The exchange of 131I-labelled lipid and 131I-labelled protein between red cells and serum

Studies of the Composition and Structure of Serum Lipoproteins. Isolation and Characterization of Very High Density Lipoproteins of Human Serum*

“…The ApoA and HDL were labeled with iodine-131! according to the procedure of Jones and Gardner (1962). The radioactive proteins were passed through an ionexchange column, Ioresin (Abbott Laboratories, Chicago,111.…”

Desorption of Some Toxic Heavy Metals from Human Erythrocytes in vitro

“…But this desorption is a fairly complete one, and only by suspending erythrocytes in their own plasma can the more firmly bound surface molecules be exchanged against proteins and lipids of the liquid used for washing. These more firmly bound compo nents represent lipids from 80 to 95% and plasma proteins up to 25% of all the electrophoretically separated fractions of serum proteins (8). The protein film covers about 10% of the erythrocytic surface (14).…”

“…According to J ones and G ardner (8), even after 7 washings, 25-80% of the proteins bound to the erythrocytic surface remains deposited in the cells, whereas lipids are removed almost completely. Therefore a complete desoiption of all the lead-carrying molecules may not be expected.…”

“…Samples of them were then washed 6 times more in either phosphate-buffer pH 7.4, citrated 102 Witschi, Desorption of Some Toxic Heavy Metals XXXIV/1965 ments of J ones and G a r d n er (8), we suspended the erythrocytes, which had been incubated with metals and washed repeatedly beforehand, in their own plasma or in individual plasma fractions. With this procedure, a considerable amount of plasma components can be removed from the erythrocytic surface.…”

Der Einfluß dysproteinämischer Seren auf die Resistenz normaler Erythrozyten

“…Durch Anwendung radioaktiv markierter, reiner Plasmaprotcine war es uns [6] und anderen Autoren [8,9] möglich, die Existenz eines Plasmaproteinfilmes an der Erythro zytenoberfläche zu beweisen und darüberhinaus auch die Zusammensetzung des Eiweiss filmes abzuschätzen sowie die Bedingungen zu studieren, mit denen sich die Serumproteine an die Erythrozytenmembran binden. Auf Grund unterschiedlich hoher Bindungsneigung der einzelnen Plasmaproteine zur Erythrozytenoberfläche unterscheidet sich die prozentuale Zusammensetzung der Proteine im Serum grundsätzlich von der Zusammensetzung am Erythrozyten.…”