The evidence for solid lipid nanoparticles mediated cell uptake of resveratrol

Teskač, Karmen; Kristl, Julijana

doi:10.1016/j.ijpharm.2009.10.011

Cited by 261 publications

(174 citation statements)

References 28 publications

Supporting

Mentioning

158

Contrasting

Unclassified

Order By: Relevance

“…Unfortunately, the extended diffusion of the fluorochrome avoided the colocalization inside the cytoplasm with different organelle labels, thus hampering the determination of the exact localization the nanoparticles inside the cell. These results are consistent with previous uptake studies of LN by Teskac and Kristl [25] that show the rapid internalization and diffuse localization of LN containing 6-coumarin dye in keratinocytes. Nanoparticle uptake was quantified by flow cytometry to obtain a quantitative analysis of NR-LN internalization.…”

Section: 3supporting

confidence: 93%

Edelfosine lipid nanosystems overcome drug resistance in leukemic cell lines

et al. 2013

View full text Add to dashboard Cite

Although current therapies have improved leukemia survival rates, adverse drug effects and relapse are frequent. Encapsulation of edelfosine (ET) in lipid nanoparticles (LN) improves its oral bioavailability and decreases its toxicity. Here we evaluated the efficacy of ET-LN in myeloid leukemia cell lines. Drug-loaded LN were as effective as free ET in sensitive leukemia cell lines. Moreover, the encapsulated drug overcame the resistance of the K562 cell line to the drug. LN containing ET might be used as a promising drug delivery system in leukemia due to their capacity to overcome the in vivo pitfalls of the free drug and their efficacy in vitro in leukemia cell lines.

show abstract

Section: 3supporting

confidence: 93%

Edelfosine lipid nanosystems overcome drug resistance in leukemic cell lines

et al. 2013

View full text Add to dashboard Cite

show abstract

“…As regards solubility characteristics, PEG 400 was added to acceptor medium to maintain sink conditions throughout the testing pro- cedure. Results of biopharmaceutical evaluation revealed that RES was released at a constant speed and the total amount of released RES reached 60.73 % after 6 h. However, the delay of RES appearance in acceptor medium in the initial phase of the experiment could be attributed to the limited diffusion process across the semipermeable membrane and release of RES from ME droplets (5). Results of RES release evaluation demonstrated a constant release rate over the 6 h of testing.…”

Section: Resultsmentioning

confidence: 93%

“…In vitro release studies RES release from MEs was studied by measuring diffusion from a dialysis bag (5). ME-RES samples (1.00 ± 0.02g) were placed into the pre-swelled dialysis bag (Sigma-Aldrich Chemie GmbH, Germany).…”

Section: Evaluation Of Resveratrol Photostabilitymentioning

confidence: 99%

See 1 more Smart Citation

Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stress

2017

View full text Add to dashboard Cite

Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stressResveratrol is well known for its antioxidant activity and susceptibility to ultraviolet radiation. Development of formulations providing improved stability and relevant drug delivery of resveratrol is still a challenging task. The aim of this study was to determine protective characteristics of formulated microemulsions by evaluating photoisomerization of resveratrol and to investigate the effects of resveratrol on human keratinocyte cells under oxidative stress caused by ultraviolet radiation. Incorporation of resveratrol into microemulsions resulted in increased photostability of active compounds and the results demonstrated that photodegradation of resveratrol was significantly delayed. Results of biopharmaceutical evaluation in vitro demonstrated that up to 60 % of resveratrol was released from microemulsions within 6 hours under a constant release rate profile. In vivo biological testing confirmed the ability of resveratrol to protect cells from oxidative stress and to increase cell viability. It was concluded that microemulsions might be considered in the development of UV light sensitive compounds.

show abstract

“…Since they are made from solid lipids, chemically unstable lipid soluble compounds that are sensitive to oxidation can be successfully encapsulated in the SLNs (8, 10). Several molecules were incorporated into lipid nanoparticles, e.g., ascorbyl palmitate, resveratrol, retinol and retinyl palmitate, vitamin E and vitamin E acetate (8,(11)(12)(13)(14). It has been shown that systemic uptake of active substances (e.g., isotretinoin) through skins could be avoided and SLN could increase the accumulative uptake of the active material in the skin (15).…”

mentioning

confidence: 99%

Development and evaluation of coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced dermal delivery

Korkm¹,

Gökçe²,

Özer³

2013

Acta Pharmaceutica

View full text Add to dashboard Cite

Skin is the protective organ of the body with an area around 2 m 2 . The hazardous potential of toxins, pathogens and UV radiation are avoided by the protection that skin supplies (1). UV radiation is the main source of energy that initiates reactive oxygen species (ROS). Formation of ROS leads to oxidative damage in DNA, proteins and lipids. The skin has an enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) and non-enzymatic (antioxidant) protection mechanism against these damages; however, excess ROS production overcomes this protection and causes several diseases such as cancer (2, 3). Hence, effective delivery of an antioxidant into skin in required levels is an important challenge. There are two main steps limiting the effective delivery antioxidants, oxidation of the active substance and carrying the active substance to the viable epidermis.Antioxidants are defined as substances that, when present in a low concentration compared to that of oxidizable substrates, significantly delay or inhibit oxidation of that substance (4). In general, antioxidants act by the chain breaking reaction, by reducing the concentration of reactive oxygen species, by scavenging initiating radicals and by chelating transition metal catalysts (5). Coenzyme Q10 (Q10) is the only lipid soluble endogenous antioxidant. It supports ATP generation during aerobic cellular respiration in the electron transport chain in the mitochondria and inhibits cell membrane peroxidation in reduced form (6, 7). However, it is very difficult to prepare a formulation that helps Q10 delivery to deeper layers of the skin due to the low aqueous solubility of Q10 (log P >10) and due to the barrier function of stratum corneum (8).In the second half of the nineties there was an increasing interest in investigating solid lipid nanoparticles (SLNs) for dermal application. SLNs have many advantages, especially for dermal applications such as occlusion, modulation of drug release and penetration into the skin (9). Since they are made from solid lipids, chemically unstable lipid soluble compounds that are sensitive to oxidation can be successfully encapsulated in the SLNs (8, 10). Several molecules were incorporated into lipid nanoparticles, e.g., ascorbyl palmitate, resveratrol, retinol and retinyl palmitate, vitamin E and vitamin E acetate (8,(11)(12)(13)(14). It has been shown that systemic uptake of active substances (e.g., isotretinoin) through skins could be avoided and SLN could increase the accumulative uptake of the active material in the skin (15). SLNs are produced as liquid dispersions and their incorporation into hydrogels or emulsions helps enhance the retention time in the applied area and patient compliance (16,17).Hydrogels are generally formed from carbomers that have the ability to absorb water, get hydrated and swell by neutralization with a base. Carbomers have been mainly used in liquid or semi-solid pharmaceutical formulations in order to modify their flow characteristics (17,18).The aim of this work was to carry an effic...

show abstract

The evidence for solid lipid nanoparticles mediated cell uptake of resveratrol

Cited by 261 publications

References 28 publications

Edelfosine lipid nanosystems overcome drug resistance in leukemic cell lines

Edelfosine lipid nanosystems overcome drug resistance in leukemic cell lines

Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stress

Development and evaluation of coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced dermal delivery

Contact Info

Product

Resources

About