The Evaluation of Media used to Enumerate Sulphate Reducing Bacteria

Mara, D. Duncan; Williams, David J.

doi:10.1111/j.1365-2672.1970.tb02232.x

Cited by 32 publications

(13 citation statements)

References 15 publications

(8 reference statements)

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“…The technique described here eliminates the need for toxic reducing agents and is simple and rapid. Recovery of pure strains of SRB was comparable to Postgate's medium E (Postgate 1979) and to published recoveries for modified iron sulphite agar (Mara & Williams 1970). The difference between total microscopic and colony counts can be attributed to a proportion of non-viable cells in the enumerated culture (Mara & Williams 1970).…”

Section: Discussionsupporting

confidence: 73%

“…Recovery of pure strains of SRB was comparable to Postgate's medium E (Postgate 1979) and to published recoveries for modified iron sulphite agar (Mara & Williams 1970). The difference between total microscopic and colony counts can be attributed to a proportion of non-viable cells in the enumerated culture (Mara & Williams 1970). The MPN enumeration system is resistant to oxidation through mishandling and the medium was in fact deliberately allowed to oxidize before sample inoculation.…”

Section: Discussionsupporting

confidence: 61%

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A simple most probable number method for the enumeration of sulphate‐reducing bacteria in biocide containing waters

Battersby

Sharma

1985

Journal of Applied Bacteriology

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A simple most probable number (MPN) method has been developed for the enumeration of sulphate-reducing bacteria (SRB) in biocide-containing waters. The medium used is based on source water, it contains no toxic thioglycollate and is resistant to oxidation through mishandling. Reduction is by a suspension of Pseudomonas putida which acts as a powerful adsorbent of biguanide, phenolic, quaternary ammonium compound, glutaraldehyde and isothiazolone biocides. Good recoveries of SRB type strains were obtained using this method and were comparable to other published techniques. Recovery of SRB in mixed culture was comparable to that using a standard laboratory technique.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionsupporting

confidence: 61%

A simple most probable number method for the enumeration of sulphate‐reducing bacteria in biocide containing waters

Battersby

Sharma

1985

Journal of Applied Bacteriology

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“…Cell-free extracts were incubated with ATP and 35SO42as described by Peck (26) and modified by the use of MES buffer (pH 6.8). The complete reaction mixture contained: MES buffer (pH 6.8, 300 Amol: (MgCl2 (20 Atmol); ATP (20 Mmol); Na2SO4, (2.5 gmol [containing 0.1 uCi of 35S-labeled sodium sulfate]); where indicated, Na2MoO4 (2 Mmol); cell-free extract (5.0 mg of protein); total volume, 1.6 ml. Reaction mixtures were incubated for 15 min at 37 C, and the reaction was stopped by being placed in a boiling water bath for 3 min.…”

Section: Appl Microbiolmentioning

confidence: 99%

“…Coleman (8) has suggested that the low numbers of D. ruminis found in the sheep rumen would indicate that this organism is not a significant sulfate-reducing bacterium in ruminants. However, Mara and Williams (20) report that there is no media available yet which I Paper no. 4235 of the Journal series of the North Carolina State University Agricultural Experiment Station.…”

mentioning

confidence: 99%

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1

Huisingh

McNeill

Matrone

1974

Applied Microbiology

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Several dissimilatory, sulfate-reducing bacteria were isolated from the rumen fluid of sheep fed purified diets containing sulfate. One isolate, strain D, was selected for characterization. This organism is a nonsporeforming, obligately anaerobic, mesophilic, nonmotile, gram-negative, straight rod. Cell-free extracts show absorption maxima for cytochrome c3 and desulfoviridin, characteristic of Desulfovibrio. Carbohydrates, as a sole carbon source, will support growth. Lactate supports growth in the presence of sulfate, not in its absence, whereas glucose or pyruvate support growth either in the presence or absence of sulfate. The isolate has a deoxyribonucleic acid base composition of 61.2% guanine plus cytosine, which is similar to that of several other species of Desulfovibrio; however, it differs from previously described species in morphology, motility, and carbon source utilization. Cell-free extracts of this bacterium exhibit adenosine 5'-triphosphate-sulfurylase, adenosine-5'-phosphosulfate-reductase, and hydrogenase activity. After incubation of cell-free extracts with adenine 5'-triphosphate and 3SO042-, adenosine-5'-phosphosulfate rather than 3'-phosphoadenosine-5'-phosphosulfate was shown to be labeled, indicating that the pathway of sulfate reduction in this organism is similar to that of other dissimilatory sulfate reducers. This is the first report of a Desulfovibrio sp. isolated from the rumen.

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“…Flasks were agitated weekly and aliquots of the fluid phase were removed from within the peat-wood chip material. A modified Hungate technique (Hungate, 1969) was used to enumerate the cellulolytic bacteria, whereas the SRB were enumerated with the standard five-tube most probable number (MPN) method (Mara and Williams, 1970). All cultures were incubated at 25° C. An additional control, consisting of peat and the balanced salt solution, was set up to determine whether a viable population of SRB could be established with peat as the sole carbon source.…”

Section: Minmentioning

confidence: 99%

An Evaluation of a Peat-Wood Chip-Microflora Admixture to Act as an Ameliorant for Acid Mine Drainage

Shelp¹,

Southam²,

Spiers³

et al. 1994

ASMR

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Abstract:Peat was investigated as a potential source of indigenous microflora capable of: (!) providing a suitable carbon source for sulfate-reducing bacteria (SRB) through the anaerobic degradation of black spruce (Picea mariana) and trembling aspen (Popu!us tremu!oides) wood chips, (2) complexing metals to form relatively insoluble sulphide minerals through the reduction of sulfate to sulphide, and (3) surviving exposures to extremely acidic conditions. Enumeration studies, combined with measurements of dissolved organic carbon, indicated sufficient available carbon was generated by anaerobic degradation of cellulose to support a viable population of SRB. The bacterial systems showed signs of recovery within 2 to 3 weeks following acidification to pH 3 .0. The reestablishment of active microbial systems was indicated by the formation of a black iron sulphide precipitate.Additional Key Words: acid mine drainage, sulphate reducing bacteria, cellulolytic bacteria, peat, wood waste.

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The Evaluation of Media used to Enumerate Sulphate Reducing Bacteria

Cited by 32 publications

References 15 publications

A simple most probable number method for the enumeration of sulphate‐reducing bacteria in biocide containing waters

A simple most probable number method for the enumeration of sulphate‐reducing bacteria in biocide containing waters

Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen1

An Evaluation of a Peat-Wood Chip-Microflora Admixture to Act as an Ameliorant for Acid Mine Drainage

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