna within coastal ecosystems (Landrigan et al., 2020).…”

Section: Microbiological Safety In Seafood Productsmentioning

confidence: 99%

Comprehensive analysis of predominant pathogenic bacteria and viruses in seafood products

Roy,

Jeon

et al. 2024

Comp Rev Food Sci Food Safe

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Given the growing global demand for seafood, it is imperative to conduct a comprehensive study on the prevalence and persistence patterns of pathogenic bacteria and viruses associated with specific seafood varieties. This assessment thoroughly examines the safety of seafood products, considering the diverse processing methods employed in the industry. The importance of understanding the behavior of foodborne pathogens, such as Salmonella typhimurium, Vibrio parahaemolyticus, Clostridium botulinum, Listeria monocytogenes, human norovirus, and hepatitis A virus, is emphasized by recent cases of gastroenteritis outbreaks linked to contaminated seafood. This analysis examines outbreaks linked to seafood in the United States and globally, with a particular emphasis on the health concerns posed by pathogenic bacteria and viruses to consumers. Ensuring the safety of seafood is crucial since it directly relates to consumer preferences on sustainability, food safety, provenance, and availability. The review focuses on assessing the frequency, growth, and durability of infections that arise during the processing of seafood. It utilizes next‐generation sequencing to identify the bacteria responsible for these illnesses. Additionally, it analyzes methods for preventing and intervening of infections while also considering the forthcoming challenges in ensuring the microbiological safety of seafood products. This evaluation emphasizes the significance of the seafood processing industry in promptly responding to evolving consumer preferences by offering current information on seafood hazards and future consumption patterns. To ensure the continuous safety and sustainable future of seafood products, it is crucial to identify and address possible threats.

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The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing

Cited by 2 publications

References 96 publications

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry

Comprehensive analysis of predominant pathogenic bacteria and viruses in seafood products

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