The Experimental Homologous and Heterologous Separation Distance Histograms for the Centromeres of Chromosomes 7, 11, and 17 in Interphase Human T-Lymphocytes

Lesko, Stephen A.; Callahan, D.E.; Lavilla, M. E.; Wang, Zhengpin; Ts’o, Paul O. P.

doi:10.1006/excr.1995.1258

Cited by 15 publications

(6 citation statements)

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“…Such connections could also provide a mechanism for ordered arrangements of CTs during interphase ( Koss, 1998 ; Nagele et al, 1999 ). However, other groups reported highly variable neighborhoods of both mitotic chromosomes and CTs ( Lesko et al, 1995 ; Allison and Nestor, 1999 ; Cremer et al, 2001 ; Habermann et al, 2001 ). Several studies provided evidence for a nonrandom radial position of specific CTs in the nuclear interior or periphery ( Skalnikova et al, 2000 ; Sun et al, 2000 ; Boyle et al, 2001 ; Cremer et al, 2001 ).…”

Section: Introductionmentioning

confidence: 94%

Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages

Walter¹,

Schermelleh

Cremer

et al. 2003

The Journal of Cell Biology

284

266

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hether chromosomes maintain their nuclear positions during interphase and from one cell cycle to the next has been controversially discussed. To address this question, we performed long-term live-cell studies using a HeLa cell line with GFP-tagged chromatin. Positional changes of the intensity gravity centers of fluorescently labeled chromosome territories (CTs) on the order of several m were observed in early G1, suggesting a role of CT mobility in establishing interphase nuclear architecture. Thereafter, the positions were highly constrained within a range of ‫ف‬ 1 m until the end of G2. To analyze possible changes of chromosome arrangements from one cell cycle to the next, nuclei were photobleached in G2 W maintaining a contiguous zone of unbleached chromatin at one nuclear pole. This zone was stably preserved until the onset of prophase, whereas the contiguity of unbleached chromosome segments was lost to a variable extent, when the metaphase plate was formed. Accordingly, chromatin patterns observed in daughter nuclei differed significantly from the mother cell nucleus. We conclude that CT arrangements were stably maintained from mid G1 to late G2/early prophase, whereas major changes of CT neighborhoods occurred from one cell cycle to the next. The variability of CT neighborhoods during clonal growth was further confirmed by chromosome painting experiments.

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Section: Introductionmentioning

confidence: 94%

Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages

Walter¹,

Schermelleh

Cremer

et al. 2003

The Journal of Cell Biology

284

266

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“…Second, gene-dense chromatin domains and gene-poor domains exhibit different higher-order nuclear patterns ( Croft et al, 1999 ; Boyle et al, 2001 ; Cremer and Cremer, 2001 ; Tanabe et al, 2002 ), although these patterns may well vary from cell type to cell type ( Cremer et al, 2001 ). However, what is not clear is the overall importance of these large-scale correlations compared with the large-scale randomizing processes that also occur ( Lesko et al, 1995 ; Cafourková et al, 2001 ; Edelmann et al, 2001 ; Misteli, 2001 ; Chubb et al, 2002 ). We address this question by looking for quantitative evidence of chromosome–chromosome spatial associations during cell-cycle interphase for all heterologous pairs of normal human chromosomes.…”

Section: Introductionmentioning

confidence: 99%

Chromosomes are predominantly located randomly with respect to each other in interphase human cells

Cornforth

Greulich-Bode

Loucas

et al. 2002

The Journal of Cell Biology

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To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random.

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“…One example of this utilises a distance-based approach, with the rationale that non-uniform positioning of a compartment will be revealed by non-uniform distributions of experimental intercompartment distances compared with those calculated for a uniform simulated distribution [19]. This approach has been successfully applied to chromosome territories by basing homologue separation distances on the distances between the centres of homologous chromosome territories [19,20,33], satellite DNA sequences [28,34], genomic loci in Drosophila cell nuclei [11], and Barr bodies in human fibroblasts [35]. It is also possible to quantify relative spatial proximity in terms of orbital arrangement using the angle formed between compartments and the nuclear centre.…”

Section: Nuclear Positions and Preferencesmentioning

confidence: 99%

Quantitative Analysis of Cell Nucleus Organisation

Shiels

Adams²,

Islam³

et al. 2007

PLoS Comput Biol

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There are almost 1,300 entries for higher eukaryotes in the Nuclear Protein Database. The proteins' subcellular distribution patterns within interphase nuclei can be complex, ranging from diffuse to punctate or microspeckled, yet they all work together in a coordinated and controlled manner within the three-dimensional confines of the nuclear volume. In this review we describe recent advances in the use of quantitative methods to understand nuclear spatial organisation and discuss some of the practical applications resulting from this work.

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The Experimental Homologous and Heterologous Separation Distance Histograms for the Centromeres of Chromosomes 7, 11, and 17 in Interphase Human T-Lymphocytes

Cited by 15 publications

References 0 publications

Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages

Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages

Chromosomes are predominantly located randomly with respect to each other in interphase human cells

Quantitative Analysis of Cell Nucleus Organisation

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