The Exosome ‐ A Naturally Secreted Nanoparticle and its Application to Wound Healing

Rani, Sweta; Ritter, Thomas

doi:10.1002/adma.201504009

Cited by 230 publications

(170 citation statements)

References 97 publications

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“…First, exosomes play an important role in cell-cell communication by transferring contained types of bioactive molecules, including proteins, lipids and nucleic acids [60]. Second, nanosized exosomes can easily penetrate through various biobarriers, including the blood-brain barrier [61]; thus, exosomes enable the treatment of previously inaccessible disease foci.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Zhu

Niu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Our previous studies have shown that human urine-derived stem cells (USCs) have great potential as a cell source for cytotherapy and tissue engineering and that extracellular vesicles (EVs) secreted by USCs (USCs-EVs) can prevent diabetes-induced kidney injury in an animal model. The present study was designed to evaluate the effects of USCs-EVs on ischemia repair. Methods: USCs-EVs were isolated and purified by a battery of centrifugation and filtration steps. The USCs-EVs were then characterized by transmission electron microscopy, western blot and tunable resistive pulse sensing techniques. After intramuscularly transplanting USCs-EVs into an ischemic mouse hind-limb, we observed the therapeutic effects of USCs-EVs on perfusion by laser doppler perfusion imaging, angiogenesis and muscle regeneration by histology and immunohistochemistry techniques over 21 days. We subsequently tested whether USCs-EVs can induce the proliferation of a human microvascular endothelial cell line HMEC-1 and a mouse myoblast cell line C2C12 by cell counting kit 8 assay in vitro. Meanwhile, the potential growth factors in the USCs-EVs and supernatants of the USCs cultures were detected by enzyme-linked immunosorbent assay. Results: The USCs-EVs were spherical vesicles with a diameter of 30–150 nm and expressed exosomal markers, such as CD9, CD63 and Tsg101. Ischemic limb perfusion and function were markedly increased in the hind-limb ischemia (HLI) model after USCs-EVs administration. Moreover, angiogenesis and muscle regeneration levels were significantly higher in the USCs-EVs treatment group than in the PBS group. The in vitro experiments showed that USCs-EVs facilitated HMEC-1 and C2C12 cell proliferation in a dose-dependent manner. Conclusions: These results revealed for the first time that USCs-EVs efficiently attenuate severe hind-limb ischemic injury and represent a novel therapy for HLI.

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Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Zhu

Niu

et al. 2018

Cell Physiol Biochem

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“…These SLNs have been tested for delivering bioactive molecules such as opioids like morphine (Kuchler et al, 2010b), resveratrol (Gokce et al, 2012), and silver sulfadiazine (Sandri et al, 2013) for wound healing (Kuchler et al, 2009, 2010a). Exosomes are another form of lipid NPs produced by cells and have been shown to be effective for wound healing (Rani and Ritter, 2015). …”

Section: Nanoparticle-based Wound Therapiesmentioning

confidence: 99%

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

Baker

2016

Front. Bioeng. Biotechnol.

233

196

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Wound healing is an intricate process that requires complex coordination between many cell types and an appropriate extracellular microenvironment. Chronic wounds often suffer from high protease activity, persistent infection, excess inflammation, and hypoxia. While there has been intense investigation to find new methods to improve cutaneous wound care, the management of chronic wounds, burns, and skin wound infection remain challenging clinical problems. Ideally, advanced wound dressings can provide enhanced healing and bridge the gaps in the healing processes that prevent chronic wounds from healing. These technologies have great potential for improving outcomes in patients with poorly healing wounds but face significant barriers in addressing the heterogeneity and clinical complexity of chronic or severe wounds. Active wound dressings aim to enhance the natural healing process and work to counter many aspects that plague poorly healing wounds, including excessive inflammation, ischemia, scarring, and wound infection. This review paper discusses recent advances in the development of biomaterials and nanoparticle therapeutics to enhance wound healing. In particular, this review focuses on the novel cutaneous wound treatments that have undergone significant preclinical development or are currently used in clinical practice.

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“…Preliminary studies have shown that EVs may be involved in wound healing through the control of a number of cellular processes [17,18,19,20,21]. Therefore, this review will summarise the role of EVs derived from predominant cells involved in the wound healing process.…”

Section: Introductionmentioning

confidence: 99%

Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing

Than

Guanzon

Leavesley

et al. 2017

IJMS

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Extracellular vesicles (EVs) are membrane-enclosed vesicles that are released into the extracellular environment by various cell types, which can be classified as apoptotic bodies, microvesicles and exosomes. EVs have been shown to carry DNA, small RNAs, proteins and membrane lipids which are derived from the parental cells. Recently, several studies have demonstrated that EVs can regulate many biological processes, such as cancer progression, the immune response, cell proliferation, cell migration and blood vessel tube formation. This regulation is achieved through the release and transport of EVs and the transfer of their parental cell-derived molecular cargo to recipient cells. This thereby influences various physiological and sometimes pathological functions within the target cells. While intensive investigation of EVs has focused on pathological processes, the involvement of EVs in normal wound healing is less clear; however, recent preliminarily investigations have produced some initial insights. This review will provide an overview of EVs and discuss the current literature regarding the role of EVs in wound healing, especially, their influence on coagulation, cell proliferation, migration, angiogenesis, collagen production and extracellular matrix remodelling.

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The Exosome ‐ A Naturally Secreted Nanoparticle and its Application to Wound Healing

Cited by 230 publications

References 97 publications

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing

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