The Evaluation of Virus Symptoms and Fruit Quality of Gmo, PPV-Resistant P. Domestica 'Honeysweet' Grown in the Open Field Under a High and Permanent Infection Pressure of Ppv, Aclsv, and PDV

Polak, J.M.; Jarošová, Jana; Kumar, J.; Krška, B.; Gogolková, K.; Sochor, Jiří­; Kizek, René; Scorza, R.; Ravelonandro, M.

doi:10.17660/actahortic.2013.974.7

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The field evaluation (symptoms) and virus detection in C5 has been performed from the very beginning of its introduction in our field experiment. The preliminary results of such evaluation were fragmentally reported earlier (Polák et al 2005(Polák et al , 2013. Here, we have reported complete results of 11 years (2003-2013) investigations of the stability of resistance in C5 to PPV under high infection pressure that has occurred through graft inoculation with PPV-infected budwood from susceptible hosts.…”

Section: Introductionsupporting

confidence: 68%

Transgenic plum Prunus domestica L., clone C5 ( cv. HoneySweet) for protection against sharka disease

Polák

Kundu

Krška

et al. 2017

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Sharka caused by Plum pox virus (PPV) is the most harmful disease of stone fruits in Europe and elsewhere in the world. There is no highly PPV resistant cultivar of plum. Biotech approach has led to the development of resistance through genetic engineering. In this study, we evaluated a transgenic plum Prunus domestica L., clone C5 (cv. HoneySweet), where the PPV resistance is based on RNA interference (RNAi). Resistance in C5 plums has been evaluated for PPV, Prune dwarf virus (PDV), and Apple chlorotic leaf spot virus (ACLSV) in a regulated field trial in the Czech Republic for 12 years (2002-2013). Even under high and permanent infection pressure introduced through graft inoculation of the viruses, PPV has been detected in C5 trees only in several leaves situated close to the point of inoculum grafting in the first nine years. Mild symptoms of PPV disappeared year by year. No PPV symptoms were observed in the last three years and results of ELISA detection were negative. Similar results were obtained, when RT-PCR was used for PPV detection. No natural infection of PPV by aphids was recorded in C5 plums, even if PPV infected plants were growing in the same experimental field in close vicinity. Co-infections of PPV with PDV and/or ACLSV had practically no influence on stability of resistance in C5 trees. Twelve years of field testing in the Czech Republic clearly demonstrated the high level of resistance of C5 either to PPV infection by graft inoculation or natural infection through aphid vectors.

show abstract

Section: Introductionsupporting

confidence: 68%