The Exocyst Complex Regulates Free Fatty Acid Uptake by Adipocytes

Inoue, Mayumi; Akama, Takeshi; Yi-bin, Jiang; Chun, Tae Hwa

doi:10.1371/journal.pone.0120289

Cited by 11 publications

(12 citation statements)

References 49 publications

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“…Several genes that are cis -regulated in the muscle participate in lipid or carbohydrate metabolism, e.g. HIV-1 Tat Interactive Protein 2 ( HTATIP2 ) [14], exocyst complex component 7 ( EXOC7 ) [15] acyl-CoA dehydrogenase short chain ( ACADS ) [16], insulin degrading enzyme ( IDE ) [17], solute carrier family 38 member 9 ( SLC38A9 ) [18], and family with sequence similarity 3 member C ( FAM3C ) [19]. With regard to the liver, hydroxysteroid dehydrogenase like 2 ( HSDL2 ) [20] and lipase A ( LIPA ) [21] genes have also important roles in lipid metabolism.…”

Section: Resultsmentioning

confidence: 99%

About the existence of common determinants of gene expression in the porcine liver and skeletal muscle

et al. 2019

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Background The comparison of expression QTL (eQTL) maps obtained in different tissues is an essential step to understand how gene expression is genetically regulated in a context-dependent manner. In the current work, we have compared the transcriptomic and eQTL profiles of two porcine tissues (skeletal muscle and liver) which typically show highly divergent expression profiles, in 103 Duroc pigs genotyped with the Porcine SNP60 BeadChip (Illumina) and with available microarray-based measurements of hepatic and muscle mRNA levels. Since structural variation could have effects on gene expression, we have also investigated the co-localization of cis- eQTLs with copy number variant regions (CNVR) segregating in this Duroc population. Results The analysis of differential expresssion revealed the existence of 1204 and 1490 probes that were overexpressed and underexpressed in the gluteus medius muscle when compared to liver, respectively (|fold-change| > 1.5, q-value < 0.05). By performing genome scans in 103 Duroc pigs with available expression and genotypic data, we identified 76 and 28 genome-wide significant cis- eQTLs regulating gene expression in the gluteus medius muscle and liver, respectively. Twelve of these cis -eQTLs were shared by both tissues (i.e. 42.8% of the cis- eQTLs identified in the liver were replicated in the gluteus medius muscle). These results are consistent with previous studies performed in humans, where 50% of eQTLs were shared across tissues. Moreover, we have identified 41 CNVRs in a set of 350 pigs from the same Duroc population, which had been genotyped with the Porcine SNP60 BeadChip by using the PennCNV and GADA softwares, but only a small proportion of these CNVRs co-localized with the cis -eQTL signals. Conclusion Despite the fact that there are considerable differences in the gene expression patterns of the porcine liver and skeletal muscle, we have identified a substantial proportion of common cis- eQTLs regulating gene expression in both tissues. Several of these cis -eQTLs influence the mRNA levels of genes with important roles in meat ( CTSF ) and carcass quality ( TAPT1 ), lipid metabolism ( TMEM97 ) and obesity ( MARC2 ), thus evidencing the practical importance of dissecting the genetic mechanisms involved in their expression. Electronic supplementary material The online version of this article (10.1186/s12864-019-5889-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

About the existence of common determinants of gene expression in the porcine liver and skeletal muscle

et al. 2019

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“…Gene silencing of Sec8 also significantly suppressed basal and insulin-dependent free fatty acid uptake by adipocytes (Inoue et al, 2015).…”

Section: Free Fatty Acid Uptakementioning

confidence: 90%

“…In addition, Sec8 partially co‐localized with lipid droplets in 3T3‐L1 cells. Gene silencing of Sec8 also significantly suppressed basal and insulin‐dependent free fatty acid uptake by adipocytes (Inoue et al, ).…”

Section: Sec8 (Exoc4)mentioning

confidence: 99%

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Diverse Functions and Signal Transduction of the Exocyst Complex in Tumor Cells

Tanaka

Goto

Iino

2016

Journal Cellular Physiology

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The exocyst complex is a large conserved hetero-oligomeric complex that consists of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84 subunits. It has been implicated in the targeting of vesicles for regulated exocytosis in various cell types, and is also important for targeted exocytosis of post-Golgi transport vesicles to the plasma membrane. The exocyst complex is essential for membrane growth, secretion, and function during exocytosis and endocytosis. Moreover, the individual components of the complex are thought to act on specific biological processes, such as cytokinesis, ciliogenesis, apoptosis, autophagy, and epithelial-mesenchymal transition (EMT). As a result, recent studies suggest that the exocyst complex may be involved in several diseases such as kidney disease, neuropathogenesis, diabetes, and cancer. In this review, we focus on the diverse functions and cellular signaling pathways of the exocyst complex in various tumors. J. Cell. Physiol. 232: 939-957, 2017. © 2016 Wiley Periodicals, Inc.

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“…5b). While CHCHD3 maintains the structural integrity of mitochondrial cristae 28 , EXOC4 is a component of the exocyst complex involved in the secretion of insulin 29 , as well as lipid and glucose uptake in response to insulin signaling 30 . SNP 3, the most significant variant within the suggestive subset of loci, was closest to motilin ( MLN; Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic variation drives seasonal onset of hibernation in the 13-lined ground squirrel

et al. 2019

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Hibernation in sciurid rodents is a dynamic phenotype timed by a circannual clock. When housed in an animal facility, 13-lined ground squirrels exhibit variation in seasonal onset of hibernation, which is not explained by environmental or biological factors. We hypothesized that genetic factors instead drive variation in timing. After increasing genome contiguity, here, we employ a genotype-by-sequencing approach to characterize genetic variation in 153 ground squirrels. Combined with datalogger records (n = 72), we estimate high heritability (61–100%) for hibernation onset. Applying a genome-wide scan with 46,996 variants, we identify 2 loci significantly (p < 7.14 × 10−6), and 12 loci suggestively (p < 2.13 × 10−4), associated with onset. At the most significant locus, whole-genome resequencing reveals a putative causal variant in the promoter of FAM204A. Expression quantitative trait loci (eQTL) analyses further reveal gene associations for 8/14 loci. Our results highlight the power of applying genetic mapping to hibernation and present new insight into genetics driving its onset.

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The Exocyst Complex Regulates Free Fatty Acid Uptake by Adipocytes

Cited by 11 publications

References 49 publications

About the existence of common determinants of gene expression in the porcine liver and skeletal muscle

About the existence of common determinants of gene expression in the porcine liver and skeletal muscle

Diverse Functions and Signal Transduction of the Exocyst Complex in Tumor Cells

Genetic variation drives seasonal onset of hibernation in the 13-lined ground squirrel

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