The evidence for post-meiotic expression of a testis-specific isoform of a regulatory subunit of calcineurin using a monoclonal antibody

Nishio, Hisahide; Matsui, Hideki; Moia, Lizomar J.M.P.; Taketa, Shigeo; Miyamoto, Kazuhiro; Tokuda, Masaaki; Itano, Toshifumi; Nakahara, Sukita; Hatase, Osamu

doi:10.1016/0006-291x(92)91271-q

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…It may be significant that the male germ line contains a unique calcineurin isozyme (Muramatsu et al, 1992;Nishio et al, 1992) that may have its own unique epitopes and inhibitor sensitivities. A possible location for such an enzyme would be the cytoplasmic droplet, which is present on the tails of caput epididymal cells but absent from caudal spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Development of the signalling pathways associated with sperm capacitation during epididymal maturation

Baker

Lewis²,

Hetherington

et al. 2003

Molecular Reproduction Devel

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As spermatozoa mature within the epididymis they acquire the potential for capacitation and ultimately fertilization. In biochemical terms, the former is reflected in the progressive activation of a signal transduction pathway characterized by cAMP-mediated induction of phosphotyrosine expression on the sperm tail. In this study, we have examined the cellular mechanisms controlling this maturational event. Caput epididymal spermatozoa exhibited tyrosine phosphorylation on the sperm head that was largely unresponsive to cAMP and not significantly impaired by removal of extracellular HCO(3) (-). In contrast, caudal epididymal spermatozoa exhibited low levels of phosphorylation on the sperm head, yet responded dramatically to cAMP by phosphorylating a new set of proteins on the sperm tail via mechanisms that were highly dependent on extracellular HCO(3) (-). The impact of extracellular HCO(3) (-) depletion on caudal cells was not associated with a significant change in the redox regulation of cAMP but could be fully reversed by buffering the intracellular pH with N-Tris[Hydroxymethyl]methyl-3-amino-propanesulfonic acid (TAPS). The pattern of tyrosine phosphorylation was also profoundly influenced by the presence or absence of added extracellular calcium. In the presence of this cation, only caudal spermatozoa could respond to increased extracellular cAMP with tyrosine phosphorylation of the sperm tail. However, in calcium-depleted medium, this difference completely disappeared. Under these conditions, caput and caudal spermatozoa were equally competent to exhibit phosphotyrosine expression on the sperm tail in response to cAMP. These results emphasize the pivotal role played by calcium and HCO(3) (-) in modulating the changes in tyrosine phosphorylation observed during epididymal maturation.

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Section: Discussionmentioning

confidence: 99%

Development of the signalling pathways associated with sperm capacitation during epididymal maturation

Baker

Lewis²,

Hetherington

et al. 2003

Molecular Reproduction Devel

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“…Several protein phosphatases are reported to be expressed specifically in the testis [25][26][27][28][45][46][47]. Serine\threonine protein phosphatase PP1γ2 is abundant in the rat testis and localized in the nuclei of late spermatocytes and early spermatids [29].…”

Section: Discussionmentioning

confidence: 99%

“…Spermatogenic cells involved in spermatogenesis are located in the seminiferous tubules, in which a variety of biological events such as proliferation of spermatogonia, meiotic division of spermatocytes and maturation of spermatids to spermatozoa are processed. Although a regulatory mechanism of spermatogenesis is still unclear, protein phosphorylation seems to be involved as an important molecular mechanism in the regulation of spermatogenesis because several protein kinases and phosphatases are expressed specifically in the testis [25][26][27][28][29][30][31][32][33][34]. Recently, it was reported that target disruption of the protein phosphatase PP1γ gene, which encodes an isoform of type-1 serine\threonine protein phosphatase, induces meiotic defect in the testis [35].…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of a novel dual-specificity protein phosphatase possibly involved in spermatogenesis

et al. 1999

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Dual-specificity protein phosphatases (DSPs) play roles in the regulation of mitogenic signal transduction for extracellular stimulation and the cell cycle. In the present study, we identified a novel DSP, termed TMDP (testis- and skeletal-muscle-specific DSP). Nucleotide sequence analysis of TMDP cDNA indicated that the open reading frame of 597 bp encodes a protein of 198 amino acid residues with a predicted molecular mass of 22.5 kDa. The deduced amino acid sequence contains a motif for a conserved catalytic domain of DSPs and shows highest similarity to human Vaccinia HI-related phosphatase (45.5% identity) but low homology to the mitogen-activated protein kinase phosphatase and CDC25 subfamilies of DSPs. Recombinant TMDP protein exhibited intrinsic phosphatase activity towards both phospho-seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Northern-blot analysis revealed that TMDP is most abundantly expressed in the testis. The expression in the testis is characterized as follows: (i) TMDP mRNA first appeared 3 weeks after birth, corresponding to the time that meiosis begins; (ii) TMDP mRNA was abundant in fractionated spermatocytes and round spermatids; and (iii) hybridization in situ showed that the TMDP mRNA is localized in spermatocytes and/or spermatids in seminiferous tubules. These data demonstrate that TMDP is a novel DSP abundantly expressed in the testis and suggest that TMDP may be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis.

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“…In mammalian, testis-specific isoform of CN is expressed when meiosis begins, and increases in amount depending on the maturation of spermatogenesis [40]. In situ hybridization has shown this specific CN isoform was specifically localized to spermatocytes where meiosis occurs, but none or very few have been observed in spermatogonia, spermatids [41].…”

Section: Immunohistochemical Characterization Of Gpca Presence In Nakmentioning

confidence: 99%

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

Wei

Wang²,

Wang³

et al. 2014

Folia Histochem Cytobiol.

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Abstract:The naked carp (Gymnocypris przewalskii), a native teleost, plays an important role in maintenance of the ecological balance in the system of Lake Qinghai (altitude, 3.2 km) on the Qinghai-Tibet Plateau in China. Calcineurin (CN) is the only member of the serine/threonine phosphatase family that can be activated by both Ca 2+ and calmodulin (CaM) and involved in many important physiological processes such as salt tolerance/ /adaption. In this report, cDNAs of CN catalytic subunit paralogue isoforms: GpCAa (GenBank accession no. JQ407043), GpCAg (GenBank accession no. JQ407043), and CN regulatory subunit (GpCB) (GenBank accession no. JQ410473), were isolated from Gymnocypris przewalskii and their expression patterns in embryos development were characterized. Gene expression profile demonstrated that GpCA and GpCB mRNA was distributed ubiquitously in all embryonic stages and showed decline until final stage of development. Immunohistological analysis revealed CN localization in different tissues including kidney, heart, brain, spermary, and gill. Collectively, these results provide molecular basis and clues to further understand the role of CN during embryos development and its function in tissues for the adaptation mechanism of naked carp.

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The evidence for post-meiotic expression of a testis-specific isoform of a regulatory subunit of calcineurin using a monoclonal antibody

Cited by 6 publications

References 12 publications

Development of the signalling pathways associated with sperm capacitation during epididymal maturation

Development of the signalling pathways associated with sperm capacitation during epididymal maturation

Molecular cloning and characterization of a novel dual-specificity protein phosphatase possibly involved in spermatogenesis

Cloning and characterization of two subunits of calcineurin cDNA in naked carp (Gymnocypris przewalskii) from Lake Qinghai, China

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