The evolutionary conserved iron-sulfur protein TCR controls P700 oxidation in photosystem I

Trinh, Mai Duy Luu; Miyazaki, Daichi; Ono, Sumire; Nomata, Jiro; Kono, Mitsuhiko; Mino, Hiroyuki; Niwa, Tatsuya; Okegawa, Yuki; Motohashi, Ken; Taguchi, Hideki; Hisabori, Toru; Masuda, Shinji

doi:10.1016/j.isci.2021.102059

Cited by 3 publications

(3 citation statements)

References 144 publications

(194 reference statements)

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“…This generated more S/N hybrid species when interacting with reactive sulfur species (RSS) and reactive nitrogen species (RNS) [ 3 , 4 ], which put the sulfur-based metabolism at high risk for rapid oxidation. All these consequences led the organisms to have controlled electron transfer during the evolutionary process [ 5 ]. This occurred within the set point of redox potential inside the same cell at different sites [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Redox Status Is the Mainstay of SARS-CoV-2 and Host for Producing Therapeutic Opportunities

Thirupathi

Radák

et al. 2022

Antioxidants

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Over hundreds of years, humans have faced multiple pandemics and have overcome many of them with scientific advancements. However, the recent coronavirus disease (COVID-19) has challenged the physical, mental, and socioeconomic aspects of human life, which has introduced a general sense of uncertainty among everyone. Although several risk profiles, such as the severity of the disease, infection rate, and treatment strategy, have been investigated, new variants from different parts of the world put humans at risk and require multiple strategies simultaneously to control the spread. Understanding the entire system with respect to the commonly involved or essential mechanisms may be an effective strategy for successful treatment, particularly for COVID-19. Any treatment for COVID-19 may alter the redox profile, which can be an effective complementary method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry and further replication. Indeed, redox profiles are one of the main barriers that suddenly shift the immune response in favor of COVID-19. Fortunately, several redox components exhibit antiviral and anti-inflammatory activities. However, access to these components as support elements against COVID-19 is limited. Therefore, understanding redox-derived species and their nodes as a common interactome in the system will facilitate the treatment of COVID-19. This review discusses the redox-based perspectives of the entire system during COVID-19 infection, including how redox-based molecules impact the accessibility of SARS-CoV-2 to the host and further replication. Additionally, to demonstrate its feasibility as a viable approach, we discuss the current challenges in redox-based treatment options for COVID-19.

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Section: Introductionmentioning

confidence: 99%

Redox Status Is the Mainstay of SARS-CoV-2 and Host for Producing Therapeutic Opportunities

Thirupathi

Radák

et al. 2022

Antioxidants

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“…We previously screened Arabidopsis genes that have the following properties: (a) specifically conserved in oxygenic phototrophs, (b) contain the chloroplast transit peptide, and (c) co-express with NPQrelated genes [25][26][27]. Through this screening, we isolated and characterized four genes encoding chloroplast proteins: fluctuating-light acclimation protein 1 (FLAP1) [25], day-length-dependent delayed-greening1 (DLDG1) [26], triplet cysteine repeat protein (TCR) [27], and the DLDG1 homolog Ycf10 encoded in the chloroplast genome [28]. TCR localizes in the chloroplast stroma and accepts electrons from ferredoxin to oxidize PSI [27].…”

mentioning

confidence: 99%

“…Through this screening, we isolated and characterized four genes encoding chloroplast proteins: fluctuating-light acclimation protein 1 (FLAP1) [25], day-length-dependent delayed-greening1 (DLDG1) [26], triplet cysteine repeat protein (TCR) [27], and the DLDG1 homolog Ycf10 encoded in the chloroplast genome [28]. TCR localizes in the chloroplast stroma and accepts electrons from ferredoxin to oxidize PSI [27]. FLAP1 localizes in both the thylakoid and envelope membranes of chloroplasts, and is thought to be involved in the regulation of NPQ under variable light conditions [25].…”

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confidence: 99%

Arabidopsis mutants lacking DLDG1 and non‐photochemical quenching‐related proteins reveal the regulatory role of DLDG1 in chloroplast pH homeostasis

Suzuki

Masuda

2023

FEBS Letters

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The control of pH in chloroplasts is important to regulate photosynthesis, although details of the precise regulatory mechanisms of H + homeostasis in chloroplasts are not fully understood. We recently found that the cyanobacterial PxcA homolog DLDG1 is involved in plastidial pH control. PxcA and DLDG1 have been thought to control light-dependent H + extrusion across the cyanobacterial cytoplasmic and chloroplast envelope membranes, respectively. To investigate DLDG1-dependent pH control in chloroplasts, we crossed the dldg1 mutant with various mutants lacking known nonphotochemical quenching (NPQ)-related proteins, such as fluctuating-light acclimation protein 1 (FLAP1), PsbS/NPQ4, and proton gradient regulation 5 (PGR5). Phenotypes of these double mutants revealed that PsbS works upstream of DLDG1, PGR5 affects NPQ independently from DLDG1, and the DpH regulation by FLAP1 and DLDG1 are independent of each other.

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Restricting electron flow at cytochrome b6f when downstream electron acceptors are severely limited

et al. 2023

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Photosynthetic organisms frequently experience abiotic stress that restricts their growth and development. Under such circumstances, most absorbed solar energy cannot be used for CO2 fixation and can cause the photoproduction of reactive oxygen species (ROS) that can damage the photosynthetic reaction centers of photosystem I and II (PSI and PSII), resulting in a decline in primary productivity. This work describes a biological ‘switch’ in the green alga Chlamydomonas reinhardtii that reversibly restricts photosynthetic electron transport (PET) at the cytochrome b6f (Cyt b6f) complex when the capacity for accepting electrons downstream of PSI is severely limited. We specifically show this restriction in STARCHLESS6 (sta6) mutant cells, which cannot synthesize starch when they are limited for nitrogen (growth inhibition) and subjected to a dark-to-light transition. This restriction represents a form of photosynthetic control that causes diminished electron flow to PSI and thereby prevents PSI photodamage but does not appear to rely on a ΔpH. Furthermore, when electron flow is restricted, the plastid alternative oxidase (PTOX) becomes active, functioning as an electron valve that dissipates some excitation energy absorbed by PSII and allows the formation of a proton motive force (PMF) that would drive some ATP production [potentially sustaining PSII repair and non-photochemical quenching (NPQ)]. The restriction at the Cyt b6f complex can be gradually relieved with continued illumination. This study provides insights into how photosynthetic electron transport responds to a marked reduction in availability of downstream electron acceptors and the protective mechanisms involved.

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The evolutionary conserved iron-sulfur protein TCR controls P700 oxidation in photosystem I

Cited by 3 publications

References 144 publications

Redox Status Is the Mainstay of SARS-CoV-2 and Host for Producing Therapeutic Opportunities

Redox Status Is the Mainstay of SARS-CoV-2 and Host for Producing Therapeutic Opportunities

Arabidopsis mutants lacking DLDG1 and non‐photochemical quenching‐related proteins reveal the regulatory role of DLDG1 in chloroplast pH homeostasis

Restricting electron flow at cytochrome b6f when downstream electron acceptors are severely limited

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