The Evolution of Bladder Augmentation: From Creating a Reservoir to Reconstituting an Organ

Jednak, Roman

doi:10.3389/fped.2014.00010

Cited by 21 publications

(14 citation statements)

References 114 publications

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“…Besides, this sophisticated technology ideally renders possible to produce personalized organs in a perfectly controlled form. Clinical successes in this bioengineering field have only been achieved so far in more functionally simple organs, such as trachea, bronchi [ 67 , 154 ], blood vessels [ 155 , 156 , 157 ], and bladder [ 35 , 158 , 159 , 160 ]. Technical challenges related to the sensitivities of the living cells and tissue construction have to be addressed by integrating the multidisciplinary knowledge of a work team composed of experts in bioengineering, material science, biology, chemistry, physics, and medicine [ 161 , 162 ].…”

Section: Urinary Bladder Bioprintingmentioning

confidence: 99%

Bioengineering Approaches for Bladder Regeneration

Serrano‐Aroca

Donoso

Moreno‐Manzano

2018

IJMS

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Current clinical strategies for bladder reconstruction or substitution are associated to serious problems. Therefore, new alternative approaches are becoming more and more necessary. The purpose of this work is to review the state of the art of the current bioengineering advances and obstacles reported in bladder regeneration. Tissue bladder engineering requires an ideal engineered bladder scaffold composed of a biocompatible material suitable to sustain the mechanical forces necessary for bladder filling and emptying. In addition, an engineered bladder needs to reconstruct a compliant muscular wall and a highly specialized urothelium, well-orchestrated under control of autonomic and sensory innervations. Bioreactors play a very important role allowing cell growth and specialization into a tissue-engineered vascular construct within a physiological environment. Bioprinting technology is rapidly progressing, achieving the generation of custom-made structural supports using an increasing number of different polymers as ink with a high capacity of reproducibility. Although many promising results have been achieved, few of them have been tested with clinical success. This lack of satisfactory applications is a good reason to discourage researchers in this field and explains, somehow, the limited high-impact scientific production in this area during the last decade, emphasizing that still much more progress is required before bioengineered bladders become a commonplace in the clinical setting.

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Section: Urinary Bladder Bioprintingmentioning

confidence: 99%

Bioengineering Approaches for Bladder Regeneration

Serrano‐Aroca

Donoso

Moreno‐Manzano

2018

IJMS

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“…The sheep may be adapted as a valid model for its ability to perform a variety of complex tasks and behaviors involving goal-oriented motor coordination, emotion, facial recognition and memory-based performance 1 – 4 . In addition, the convoluted brain of the sheep may be considered as an alternative to rodents in translational experimental neuroscience 1 , 5 . As cognitive processes or emotional states can often be reflected in brain responses 6 , a better understanding of affective states of sheep in various environments or as a response to different stimuli will benefit both neuroscience and animal welfare.…”

Section: Introductionmentioning

confidence: 99%

Reliability of fNIRS for noninvasive monitoring of brain function and emotion in sheep

Chincarini

Costa

Qiu

et al. 2020

Sci Rep

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The aim of this work was to critically assess if functional near infrared spectroscopy (fNIRS) can be profitably used as a tool for noninvasive recording of brain functions and emotions in sheep. We considered an experimental design including advances in instrumentation (customized wireless multi-distance fNIRS system), more accurate physical modelling (two-layer model for photon diffusion and 3D Monte Carlo simulations), support from neuroanatomical tools (positioning of the fNIRS probe by MRI and DTI data of the very same animals), and rigorous protocols (motor task, startling test) for testing the behavioral response of freely moving sheep. Almost no hemodynamic response was found in the extra-cerebral region in both the motor task and the startling test. In the motor task, as expected we found a canonical hemodynamic response in the cerebral region when sheep were walking. In the startling test, the measured hemodynamic response in the cerebral region was mainly from movement. Overall, these results indicate that with the current setup and probe positioning we are primarily measuring the motor area of the sheep brain, and not probing the too deeply located cortical areas related to processing of emotions.

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“…Bladder augmentation remains one of the greatest surgical challenges in the field of urology. Conventional bladder reconstruction using gastrointestinal tissue is associated with a series of complications (mucus production, bacterial colonization, electrolyte imbalances, or malignancy), significant morbidity, and functional alterations ( 1 ). Tissue engineering technique can circumvent many of these limitations and therefore has become considered as a potential alternative for bladder reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Human Urine-derived Stem Cells Seeded Surface Modified Composite Scaffold Grafts for Bladder Reconstruction in a Rat Model

et al. 2015

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We conducted this study to investigate the synergistic effect of human urine-derived stem cells (USCs) and surface modified composite scaffold for bladder reconstruction in a rat model. The composite scaffold (Polycaprolactone/Pluronic F127/3 wt% bladder submucosa matrix) was fabricated using an immersion precipitation method, and heparin was immobilized on the surface via covalent conjugation. Basic fibroblast growth factor (bFGF) was loaded onto the heparin-immobilized scaffold by a simple dipping method. In maximal bladder capacity and compliance analysis at 8 weeks post operation, the USCsscaffold heparin-bFGF group showed significant functional improvement (2.34 ± 0.25 mL and 55.09 ± 11.81 μL/cm H2O) compared to the other groups (2.60 ± 0.23 mL and 56.14 ± 9.00 μL/cm H2O for the control group, 1.46 ± 0.18 mL and 34.27 ± 4.42 μL/cm H2O for the partial cystectomy group, 1.76 ± 0.22 mL and 35.62 ± 6.69 μL/cm H2O for the scaffold group, and 1.92 ± 0.29 mL and 40.74 ± 7.88 μL/cm H2O for the scaffold heparin-bFGF group, respectively). In histological and immunohistochemical analysis, the USC-scaffold heparin-bFGF group showed pronounced, well-differentiated, and organized smooth muscle bundle formation, a multi-layered and pan-cytokeratin-positive urothelium, and high condensation of submucosal area. The USCs seeded scaffold heparin-bFGF exhibits significantly increased bladder capacity, compliance, regeneration of smooth muscle tissue, multi-layered urothelium, and condensed submucosa layers at the in vivo study.

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The Evolution of Bladder Augmentation: From Creating a Reservoir to Reconstituting an Organ

Cited by 21 publications

References 114 publications

Bioengineering Approaches for Bladder Regeneration

Bioengineering Approaches for Bladder Regeneration

Reliability of fNIRS for noninvasive monitoring of brain function and emotion in sheep

Human Urine-derived Stem Cells Seeded Surface Modified Composite Scaffold Grafts for Bladder Reconstruction in a Rat Model

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