The Exosome Total Isolation Chip

Liu, Fei; Vermesh, Ophir; Mani, Vigneshwaran; Ge, Tao; Madsen, Steven J.; Sabour, Andrew F.; Hsu, En‐Chi; Gowrishankar, Gayatri; Kanada, Masamitsu; Jokerst, Jesse V.; Sierra, Raymond G.; Chang, Edwin; Lau, Kenneth; Sridhar, Kaushik; Bermudez, Abel; Pitteri, Sharon J.; Stoyanova, Tanya; Sinclair, Robert; Nair, Viswam S.; Demirci, Utkan

doi:10.1021/acsnano.7b04878

Cited by 274 publications

(238 citation statements)

References 54 publications

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“…Compared with ultracentrifugation, this technique achieved moderately lower separation throughput and exosomes yield. To sort different‐sized EVs from the sample, a multiple filtrations microfluidic chip, called ExoTIC, was developed to separate a subpopulation of EVs (Figure B) . ExoTIC was designed as a modular unit that consisted of membranes with different pore sizes (e.g., 200, 100, 50, and 30 nm).…”

Section: Separation Techniques For Exosomesmentioning

confidence: 99%

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Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Lin

Chen

et al. 2019

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Exosomes are secreted by most cell types and circulate in body fluids. Recent studies have revealed that exosomes play a significant role in intercellular communication and are closely associated with the pathogenesis of disease. Therefore, exosomes are considered promising biomarkers for disease diagnosis. However, exosomes are always mixed with other components of body fluids. Consequently, separation methods for exosomes that allow high‐purity and high‐throughput separation with a high recovery rate and detection techniques for exosomes that are rapid, highly sensitive, highly specific, and have a low detection limit are indispensable for diagnostic applications. For decades, many exosome separation and detection techniques have been developed to achieve the aforementioned goals. However, in most cases, these two techniques are performed separately, which increases operation complexity, time consumption, and cost. The emergence of microfluidics offers a promising way to integrate exosome separation and detection functions into a single chip. Herein, an overview of conventional and microfluidics‐based techniques for exosome separation and detection is presented. Moreover, the advantages and drawbacks of these techniques are compared.

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Section: Separation Techniques For Exosomesmentioning

confidence: 99%

“…The modular units with pore sizes of 200, 100, 50, and 30 nm are connected in series to separate exosomes in several specific size ranges. Reproduced with permission . Copyright 2017, American Chemical Society.…”

Section: Separation Techniques For Exosomesmentioning

confidence: 99%

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Lin

Chen

et al. 2019

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“…This method co-isolates small EVs and some large EVs with high throughput, high yield and low levels of protein contaminants [90]. Liu et al demonstrated a size-based modularised Mf-F system called ExoTIC, which is a modular platform with the ability to isolate EV subpopulations based on size [91] (Figure 2(g)). …”

Section: Microfluidics-based Ev Isolationmentioning

confidence: 99%

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Guo

Tao

Dawn

2018

J of Extracellular Vesicle

131

100

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Extracellular vesicles (EVs), which can be found in almost all body fluids, consist of a lipid bilayer enclosing proteins and nucleic acids from their cells of origin. EVs can transport their cargo to target cells and have therefore emerged as key players in intercellular communication. Their potential as either diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) has generated considerable interest in recent years. However, conventional methods used to study EVs still have significant limitations including the time-consuming and low throughput techniques required, while at the same time the demand for better research tools is getting stronger and stronger. In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advantages, including low cost, low sample volumes, high throughput and precision. This review summarizes recent advances in microfluidics-based technologies, compares conventional and microfluidics-based technologies, and includes a brief survey of recent progress towards integrated “on-a-chip” systems. In addition, this review also discusses the potential clinical applications of “on-a-chip” systems, including both “liquid biopsies” for personalized medicine and DDS devices for precision medicine, and then anticipates the possible future participation of cloud-based portable disease diagnosis and monitoring systems, possibly with the participation of artificial intelligence (AI).

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“…Liu et al reported that three miRNAs, miR-23b-3p, miR-10b-5p, and miR-21-5p, appeared to be promising prognostic biomarkers in a patient with NSCLC. They isolated exosomes from blood from 196 patients and used qPCR assay to determine the enrichment of miRNAs (Liu, Vermesh, et al, 2017; Liu, Yu, et al, 2017). …”

Section: Theranostics Applications Of Exosomes In Lung Cancermentioning

confidence: 99%

“…Moreover, the researchers demonstrated that ExoTIC can sort a heterogeneous population of cancer cell exosomes based on size. They also tested ExoTIC to isolate exosomes from clinical samples, including plasma, urine, and lavage, demonstrating the device’s broad applicability to cancers and other diseases (Liu, Vermesh, et al, 2017; Liu, Yu, et al, 2017). …”

Section: Techniques For Isolation Of Exosomesmentioning

confidence: 99%

Exosomes as Theranostics for Lung Cancer

Srivastava

Amreddy

Razaq

et al. 2018

Advances in Cancer Research

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Extensive research in genetics and genomics has revealed that lung cancer is a physiologically complex and genetically heterogeneous disease. Although molecular targets that can yield favorable response have been identified, those targets cannot be exploited due to the lack of suitable drug carriers. Furthermore, lung cancer often is diagnosed at an advanced stage when the disease has metastasized. Conventional treatments are not effective for treating metastatic lung cancer. Targeted therapeutics while beneficial has challenges that include poor tumor-targeting, off-target effects, and development of resistance to therapy. Therefore, improved drug delivery systems that can deliver drugs specifically to tumor will produce improved treatment outcomes. Exosomes have a natural ability to carry functional biomolecules, such as small RNAs, DNAs, and proteins, in their lumen. This property makes exosomes attractive for use in drug delivery and molecular diagnosis. Moreover, exosomes can be attached to nanoparticles and used for high precision imaging. Exosomes are now considered an important component in liquid biopsy assessments, which are useful for detecting cancers, including lung cancer. Several studies are currently underway to develop methods of exploiting exosomes for use as efficient drug delivery vehicles and to develop novel diagnostic modalities. This chapter summarizes the current status of exosome studies with regard to their use as theranostics in lung cancer. Examples from other cancers have also been cited to illustrate the extensive applicability of exosomes to therapy and diagnosis.

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The Exosome Total Isolation Chip

Cited by 274 publications

References 54 publications

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Exosomes as Theranostics for Lung Cancer

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