The Etiology of Anaerobic Infections of Cattle and Comparative Characteristics of the Isolated Strains of Clostridium

Kolesnikova, Yulia; Pimenov, N V; Kapustin, A. V.

doi:10.18551/rjoas.2016-08.07

Cited by 5 publications

(1 citation statement)

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“…Основная сложность реализации задач по разработке новых средств специфической профилактики, заключается в необходимости подбора штаммов микроорганизмов, отвечающих всем требованиям производственных культур. Не смотря на то, что эта часть исследовательской работы занимает достаточно длительный период времени и требует значительных средств и финансовых ресурсов, она является обязательной [5,9,10,13,15].…”

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The Results of the Selection of Mannheimia Haemolytica’s New Strain “Kl-Viev” for the Production of Specific Prevention Agents for Manheimia of Large and Small Cattle

Лаишевцев¹

2017

RJOAS

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Paper presents the results of studying the properties of Mannheimia haemolytica' isolates found in various regions of the Russian Federation during 2015-2017 in terms of serotypic affinity, pathogenicity, virulence and antigenic activity. It was established that two of the five M. haemolytica strains, namely «KL-VIEV», «AM9», №207, №217 and №219, can be used for biopreparation production, since they are suitable for following criteria: «CL-VIEV» and №217 belong to the serotype A1 and virulent for white mice. Based on the obtained data, «KL-VIEV» was selected for industrial usage in the development and manufacture of biopreparations.КЛЮЧЕВЫЕ СЛОВА Биопроизводство, вакцинопрофилактика, пастереллёз, эпизоотический мониторинг, отечественные вакцины, специфическая профилактика, антигенные свойства, методы контроля. KEY WORDS Bioproduction, vaccine, pasteurellosis, epizootic monitoring, domestic vaccine specific prevention, antigenic properties, methods of control.

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The Results of the Selection of Mannheimia Haemolytica’s New Strain “Kl-Viev” for the Production of Specific Prevention Agents for Manheimia of Large and Small Cattle

Лаишевцев¹

2017

RJOAS

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Results of studies on the development of an immuno-enzymed test system for diagnosing infectious enterotoxemia in animals

Spiridonov,

Makhmutov

et al. 2024

BIO Web Conf.

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Research materials on the development of an enzyme-linked immunosorbent assay for the determination of specific antibodies to Cl. perfringens bacteria were presented. Strains of Cl. perfringens were used as production strains: No. 28 (type A), LD-1 (type B), No. 392 (type C), No. 213 (type D). To obtain antigen, a daily suspension of Cl perfringens bacteria, containing 20 billion microbial cells in 1 cm3 (5 billion microbial cells of each serotype), was sounded on an ultrasonic disintegrator at a frequency of 20 MHz for 15 minutes at 4 °C, then the endotoxin was precipitated with ammonium sulfate and purified by differential dissolution of the precipitate in 0.02 M phosphate buffer, followed by dialysis against tap water. Control positive serum with specific antibodies of at least 1:12800 to antigens of Cl. perfringens bacteria was obtained by hyperimmunization of cattle with corpuscular antigens and toxoids. The basic conditions of the ELISA reaction were standardized. Determination of the concentration of sorption of the antigen was carried out by parallel testing of various dilutions of the conjugate. The most acceptable titer of the blood serum of animals was found at a dilution of the conjugate of 1:2500 and a concentration of the sorbed antigen of 5-8 μg/cm3. The production test of the enzyme immunoassay test system was carried out in cattle farms in three regions of the Russian Federation, unfavorable for anaerobic enterotoxemia in calves.

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Clinical trial results of an associated vaccine against cattle clostridiosis and escherichiosis

Spiridonov,

Makhmutov

et al. 2024

BIO Web Conf.

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In veterinary practice, gastrointestinal diseases of newborn calves, caused by the association of pathogenic bacteria, in particular Clostridium perfringens and Escherichia coli, are often observed. The results of our research have shown that in the Volga Federal District of the Russian Federation, anaerobic enterotoxemia in newborn calves is most often caused by Clostridium perfringens serotypes A, C, D. For the specific prevention of this form of pathology, an associated vaccine has been developed containing inactivated antigens of Clostridium perfringens serotypes A, C, D and Escherichia coli, which produce adhesion factors K99 and A20. Clinical trials of the effectiveness of the vaccine on cattle were carried out in 3 large agricultural enterprises of the Republic of Tatarstan for the production of milk, permanently dysfunctional for clostridiosis and escherichiosis of newborn calves. Disease prevention began with the immunization of pregnant cows and heifers 60 days before the expected calving. The animals were vaccinated twice with an interval of 14 days at a dose of 10 cm3. Calves obtained from immunized cows were vaccinated twice subcutaneously at a dose of 3 cm3 at the age of 18- 20 days. A total of 3156 cows and heifers were vaccinated in three dairy complexes. It was found that the associated vaccine is harmless for deepwalled cows and heifers, does not cause post-vaccination complications. Immunization of animals in the last months of pregnancy contributes to the accumulation of specific antibodies in colostrum, the intake of which provides passive protection of the newborn calf from infection with Cl. perfringens and E. coli bacteria.

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The Etiology of Anaerobic Infections of Cattle and Comparative Characteristics of the Isolated Strains of Clostridium

Cited by 5 publications

References 0 publications

The Results of the Selection of Mannheimia Haemolytica’s New Strain “Kl-Viev” for the Production of Specific Prevention Agents for Manheimia of Large and Small Cattle

The Results of the Selection of Mannheimia Haemolytica’s New Strain “Kl-Viev” for the Production of Specific Prevention Agents for Manheimia of Large and Small Cattle

Results of studies on the development of an immuno-enzymed test system for diagnosing infectious enterotoxemia in animals

Clinical trial results of an associated vaccine against cattle clostridiosis and escherichiosis

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