The evolutionarily conserved MOS4-associated complex

Johnson, Kaeli C. M.; Dong, Oliver Xiaoou; Li, Xin

doi:10.2478/s11535-011-0043-7

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…It has been suspected that MAC is also involved in splicing in plants, like its orthologs in human and yeast (Johnson et al, 2011;Koncz et al, 2012). Indeed, our RNA-seq analyses uncovered intron retention defects in three MAC subunit mutants, thus linking MAC with pre-mRNA splicing.…”

Section: Discussionmentioning

confidence: 68%

“…MAC is a highly conserved complex among eukaryotes, with its orthologs known as the NINETEEN COMPLEX (NTC) or Prp19 complex (Prp19C) in yeast and humans. The Arabidopsis thaliana MAC, yeast, and human NTC/Prp19C all associate with the spliceosome and are predicted to share conserved functions in splicing in all three systems (Monaghan et al, 2009;Johnson et al, 2011;Koncz et al, 2012;Deng et al, 2016). Arabidopsis CDC5 is a MYB-related transcription factor and PRL1 is a conserved nuclear WD-40 protein.…”

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis MOS4-Associated Complex Promotes MicroRNA Biogenesis and Precursor Messenger RNA Splicing

et al. 2017

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In , the MOS4-ASSOCIATED COMPLEX (MAC) is required for defense and development. The evolutionarily conserved, putative RNA helicase MAC7 is a component of the Arabidopsis MAC, and the human MAC7 homolog, Aquarius, is implicated in pre-mRNA splicing. Here, we show that, a partial loss-of-function mutant in , and two other MAC subunit mutants, and (), exhibit reduced microRNA (miRNA) levels, indicating that MAC promotes miRNA biogenesis. The mutant shows reduced primary miRNA (pri-miRNA) levels without affecting miRNA gene () promoter activity or the half-life of pri-miRNA transcripts. As a nuclear protein, MAC7 is not concentrated in dicing bodies, but it affects the localization of HYPONASTIC LEAVES1 (HYL1), a key protein in pri-miRNA processing, to dicing bodies. Immunoprecipitation of HYL1 retrieved 11 known MAC subunits, including MAC7, indicating association between HYL1 and MAC. We propose that MAC7 links transcription to pri-miRNA processing. RNA-seq analysis showed that downregulated genes in MAC subunit mutants are mostly involved in plant defense and stimulus responses, confirming a role of MAC in biotic and abiotic stress responses. We also discovered global intron retention defects in mutants in three subunits of MAC, thus linking MAC function to splicing in Arabidopsis.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

The Arabidopsis MOS4-Associated Complex Promotes MicroRNA Biogenesis and Precursor Messenger RNA Splicing

et al. 2017

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“…PRL1 belongs to the NineTeen Complex (NTC) or MOS4-associated complex (MAC), a protein complex with 19 conserved members in yeast, human and plants. MAC is involved in spliceosome assembly and pre-mRNA splicing in all eukaryotic model organisms (Monaghan et al ., 2009; reviewed in Johnson et al ., 2011 and Koncz et al ., 2012). Arabidopsis TOUGH colocalizes or interacts with splicing factor SR proteins, indicating a potential role in general pre-mRNA splicing (Calderon-Villalobos et al ., 2005; Ren et al ., 2012b).…”

Section: Microrna Biogenesis In Plantsmentioning

confidence: 99%

The ‘how’ and ‘where’ of plant microRNAs

2017

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Contents 1002I.1002II.1007III.1010IV.10131013References1013 Summary MicroRNAs (miRNAs) are small non‐coding RNAs, of typically 20–24 nt, that regulate gene expression post‐transcriptionally through sequence complementarity. Since the identification of the first miRNA, lin‐4, in the nematode Caenorhabditis elegans in 1993, thousands of miRNAs have been discovered in animals and plants, and their regulatory roles in numerous biological processes have been uncovered. In plants, research efforts have established the major molecular framework of miRNA biogenesis and modes of action, and are beginning to elucidate the mechanisms of miRNA degradation. Studies have implicated restricted and surprising subcellular locations in which miRNA biogenesis or activity takes place. In this article, we summarize the current knowledge on how plant miRNAs are made and degraded, and how they repress target gene expression. We discuss not only the players involved in these processes, but also the subcellular sites in which these processes are known or implicated to take place. We hope to raise awareness that the cell biology of miRNAs holds the key to a full understanding of these enigmatic molecules.

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“…The Arabidopsis MAC, as the yeast and human NTC/Prp19C, associates with the spliceosome. All three complexes are predicted to share conserved functions in regulating splicing (Monaghan et al, 2009; Johnson et al, 2011). In Arabidopsis, loss-of-function of MAC, as in mac3a mac3b and prl1 prl2 double mutants, impairs miRNA biogenesis (Jia et al, 2017; Li et al, 2018).…”

Section: Mirna Biogenesismentioning

confidence: 99%

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

Fonseca

Rubio

2019

Front. Plant Sci.

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CULLIN4 (CUL4) RING ligase (CRL4) complexes contain a CUL4 scaffold protein, associated to RBX1 and to DDB1 proteins and have traditionally been associated to protein degradation events. Through DDB1, these complexes can associate with numerous DCAF proteins, which directly interact with specific targets promoting their ubiquitination and subsequent degradation by the proteasome. A characteristic feature of the majority of DCAF proteins that associate with DDB1 is the presence of the DWD motif. DWD-containing proteins sum up to 85 in the plant model species Arabidopsis. In the last decade, numerous Arabidopsis DWD proteins have been studied and their molecular functions uncovered. Independently of whether their association with CRL4 has been confirmed or not, DWD proteins are often found as components of additional multimeric protein complexes that play key roles in essential nuclear events. For most of them, the significance of their complex partnership is still unexplored. Here, we summarize recent findings involving both confirmed and putative CRL4-associated DCAF proteins in regulating nuclei architecture remodelling, DNA damage repair, histone post-translational modification, mRNA processing and export, and ribosome biogenesis, that definitely have an impact in gene expression and de novo protein synthesis. We hypothesized that, by maintaining accurate levels of regulatory proteins through targeted degradation and transcriptional control, CRL4 complexes help to surveil nuclear processes essential for plant development and survival.

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The evolutionarily conserved MOS4-associated complex

Cited by 10 publications

References 46 publications

The Arabidopsis MOS4-Associated Complex Promotes MicroRNA Biogenesis and Precursor Messenger RNA Splicing

The Arabidopsis MOS4-Associated Complex Promotes MicroRNA Biogenesis and Precursor Messenger RNA Splicing

The ‘how’ and ‘where’ of plant microRNAs

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

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