The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells

Hosseini, Seyed Younes; Baesi, Kazem; Azarpira, Negar; Pakneiat, Ameneh; Hosseini, Seyedmohsen

doi:10.3892/br.2017.894

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…These effects are mediated by increased TGF-β secretion in HSC-T6 cells and neutralization of TGF-β can prevent their activation [61]. Unlike the HBV X and HBV C proteins, endogenous expression of HBV pre-core protein does not induce fibrogenic changes in HSCs [62].…”

Section: Hbv-mediated Liver Fibrosismentioning

confidence: 99%

Origin and role of hepatic myofibroblasts in hepatocellular carcinoma

et al. 2020

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Hepatocellular carcinoma (HCC) is the most common primary liver cancer and is the second leading cause of cancer-related death worldwide. Fibrosis and cirrhosis are important risk factors for the development of HCC. Hepatic myofibroblasts are the cells responsible for extracellular matrix deposition, which is the hallmark of liver fibrosis. It is believed that myofibroblasts are predominantly derived from hepatic stellate cells (HSCs), also known as Ito cells. Nevertheless, depending on the nature of insult to the liver, it is thought that myofibroblasts may also originate from a variety of other cell types such as the portal fibroblasts (PFs), fibrocytes, hepatocytes, hepatic progenitor cells (HPCs), and mesothelial cells. Liver myofibroblasts are believed to transform into cancer-associated fibroblasts (CAFs) while HCC is developing. There is substantial evidence suggesting that activated HSCs (aHSCs)/cancer-associated fibroblasts (CAFs) may play an important role in HCC initiation and progression. In this paper, we aim to review current literature on cellular origins of myofibroblasts with a focus on hepatitis B virus (HBV)-and hepatitis C virus (HCV)-induced hepatic fibrosis. We also address the role of aHSCs/CAFs in HCC progression through the regulation of immune cells as well as mechanisms of evolvement of drug resistance.

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Section: Hbv-mediated Liver Fibrosismentioning

confidence: 99%

Origin and role of hepatic myofibroblasts in hepatocellular carcinoma

et al. 2020

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“…A recent study reveals a novel mechanism involving HBc protein in HBV-related hepatocarcinogenesis, indicating that the HBc protein may promote HCC 19 . Conversely, other findings conclude that the precore protein has no significant fibrotic impact 20 .…”

Section: Discussionmentioning

confidence: 99%

A first experience of transduction for differentiated HepaRG cells using lentiviral technology

Pivert

Lefeuvre

Tran

et al. 2019

Sci Rep

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Currently, there is a lack of systems for studying the role of hepatitis B viral proteins, such as HBeAg and HBcAg, on liver injury. It is necessary to develop an original tool in order to clarify the role of these viral proteins in hepatic stellate cell activation, and to understand the molecular mechanisms of liver injury. HepaRG are the most reliable hepatocyte-like cells for studying liver functions or disorders. In this paper, we demonstrate that the transduction of differentiated HepaRG (dHepaRG) cells can be performed successfully using lentiviral particles. The production of a functional Green Fluorescent Protein (GFP) assessed by Fluorescence Activated Cell Sorting and fluorescence microscopy is up to 16% of GFP positive cells using a multiplicity of infection (MOI) of 2.4. We demonstrate that this technology can allow the stable expression of GFP during the long lifecycle of the cell (up to four weeks after the cell’s passage). With this innovative tool, we aim to express viral proteins such as HBeAg or HBcAg in dHepaRG cells. The preliminary results of this work shows that HBeAg can be efficiently produced in dHepaRG cells and that increased MOI allows a better production of this protein. Our future objective will be to study the role of HBc and HBe proteins on the induction of hepatic fibrosis.

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Microcystin-leucine-arginine induces liver fibrosis by activating the Hedgehog pathway in hepatic stellate cells

Yan

Wang

et al. 2020

Biochemical and Biophysical Research Communications

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The evaluation of fibrotic effects of the hepatitis B virus pre-core in hepatic stellate cells

Cited by 5 publications

References 24 publications

Origin and role of hepatic myofibroblasts in hepatocellular carcinoma

Origin and role of hepatic myofibroblasts in hepatocellular carcinoma

A first experience of transduction for differentiated HepaRG cells using lentiviral technology

Microcystin-leucine-arginine induces liver fibrosis by activating the Hedgehog pathway in hepatic stellate cells

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