The evolution of the plastid phosphate translocator family

Distinctive mitochondrial and chloroplast components contributing to the maintenance of carbon balance during plant growth at elevated CO2

“…The analysis also showed the presence of a second transporter that, similar to other Solanaceae, grouped separately from GPT2. Based on these results and the prior analysis 44 we conclude that N. tabacum, like other asterids, contains both the GPT1 and GPT3 groups of GPT transporters (Figure 2). In the text below, we use the terms GPT2 and GPT3 interchangeably, depending upon the dicot species in question.…”

“…As background, the chloroplast inner envelope membrane contains a family of plastid phosphate translocators that includes the triose phosphate/phosphate translocator (TPP), the phosphoenolpyruvate/phosphate translocator, the glucose-6-phosphate/phosphate translocator (GPT) and the xylulose-5-phosphate/phosphate translocator. [42][43][44] In almost all dicots, the GPT subfamily includes two distinct groups termed GPT1 and GPT2. The Arabidopsis thaliana genome contains one GPT1 and one GPT2 gene.…”

“…46 Recently, an extensive phylogenetic analysis of plastid phosphate translocators reported that, within the asterid clade of dicots, which includes the family Solanaceae, GPT2 is replaced by another GPT group that was designated GPT3. 44 N. tabacum is a member of the Solanaceae but was not included in this prior analysis. Hence, we examined the relationship between N. tabacum GPT protein sequences and those of other dicots, including the well-characterized Arabidopsis GPT1 and GPT2 proteins (Figure 2).…”

Assessment of genetically modified oilseed rape 73496 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐NL‐2012‐109)

“…The GMO Panel considered the adequacy of the selected endpoints for the identification of possible unintended effects related to the reorganisation at the insertion site and the PGtpt gene disruption (see Section 3.1.2). The information available from the published literature suggests that phenotypic changes may be associated with a deficiency of tpt gene activity in dicot plants (such as Arabidopsis thaliana and tobacco) indicating a possible impact on growth, biomass and yield (Häusler et al., 2000; Schneider et al., 2002; Bockwoldt et al., 2019). The agronomic/phenotypic data set included several endpoints that are directly related to those parameters (i.e.…”

“…The GMO Panel assessed the adequacy of the compositional endpoint data set for the identification of potential unintended effects related to the reorganisation at the insertion site and the PGtpt gene disruption (see Section 3.1.2). Data in Arabidopsis and tobacco plants suggest that the potential deficiency of tpt gene would be compensated by both continuous accelerated starch turnover and export of neutral sugars from the stroma (Bockwoldt et al., 2019; Häusler et al., 2000; Schneider et al., 2002). The GMO Panel considers that the compositional endpoints evaluated (key nutrients, key toxicants and anti‐nutrients) are comprehensive enough to capture potential modifications in metabolic pathways of the GM plant, induced by the above changes, that may be of relevance for food and feed assessment, and that no specific hypotheses requiring further compositional investigations are identified.…”