The ever-expanding diversity and complexity of the
            <i>Arenaviridae</i>
            family

Ly, Hinh

doi:10.1080/21505594.2023.2279353

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…LASV is an enveloped, negative-stranded RNA virus, which contains two single-stranded RNA segments as other mammarenaviruses. However, certain arenaviruses, such as those infecting fish (antennaviruses) and other unknown hosts (innmoviruses), possess three segmented genomes [ 9 , 10 ]. The small (S) and large (L) segments of LASV genome are 3.4 and 7.2 kb in length respectively.…”

Section: Introductionmentioning

confidence: 99%

A novel BSL-2 Lassa virus reverse genetics system modelling the complete viral life cycle

Hu,

Bai,

Tian

et al. 2024

Emerging Microbes & Infections

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Lassa virus (LASV), a risk-group 4 pathogen, must be handled in biosafety level-4 (BSL-4) conditions, thereby limiting its research and antiviral development. Here, we developed a novel LASV reverse genetics system which, to our knowledge, is the first to study the complete LASV life cycle under BSL-2 conditions. Viral particles can be produced efficiently when LASV minigenomic RNA harbouring minimal viral cis -elements and reporter genes is transfected into a helper cell line stably expressing viral NP, GP, Z and L proteins. The resulting defective virions, named LASVmg, can propagate only in the helper cell line, providing a BSL-2 model to study the complete LASV life cycle. Using this model, we found that a previously reported cellular receptor α-dystroglycan is dispensable for LASVmg infection. Furthermore, we showed that ribavirin can inhibit LASVmg infection by inducing viral mutations. This new BSL-2 system should facilitate studying the LASV life cycle and screening antivirals.

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Section: Introductionmentioning

confidence: 99%