The excretion of <i>allo</i>tetrahydrocortisol in human urine

Bush, I. E.; Willoughby, M. L. N.

doi:10.1042/bj0670689

Cited by 135 publications

(59 citation statements)

References 30 publications

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“…Chromatographic analysis of the cortisol metabolites revealed control values similar to those reported by others (17,18). The control values of THS, however, are somewhat higher than have been noted previously (19).…”

Section: Resultssupporting

confidence: 47%

Further Evidence of a Relative Lack of C-21 Hydroxylation in Congenital Adrenal Hyperplasia *

Frantz¹,

Holub²,

Jailer³

1960

J. Clin. Invest.

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Section: Resultssupporting

confidence: 47%

Further Evidence of a Relative Lack of C-21 Hydroxylation in Congenital Adrenal Hyperplasia *

Frantz¹,

Holub²,

Jailer³

1960

J. Clin. Invest.

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“…An aliquot of this eluate was counted for radioactivity. The remainder was dried in vacuo, dissolved in chloroform and chromatographed in the 96 per cent methanol: heptane (1: 1) system of Bush and Willoughby (21). The "free" compound(s) was located and counted as described.…”

Section: Separation Of Plasma Steroidsmentioning

confidence: 99%

The in Vivo Glucuronide Conjugation of Radioactive Etiocholanolone and Androsterone by the Dog Kidney *

Cohn¹,

Hume²

1960

J. Clin. Invest.

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It is recognized that the liver readily conjugates reduced steroids (1) so rapidly that, under normal conditions, reduced steroids are not recovered in an unconjugated form in plasma but rather occur as sulfate (2-4), glucuronide (5), and probably phosphate (6) t Markle Scholar in Medical Science. 1 Compounds referred to are etiocholanolone (3a-hydroxy-etiocholane-1 7-one); androsterone (3a-hydroxyandrostane-17-one); tetrahydrocortisol (3a, 11t8, 17a, 21-tetrahydroxy-pregnane-20-one); tetrahydrocortisone (3a, 17a, 21-trihydroxy-pregnane-11, 20-dione); allo-tetrahydrocortisol (3a, 11,8, 17a, 21-tetrahydroxy-allopregnane-20-one); allo-tetrahydrocortisone (3a, 17a, 21-trihydroxyalldpregnane-11, 20-dione).2 Dubrulle and Claeys (9) measured unconjugated plasma 17-ketosteroids in 16 normal adolescents and found 7 to 28 Atg per 100 ml. 3 Recently, this clinical entity was named etiocholanolone fever in order to differentiate it from the familial Mediterranean fever on one hand, and the many periodic fevers on the other (11). ent in the urine during the same symptomatic episode. When the patients were afebrile and without symptoms, the 24-hour urinary 17-ketosteroid patterns were normal, and unconjugated etiocholanolone was not detected in the plasma. It was postulated that an attack of this type of fever might be associated vith 1) transient overproduction of etiocholanolone and/or 2) an intermittent inability to conjugate. The presence of conjugated etiocholanolone in urine and its absence in plasmiia during an attack of periodic fever is difficult to reconcile unless another organ in addition to the liver is involved in conjugation.The rapid clearance of etiocholanolone and androsterone, after intravenous injection (12) and oral and intravenous administration of testosterone (13-15), suggests the possibility that these compounds may be conjugated by another organ besides the liver. The reports of Dutton (16)

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“…per 24 hours for THF and 1.5 mg. per 24 hours for THE, giving a ratio for THF: THE of 0.14. Romanoff, Seelye, Rodriquez, and Pincus (27) have recently published quantitative data on the excretion of THF and THE combined with allo-THF (28,29) in one group of normal men and in a group of schizophrenic men. There was no essential difference in the ratio of THF:(THE plus allo-THF) between the two groups of men, and the ratio was reported as approximately 1: 2.…”

mentioning

confidence: 99%

Quantitative Determination of the Urinary Cortisolc Metabolites, “Tetrahydro F,” “Allo-Tetrahydro F” and “Tetrahydro E”: Effects of Adrenocorticotropin and Complex Trauma in the Human1

Gold¹,

Singleton²,

Macfarlane³

et al. 1958

J. Clin. Invest.

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Methods for the quantitative estimation of plasma cortisol concentration have been devised by Nelson and Samuels (1), Silber and Porter (2), and Peterson and Wyngaarden (3) who used the sulfuric acid-induced fluorescence developed by Sweat (4). Together with urinary procedures (5, 6) these techniques have been employed to determine the secretion of cortisol by the adrenal in normal and pathological conditions (7-9) and to evaluate the factors involved in the "removal" of cortisol from the plasma in surgery (10-13) and liver disease (14-16).Although a considerable amount of work has been done in isolating and identifying such urinary metabolites of cortisol as THF8 and THE (17-22) and their interconversions (23-25), much less has been done to relate the quantitative transformations of these urinary metabolites from cortisol under normal and pathological conditions. In 1953, deCourcy, Bush, Gray, and Lunnon (26) reported daily excretion values for two chromatographically separated urinary steroids corresponding in migration rates to THF and THE, in 10 1 This work was suported in part by a grant from the Atomic Energy Commission. It was sponsored and supported in part by the Subcommittee on Metabolism in Trauma, Advisory Committee on Metabolism, Office of the Surgeon General, Department of the Army, through a contract (DA-49-MD472) with Harvard University.The assistance of Winthrop Laboratories, Inc., and The Upjohn Co. is gratefully acknowledged.2 Present address: The Medical College of St. Bartholomew's Hospital, London. 3 Compounds referred to are the following: THF, tetrahydrocortisol (3a,11p,17a,21-tetrahydroxypregnan-20-one ); allo-THF (3a,1 1,l17a,21-tetrahydroxyallopregnan-20-one); THE, tetrahydrocortisone (3a,17a,21-trihydroxypregnan-11,20-dione); F, cortisol (llfi,17a,21-trihydroxypregnan-3,20-dione); E, cortisone (17a,21-dihydroxypregnan-3,11,20-trione).normal men. The mean values were 212 tg. per 24 hours for THF and 1.5 mg. per 24 hours for THE, giving a ratio for THF: THE of 0.14. Romanoff, Seelye, Rodriquez, and Pincus (27) have recently published quantitative data on the excretion of THF and THE combined with allo-THF (28, 29) in one group of normal men and in a group of schizophrenic men. There was no essential difference in the ratio of THF:(THE plus allo-THF) between the two groups of men, and the ratio was reported as approximately 1: 2. Cope and Hurlock (30) published data on the excretion of urinary THF and THE in several normal and surgical cases. Their results indicated increased excretion of these metabolites after surgery but did not show any other definite pattern of alteration of the metabolism of cortisol, although they state that "a tendency can be observed for tetrahydro-compound F excretion to rise more than does tetrahydrocortisone." In a preliminary report, Gold, Macfarlane, and Moore (31) showed that the relative proportions of the two urinary cortisol metabolites, THF and THE, were related to situations of "stress" and adrenocorticotropin (ACTH) administration. It is the purpo...

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The excretion of allotetrahydrocortisol in human urine

Cited by 135 publications

References 30 publications

Further Evidence of a Relative Lack of C-21 Hydroxylation in Congenital Adrenal Hyperplasia *

Further Evidence of a Relative Lack of C-21 Hydroxylation in Congenital Adrenal Hyperplasia *

The in Vivo Glucuronide Conjugation of Radioactive Etiocholanolone and Androsterone by the Dog Kidney *

Quantitative Determination of the Urinary Cortisolc Metabolites, “Tetrahydro F,” “Allo-Tetrahydro F” and “Tetrahydro E”: Effects of Adrenocorticotropin and Complex Trauma in the Human1

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