The expanding role of CDR1‐AS in the regulation and development of cancer and human diseases

Azari, Hanieh; Mousavi, Pegah; Karimi, Elham; Fatemeh, Sadri; Mahboobeh, Zarei; Rafat, Milad; Shekari, Mohammad

doi:10.1002/jcp.29950

Cited by 13 publications

(9 citation statements)

References 118 publications

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“…We thus found that particular miRNAs, such as miR-671-5p, ciRS-7-5p, and miR-122-5p, are consistently deregulated throughout AD progression. A previous study that found molecular mechanisms of ciRS-7 depletion in AD suggested that ciRS-7 could be an AD treatment target ( 20 ). Similarly, we present results for lesser-known miRNA circulating in AD tissue samples.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA super-resolution imaging in blood for Alzheimer’s disease

Lee¹,

Woo²,

Moon³

et al. 2023

BMB Rep

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We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer’s disease (AD). Here, we report a single-molecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases.

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Section: Resultsmentioning

confidence: 99%

MicroRNA super-resolution imaging in blood for Alzheimer’s disease

Lee¹,

Woo²,

Moon³

et al. 2023

BMB Rep

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“…CDR1as is a closed circular RNA formed from the antisense transcript of the cerebellum degeneration-related antigen 1 (CDR1) gene, whose length is 1,485 bp (Hansen et al, 2013b). CDR1as is highly expressed in the human brain and plays a vital role in midbrain development (Memczak et al, 2013), which might account for its relationship with neurodegenerative diseases, such as Alzheimer and Parkinson's disease (Shi et al, 2017;Azari et al, 2020). And the overexpression of CDR1as in zebrafish resulted in defects in the midbrain region similar to the knockdown of miR-7 (Memczak et al, 2013).…”

Section: The Biological Functions Of Cdr1asmentioning

confidence: 99%

The Emerging Picture of the Roles of CircRNA-CDR1as in Cancer

Jiang

Zeng

Shan

et al. 2020

Front. Cell Dev. Biol.

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Circular RNAs (circRNAs) are covalently closed circular structures without 5′ caps and 3′ tails, which are mainly formed from precursor mRNAs (pre-mRNAs) via back-splicing of exons. With the development of RNA sequencing and bioinformatic analysis, circRNAs were recently rediscovered and found to be widely expressed in the tree of life. Cerebellar degeneration-related protein 1 antisense RNA (CDR1as) is recognized as one of the most well-identified circRNAs. It contains over 70 miR-7 binding sites and can regulate gene activity by sponging miR-7. Increasing numbers of studies have recently demonstrated that CDR1as is abnormally expressed in many types of tumors, such as colorectal cancer, cholangiocarcinoma and osteosarcoma, and plays a vital role in the development of cancer. However, there are few reviews focusing on CDR1as and cancer. Hence, it is important to review and discuss the role of CDR1as in cancer. Here, we first review the main biological features of CDR1as. We then focus on the expression and roles of CDR1as in cancer. Finally, we summarize what is known on the role of CDR1as in cancer and discuss future prospects in this area of research.

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“… 6 Disease progression in human life is largely affected by circRNAs. 7 , 8 CircRNA HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (circ-HACE1, hsa_circ_0077520) was shown to be upregulated in cigarette smoke extract (CSE)-induced COPD cell model, 9 but its function and underlying mechanism in COPD are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Circ-HACE1 Aggravates Cigarette Smoke Extract-Induced Injury in Human Bronchial Epithelial Cells via Regulating Toll-Like Receptor 4 by Sponging miR-485-3p

Zhou

Cao²,

Chai³

et al. 2021

COPD

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Background Smoking is the most common cause of chronic obstructive pulmonary disease (COPD), and the early diagnosis for COPD remains poor. Exploring the molecular mechanism and finding feasible biomarkers will be beneficial for clinical management of COPD. Circular RNAs (circRNAs) are noncoding RNAs that act as miRNA sponges to regulate the expression levels of genes, leading to the changes of cellular phenotypes and disease progression. CircRNA HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (circ-HACE1) was abnormally expressed after the induction of cigarette smoke extract (CSE) in cell model. This study was performed to explore its function and mechanism in COPD. Methods Circ-HACE1, microRNA-485-3p (miR-485-3p) and toll-like receptor 4 (TLR4) detection was performed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability and apoptosis/cell cycle were respectively examined using cell counting kit-8 (CCK-8) and flow cytometry. Inflammatory cytokines were determined by enzyme-linked immunosorbent assay (ELISA). Oxidative stress was evaluated through the measurement of malondialdehyde (MDA) and superoxide dismutase (SOD). The target binding analysis was conducted via dual-luciferase reporter assay. Western blot was employed for protein expression detection of TLR4. Results Circ-HACE1 was overexpressed in smokers or smokers with COPD and CSE upregulated circ-HACE1 expression in 16HBE cells. Knockdown of circ-HACE1 attenuated CSE-stimulated cell viability and cell cycle repression, as well as the enhancement of cell apoptosis, inflammatory response and oxidative stress. MiR-485-3p was a target of circ-HACE1. Circ-HACE1 regulated CSE-induced cell injury via targeting miR-485-3p. TLR4 was a downstream target of miR-485-3p, and miR-485-3p inhibited the CSE-induced cell damages by directly downregulating the level of TLR4. Circ-HACE1/miR-485-3p regulated TLR4 expression in CSE-treated 16HBE cells, and TLR4 overexpression also reversed all effects of si-circ-HACE1 on CSE-treated 16HBE cells. Conclusion These findings elucidated that circ-HACE1 contributed to the CSE-induced cell damages in COPD cell models via regulating TLR4 by acting as the sponge of miR-485-3p.

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The expanding role of CDR1‐AS in the regulation and development of cancer and human diseases

Cited by 13 publications

References 118 publications

MicroRNA super-resolution imaging in blood for Alzheimer’s disease

MicroRNA super-resolution imaging in blood for Alzheimer’s disease

The Emerging Picture of the Roles of CircRNA-CDR1as in Cancer

Circ-HACE1 Aggravates Cigarette Smoke Extract-Induced Injury in Human Bronchial Epithelial Cells via Regulating Toll-Like Receptor 4 by Sponging miR-485-3p

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