The Experience of Hematopoietic Stem Cell Transplantation From An Emerging Centre in North India.

Abstract

4317 Introduction With a huge burden of hematological and oncological disorders for which hematopoietic stem cell transplant (HSCT) is a curative option, India saw the development of HSCT program from 1986 onwards. Since then, some 10 centres have performed over 1600 transplants until the year 2005, including one-third in thalassemic patients. However, current HSCT facilities are still limited and cannot address the treatment needs of the immense patient load. There is a need for new transplant centres in India. We are describing our experience of HSCT programme at Sir Ganga Ram Hospital, a tertiary hospital in North India, over a period of 3 years. Patients and methods A retrospective study of all patients who underwent HSCT over a period of 3 years, from January 2006 to August 2009 was done and follow-up data was analyzed. Results Thirty-nine transplants (16 allogenic and 23 autologous) were done during this period in 34 patients (26 males and 8 females). The median age of transplant patients was 34 years (11 months-68 years). Children comprised 41.2% of the patients. The indications for 16 allogenic transplants were thalassemia major-6, acute myeloid leukemia (AML)/myelodysplastic syndrome-6, severe aplastic anemia-3 and high-risk acute lymphoblastic leukemia-1. Donors were HLA-matched sibling in 13 cases, HLA-matched relative in 1 and unrelated umbilical cord blood in 2. The source of HSCT was peripheral blood in 8 patients, bone marrow in 6 and umbilical cord blood in 2. Fourteen patients underwent myeloablative transplants and two were given reduced intensity conditioning. Three Donor Lymphocyte infusions were given to two patients. Seven patients (44.8%) are alive and disease free at a median follow-up of 453 days (65-591 days). Thirteen patients showed neutrophil engraftment at a median duration of 14 days (range 11 to 44). Engraftment syndrome was seen after unrelated cord blood transplant in one child. Acute Graft versus host disease (GVHD) was seen in 6 patients, grade I-II was seen in 5 patients, which responded to steroids. Steroid-refractory grade IV GVHD was seen in one child; it did not respond to mycophenolate and infliximab. Massive bleeding (gastro-intestinal-2 and pulmonary-1) occurred in three children. Three patients developed sinusoidal obstruction syndrome. All were given Defibrotide and two responded. A total of 7 allogenic HSCT recipients have died, out of which 2 AML patients died of relapse at 56 and 90 days post-transplant. The main causes of death in 5 other patients were sepsis (3 bacterial and 2 fungal), sinusoidal obstruction syndrome (1 case), acute GVHD (1 case) and chronic GVHD (1 case). Two thalassemic children rejected graft but are alive and transfusion dependent. The main indications for 19 autologous HSCT were multiple myeloma-9, Non-Hodgkin's lymphoma-4, metastatic neuroblastoma-2, relapsed Hodgkin's lymphoma-1, relapsed rhabdomyosarcoma-1, relapsed primitive neuroectodermal tumor (PNET) of the kidney-1 and rejection post cord blood transplant in thalassemia-1. The source of HSCT was peripheral blood in 16 patients and bone marrow in 3. Ten patients (55.6%) are alive and disease-free at a median follow up of 114 days (range 21 - 617 days). Seventeen patients engrafted neutrophils at a median duration of 12 days (range 9 to 30). A child with metastatic neuroblastoma relapsed 8 months post transplant and is on palliative radiotherapy. An adult with indolent lymphoma relapsed 1-year post transplant and is in partial remission two years after relapse. Six autologous HSCT recipients have died. One adult with NHL died of progressive disease one-month post transplant and one with multiple myeloma died of relapse 3-years post transplant. One child with PNET received 4 autologous staged transplants and local radiotherapy with very good partial response but died of candida sepsis 92 days after his last transplant. Three other patients died of bacterial sepsis. At a median follow up of 200 days (range 21 to 1200 days), the estimated overall survival and event free survival for all the transplant population are 67.3% ± 8.6% and 63.5± 8.9% respectively. Overall transplant related mortality is 23.5%, with a decrease from 28.6% to 22.2% after a dedicated HSCT unit with HEPA filtered rooms became functional by mid 2007. Conclusion With our encouraging results we can conclude that it is possible to setup a HSCT unit in developing world and results get better with each passing year. Disclosures: No relevant conflicts of interest to declare.

Hematopoietic stem cell transplantation Experience : A Retrospective analysis from largest center of India’s largest state

“…In western countries, long-term survival was reported as 50-70% in chronic leukemias and 80-90% with aplastic anemias [8]. The overall mortality rate reported in the present study is in accordance with the previous studies wherein mortality rate ranges from 13.7-40.0% [8][9][10][11].…”

“…Previous studies have reported concordant observation suggesting infection and GvHD post-HSCT as major risk factors of mortality [8,10,12]. In a retrospective study from North India reported different reasons of death including sepsis, GvHD, and sinusoidal obstruction syndrome [11]. A study by Nair et al reported mortality rate of 37.7% in patients who underwent allogenic transplantation and GvHD, infections and relapse were the common reasons for death of patients [13].…”

“…Other studies have provided concordant observations in terms of overall survival rates ranging from 72-87% [9,10]. A study by Yadav et al reported overall survival rate of 67.3% [11]. In western countries, long-term survival was reported as 50-70% in chronic leukemias and 80-90% with aplastic anemias [8].…”

Autologous hematopoietic stem cell transplant at a tertiary care centre in India: achieving comparable outcomes with adaptations

“…In another study from India, which also included adult patients with multiple myeloma, Yadav et al demonstrated a 55.6% OS at a median follow-up of 114 days (range: 21-617 days). The median duration of neutrophil engraftment in their study was 12 days (range, 9-30) 17 . Relapsed/refractory HL had a 5-year OS of about 68% 18 and in HR NB, an EFS of 50% has been demonstrated by Landenstein et al in patients undergoing ASCT with BuMel conditioning 19 .…”