The euryhaline crabUca tangerishowed metabolic differences to sex and environmental salinity

Abstract

This study constitutes a first attempt to investigate sex differences in osmoregulatory capacity and metabolic responses in relation to hyper- and hypo-osmoregulation in the intertidal euryhaline crab Uca tangeri. Adult male and female specimens from Cadiz Bay, Spain (36°23′–37′N 6°8′–15′W), were acclimated to three different environmental salinities (12, 33 and 55 psu) during 7 days, and several parameters were assessed in haemolymph (osmolality, glucose, amino acids, triglycerides and lactate) as well as in metabolic key organs (hepatopancreas, anterior and posterior gills: glycogen, free glucose, amino acids and triglycerides). Specimens from both sex exhibited high and similar hyper- and hypo-osmoregulatory capacities. However, metabolite levels were differentially affected upon acclimation to low and high salinity in several metabolic organs and haemolymph of male and females: (i) glycogen in gills, (ii) free glucose in gills and hepatopancreas, (iii) amino acids in hepatopancreas, (iv) triglycerides in haemolymph, hepatopancreas and posterior gills, and (v) lactate in haemolymph. The results suggest the occurrence of differential metabolic adjustments upon hyper- and hypo-osmoregulation related to sex in the intertidal euryhaline crab U. tangeri.

Effects of low salinity on fatty acid and free amino acid composition of muscle tissues in Portunus trituberculatus

“…Low salinity may greatly affect the fatty acid composition of P. trituberculatus by influencing the osmoregulation mechanism (Ran et al, 2017;Romano et al, 2014;Sarker et al, 2011). There are also evidence show that low salinity can influence the process of physiological metabolism of muscles and other tissues in marine species and then change the nutritional composition of them (Pinoni et al, 2018;Romano et al, 2014). Results showed that in total, 14 kinds of PUFAs and MUFAs were detected in both low salinity group and control group, with total amount accounts for 75.13% and 74.64% in total fatty acid respectively.…”

“…trituberculatus . Several researches have already investigated in other aquatic animals about the impact of low salinity on fatty acid, for example, mud crab Scylla serrata (Romano et al, 2014), mud crab Scylla paramamosain (Wu et al, 2019) and fiddler crab Uca tangeri (Pinoni et al, 2018). However, as little attention was paid to fatty acid composition differences of P .…”

Glucose homeostasis in the euryhaline crab Cytograpsus angulatus : Effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues

“…Changes at molecular and biochemical levels such as those in enzymes and system transports components of the branchial osmoregulatory machine during biochemical adaptation to salinity often requires the mobilization of energy substrates to metabolically deal with salinity challenge (Pinoni et al, 2013;Larsen et al, 2014). This could lead to changes in carbohydrate, lipids, and/or protein metabolism and then to a differential utilization of energy reserves in various storage organs (Pinoni et al, 2013(Pinoni et al, , 2017. The maintenance of glycemia in C. angulatus suggest that availability of glucose from the hemolymph would not be a constraint upon acclimation to different environmental salinities in this crab.…”

“…In estuaries and coastal lagoons, intertidal euryhaline crabs must deal with sharp and broad changes in environmental salinity. Biochemical adaptation to environmental salinity implies adjustments in different tissues (Michiels et al, 2013(Michiels et al, , 2015aPinoni et al, 2013Pinoni et al, , 2017Larsen et al, 2014). In intertidal euryhaline crabs, simultaneous determinations of diverse parameters in different organs are needed for an integral analysis of the adaptation at biochemical level to different salinities (Romano and Zeng, 2012;Pinoni et al, 2013Pinoni et al, , 2015.…”

Effects of Salinity on Muscle Nutrition, Fatty Acid Composition, and Substance Anabolic Metabolism of Blue Tilapia Oreochromis aureus

“…In response to high osmotic stress, O. aureus excretes excess water into the environment, resulting in a lower moisture content in the muscles compared to freshwater species. Tis is in contrast to marine organisms that adapt to low osmotic pressure, such as Uca tangeri crabs, Scylla paramamosain mud crabs, and Sinonovacula constricta [29][30][31]. When fsh experience salinity-induced stress, ash content plays a crucial role in regulating osmotic ions.…”