The evolution and functional characterization of CXC chemokines and receptors in lamprey

Sun, Zhaosheng; Qin, Yuting; Liu, Danjie; Wang, Bangjie; Zhao, Jia; Wang, Junya; Gao, Qian; Zou, Jun; Pang, Yue

doi:10.1016/j.dci.2020.103905

Cited by 23 publications

(24 citation statements)

References 35 publications

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“…Total RNA was extracted, and partial gene sequences were obtained as described previously ( 34 ). The full-length cDNA sequences of Lm Panx1, Lm Cx32.2, Lm Cx32.3, Lm Cx32.7, and Lm Cx43 were then cloned and verified by our previous methods ( 35 ). All primers are summarized in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Programs on the NCBI website ( ) and Expasy website ( ) were used to analyze nucleotide and protein sequences. Phylogenetic trees and multiple sequence alignment of Panx1, Cx32, and Cx43 were analyzed using the ClustalW, GeneDoc, and MEGA 5.1 program, according to the method described previously ( 35 ). The Ensembl and NCBI genome databases were analyzed to infer the genomic organization and syntenic relationships.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Pannexin1, Connexin32, and Connexin43 in Spotted Sea Bass (Lateolabrax maculatus): They Are Important Neuro-Related Immune Response Genes Involved in Inflammation-Induced ATP Release

et al. 2022

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Many immunological diseases can be treated by regulating neurobehavior, in which extracellular ATP is a vital member of endogenous danger-associated molecular pattern signaling molecule that plays a crucial part in innate neuro-related immunity. It is actively released through pannexin (Panx) and connexin (Cx) hemichannels from activated or stressed cells during inflammation, injury, or apoptosis. In addition to participating in ATP release, Panxs and Cxs also have crucial immune functions. In this study, pannexin1, three connexin32 isoforms and connexin43 were identified and characterized in spotted sea bass (Lateolabrax maculatus), which were named LmPanx1, LmCx32.2, LmCx32.3, LmCx32.7, and LmCx43. Their similar topological structures were discovered by sequence analysis: a relatively unconserved C-terminal region and four highly conserved transmembrane (TM) domains, and so on. Each extracellular (ECL) region of Panx1 has two conserved cysteine residues. Unlike Panx1, each ECL region of Cx32 and Cx43 contains three conserved cysteine residues, forming two conserved motifs: CX6CX3C motif in ECL1 and CX4CX5C motif in ECL2. Furthermore, Panx1 and Cx43 share similar genomic organization and synteny with their counterparts in selected vertebrates. Cx32 and CX43 were located in the same locus in fish, but diverged into two loci from amphibian. Moreover, despite varying expression levels, the identified genes were constitutively expressed in all examined tissues. All genes were upregulated by PAMP [lipopolysaccharide and poly(I:C)] stimulation or bacterial infection in vivo and in vitro, but they were downregulated in the brain at 6 or 12 h after stimulation. Especially, the three LmCx32 isoforms and LmCx43 were upregulated by ATP stimulation in primary head kidney leukocytes; however, downregulation of LmCx32.3 and LmCx43 expression were noted at 12 h. Conversely, ATP treatment inhibited the expression of LmPanx1. Importantly, we showed that the spotted sea bass Panx1, Cx43, and Cx32 were localized on the cellular membrane and involved in inflammation-induced ATP release. Taken together, our results demonstrated that Panx1, Cx32, and Cx43 are important neuro-related immune response genes involved in inflammation-induced ATP release.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Characterization of Pannexin1, Connexin32, and Connexin43 in Spotted Sea Bass (Lateolabrax maculatus): They Are Important Neuro-Related Immune Response Genes Involved in Inflammation-Induced ATP Release

et al. 2022

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“…Several studies have reported that CXCR6 is absent in teleost fish [ 18 , 19 , 20 ]. However, of these 10 four-eyed sleeper CXCR transcripts, one was predicted as CXCR6 .…”

Section: Resultsmentioning

confidence: 99%

“…Zou et al have classified the fish CXCRs in three distinct groups: Agnatha (jawless fish), Chondrichthyes (cartilaginous fish), and Osteichthyes (teleost fish) [ 18 ]. The jawless fish sea lamprey has four CXCR genes ( CXCR4 , CXCR7a/7b , and one CXCR related to CXCR1 / 2 ) [ 20 , 25 ]. After second round genome duplication, cartilaginous fish, such as elephant shark, have developed more CXCR genes ( CXCR1 / 2 / 4 / 5 / 6 ) to adapt the emergence of RAG-mediated adaptive immune system [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Because chemokines have high affinity to chemokine receptors, several chemokines can bind to one or two chemokine receptors. For example, the interactions of CXCL8-CXCR1, CXCL8-CXCR2, CXCL12-CXCR4, and CXCL12-CXCR7 have been demonstrated to be critical for directing immune response, wound repair and cell migration in mammals [ 9 , 20 , 30 ]. However, the evidence of chemokine and receptor binding in fish remains limited.…”

Section: Discussionmentioning

confidence: 99%

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The CXC Chemokine Receptors in Four-Eyed Sleeper (Bostrychus sinensis) and Their Involvement in Responding to Skin Injury

Dong

Zhang

et al. 2021

IJMS

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CXC Chemokine signaling plays an important role in wound healing. The four-eyed sleeper (Bostrychus sinensis) is a commercially important marine fish, which is prone to suffer skin ulceration at high temperature seasons, leading to mass mortality of fish in aquaculture farms. The genetic background related to skin ulceration and wound healing has remained unknown in this fish. Herein, we identified 10 differentially expressed Bostrychus sinensis CXC chemokine receptors (BsCXCRs) in skin ulcerated fish by de novo transcriptome sequencing. The transcripts of these BsCXCRs were classified in seven types, including BsCXCR1a/1b, BsCXCR2, BsCXCR3a1/3a2, BsCXCR4a/4b, and BsCXCR5-7, and BsCXCR6 was the first CXCR6 homologue experimentally identified in teleost fish. These BsCXCRs were further characterized in gene and protein structures, as well as phylogenetics, and the results revealed that BsCXCRs have expanded to divergent homologues. Our results showed that, in healthy fish, the BsCXCR transcripts was mainly distributed in the muscle and immune related organs, and that BsCXCR1a/1b proteins located in the cytomembrane, BsCXCR4a/4b/5/6 in the cytomembrane and perinuclear region, and BsCXCR3a1/3a2/7 in the cytomembrane, perinuclear region, and nuclear membrane, respectively. In skin injured fish, the transcripts of all BsCXCRs were transiently increased within one hour after injury, suggesting the involvement of BsCXCRs into the early inflammatory response to skin injury in the four-eyed sleeper. These results are valuable for understanding the evolutionary events of fish CXCR genes and provide insights into the roles of CXCR family in fish skin injury.

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In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus

Dinakaran,

Prasad,

Bedekar

et al. 2024

Arch Virol

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The evolution and functional characterization of CXC chemokines and receptors in lamprey

Cited by 23 publications

References 35 publications

Characterization of Pannexin1, Connexin32, and Connexin43 in Spotted Sea Bass (Lateolabrax maculatus): They Are Important Neuro-Related Immune Response Genes Involved in Inflammation-Induced ATP Release

Characterization of Pannexin1, Connexin32, and Connexin43 in Spotted Sea Bass (Lateolabrax maculatus): They Are Important Neuro-Related Immune Response Genes Involved in Inflammation-Induced ATP Release

The CXC Chemokine Receptors in Four-Eyed Sleeper (Bostrychus sinensis) and Their Involvement in Responding to Skin Injury

In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus

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