The expansion of core group interaction that drives protein folding: A hypothesis based on the cytochrome c fragment complex

Taniuchi, Hiroshi; Fisher, Alice

doi:10.1007/978-94-010-9066-7_58

Peptides 1993

DOI: 10.1007/978-94-010-9066-7_58

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The expansion of core group interaction that drives protein folding: A hypothesis based on the cytochrome c fragment complex

Hiroshi Taniuchi¹,

Alice Fisher²

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2001

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A Study of the Influence of the Hydrophobic Core Residues of Yeast Iso-2-cytochrome c on Phosphate Binding: A Probe of the Hydrophobic Core-Surface Charge Interactions

et al. 2001

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To gain insight into the role of hydrophobic core-surface charge interactions in stabilizing cytochrome c, we investigated the influence of hydrophobic core residues on phosphate binding by mutating residues in yeast iso-2-cytochrome c to those corresponding to iso-l-cytochrome c in various combinations. Heat transition of ultraviolet CD was followed as a function of pH in the presence and absence of phosphate. Thermodynamic parameters were deduced. It was found that the I20V/V43A/M98L mutation in the hydrophobic core, whose locations are remote from the putative phosphate sites, modulates phosphate interactions. The modulation is pH dependent. The I20V/ M98L and V43A mutation effects are nonadditive. The results lead to a model analogous to that of Tsao, Evans, and Wennerstrom, where a domain associated with the ordered hydrophobic core is sensitive to the fields generated by the surface charges. Such an explanation would be in accord with the observed difference in thermal stability between iso-2 and horse cytochromes c.

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A Study of the Influence of the Hydrophobic Core Residues of Yeast Iso-2-cytochrome c on Phosphate Binding: A Probe of the Hydrophobic Core-Surface Charge Interactions

et al. 2001

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show abstract

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The expansion of core group interaction that drives protein folding: A hypothesis based on the cytochrome c fragment complex

Cited by 1 publication

References 16 publications

A Study of the Influence of the Hydrophobic Core Residues of Yeast Iso-2-cytochrome c on Phosphate Binding: A Probe of the Hydrophobic Core-Surface Charge Interactions

A Study of the Influence of the Hydrophobic Core Residues of Yeast Iso-2-cytochrome c on Phosphate Binding: A Probe of the Hydrophobic Core-Surface Charge Interactions

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