The Evaluation of Biofilm-Forming Potential of Escherichia coli Collected From the Equine Female Reproductive Tract

Beehan, D.; Wolfsdorf, Karen; Elam, Justine; Krekeler, Natali; Paccamonti, Dale L.; Lyle, Sara K.

doi:10.1016/j.jevs.2015.08.018

Cited by 25 publications

(16 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although undiagnosed infectious endometritis [26][27][28][29][30] may also contribute to unexplained subfertility in mares, presumptive oviductal blockage was considered the most likely cause of unexplained infertility in the mares included in this study. Although undiagnosed infectious endometritis [26][27][28][29][30] may also contribute to unexplained subfertility in mares, presumptive oviductal blockage was considered the most likely cause of unexplained infertility in the mares included in this study.…”

Section: Discussionmentioning

confidence: 99%

“…This study shows that hysteroscopic hydrotubation using saline improved the pregnancy rates in mares which were suspected of oviduct blockage as a cause of the subfertility. Although undiagnosed infectious endometritis [26][27][28][29][30] may also contribute to unexplained subfertility in mares, presumptive oviductal blockage was considered the most likely cause of unexplained infertility in the mares included in this study. The posttreatment achievement of pregnancies in this study therefore, can be attributed to hydrotubation of the oviducts.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Clinical application of hysteroscopic hydrotubation for unexplained infertility in the mare

Inoue

Sekiguchi

2017

Equine Veterinary Journal

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Clinical application of hysteroscopic hydrotubation for unexplained infertility in the mare

Inoue

Sekiguchi

2017

Equine Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…The biofilm formation ability of all the multi-drug resistant isolates was estimated using the crystal violet staining method described by Beehan (Beehan et al, 2015) with minor modifications. An overnight culture of individual isolate grown in tryptic soy broth (TSB) (Oxoid) at 37 o C was adjusted to 0.5 McFarland and were diluted in TSB supplemented with 0.25% glucose (1:40).…”

Section: Biofilm Formationmentioning

confidence: 99%

Detection of enterotoxin gene (sea) and biofilm formation ability among multidrug resistant Staphylococcus aureus isolated from shawarma sandwich sold at selected kiosks in Klang Valley, Malaysia

Fathalla¹,

Mahyudin²,

Ghazali³

et al. 2020

Food Res.

View full text Add to dashboard Cite

The occurrence of multi-drug resistant Staphylococcus aureus in food product of animal origin has increased the concern about their spread into the food supply chain. Presence of multidrug-resistant S. aureus in food products, including ready-to-eat foods imposes potential hazard for consumers. The objective of this research was to investigate the presence of multi-drug resistance of S. aureus in sixty ready-to-eat shawarma sandwiches. Agar-disc diffusion assay determined their resistance to 11 antibiotics. The sea and sed enterotoxin genes were detected by polymerase chain reaction method. Biofilm formation potential (BFP) was quantified by microtitre plate assay. The result revealed that thirty-six samples (60%) were positive for S. aureus. Majority of the isolates (n = 29; 80.6%) were resistant to at least one antibiotic. The isolates demonstrated highest resistance against ampicillin (69.4%) and penicillin (69.4%), while resistance to ciprofloxacin, tetracycline and kanamycin were 47.2%, 33.3% and 22.2%, respectively. Several isolates were resistant to trimethoprim (5.6%), trimethoprim-sulfamethoxazole-(2.8%), gentamicin (2.8%) and cephalothin (2.8%), while none exhibited resistance to chloramphenicol and nitrofurantoin. Out of the thirty-six isolates, twelve isolates (33.3%) were resistant to three or more classes of antibiotic (multidrug-resistant) and 50% had a Multiple Antibiotic Resistance index value more than 0.25. Of the multi-drug resistant isolates, four were positive for sea genes but no sed genes were present. All multi-drug resistance isolates were biofilm formers with five and six isolates were strong and moderate formers, respectively. Additionally, all the sea gene carrying multi-drug resistance isolates were strong biofilm formers. These findings revealed shawarma as a potential vehicle for the spread of multidrug-resistant S. aureus, suggesting more control measures for ready-to-eat food.

show abstract

“…Tissue culture plate assay was used to determine the biofilm formation and according to the procedure mentioned in Beehan et al, Lee et al, and Fattahi et al [41][42][43] with modifications as follows: overnight incubated cultures were diluted to an OD 260 of 0.01 in 5 ml of N.B, LB and AB medium in the six wells TCP (colonies that were grown in LB were inoculated into TCP that contains LB and AB, while colonies that were grown in N.B were inoculated into the TCP that contains N.B) and each strain was cultured in triplicate. All inoculated TCPs were incubated at 37°C in a static incubator (for static cultures) and a shaker incubator at 70 rpm (for the cultures previously incubated in shaker incubator) and at different incubation periods (time points); 6, 12, 24 and 48 h to see at which time point the biofilm form higher [44].…”

Section: Biofilm Formation Assay (Tissue Culture Plate Assay; Tcp)mentioning

confidence: 99%

Adaptability to Various Growth Conditions of Biofilm Associated Extended-Spectrum-Beta-Lactamases Producing Bacteria

Baho¹,

Reid²,

Samarasinghe³

2018

J Infect Dis Diagn

View full text Add to dashboard Cite

Extended-Spectrum β-Lactamase (ESBL) producing bacteria are becoming increasingly prevalent in biofilmassociated infections. Bacteria form biofilms that allow their survival in hostile environments. The amount of formed biofilm is affected by external environmental factors. This study investigates the effect of specific parameters (media type, incubation condition, and growth stage) on the amount of produced biofilm on antibiotic resistant bacterial strains, Escherichia coli (CTX-M-15, TEM-3, and IMP-type) and Klebsiella pneumoniae (OXA-48, SHV-18, NDM-1, and KPC-3). The amount of biofilm formed was measured at different time points (6, 12, 24 and 48 h) of incubations under static and shaking conditions, using three different types of media (nutrient broth, LB broth, and AB broth). Statistical tests showed that there was a significant difference in biofilm level (p<0.01) for 64 out of 80 tests (80%) when grown under different types of media. Growing under different incubation conditions also showed a statistical difference in biofilm level (p<0.05) for 76 out of 120 tests (63%). Stage of growth of the same species also showed statistical difference, 20 out of 24 tests (83%) for E. coli and 24 out of 24 tests (100%) for K. pneumoniae. These findings suggested that biofilm formation is highly affected by incubation conditions, strains' stage of growth, and media type demonstrating that these conditions may play a role in adaptability of the ESBLs on different environmental conditions and their increased prevalence in biofilm associated infections.

show abstract

The Evaluation of Biofilm-Forming Potential of Escherichia coli Collected From the Equine Female Reproductive Tract

Cited by 25 publications

References 17 publications

Clinical application of hysteroscopic hydrotubation for unexplained infertility in the mare

Clinical application of hysteroscopic hydrotubation for unexplained infertility in the mare

Detection of enterotoxin gene (sea) and biofilm formation ability among multidrug resistant Staphylococcus aureus isolated from shawarma sandwich sold at selected kiosks in Klang Valley, Malaysia

Adaptability to Various Growth Conditions of Biofilm Associated Extended-Spectrum-Beta-Lactamases Producing Bacteria

Contact Info

Product

Resources

About