The ethylene responsive factor CdERF1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance

Hu, Zhengrong; Huang, Xin; Amombo, Erick; Liu, Ao; Fan, Jibiao; Bi, Aoyue; Ji, Kang; Xin, H. P.; Chen, Liang; Fu, Jinmin

doi:10.1016/j.plantsci.2020.110432

Cited by 44 publications

(25 citation statements)

References 53 publications

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“…These results were highly correlated with the variations of FPKM values in the organ-specific transcriptome dataset since the Pearson correlation R value of the two results were all higher than 0.85 ( Supplementary Table 7 ). Similar expression quantification result of ERF1 was also obtained in a study analyzing the function of ERF1 in cold tolerance of bermudagrass ( Hu et al, 2020 ), which further verified the correctness of our results.…”

Section: Resultssupporting

confidence: 89%

Organ-Specific Transcriptome Analysis Identifies Candidate Genes Involved in the Stem Specialization of Bermudagrass (Cynodon dactylon L.)

Chen

et al. 2021

Front. Genet.

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As an important warm-season turfgrass and forage grass species with wide applications, bermudagrass (Cynodon dactylon L.) simultaneously has shoot, stolon and rhizome, three types of stems with different physiological functions. To better understand how the three types of stems differentiate and specialize, we generated an organ-specific transcriptome dataset of bermudagrass encompassing 114,169 unigenes, among which 100,878 and 65,901 could be assigned to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Ontology (GO) terms, respectively. Using the dataset, we comprehensively analyzed the gene expression of different organs, especially the shoot, stolon and rhizome. The results indicated that six organs of bermudagrass all contained more than 52,000 significantly expressed unigenes, however, only 3,028 unigenes were enrich-expressed in different organs. Paired comparison analyses further indicated that 11,762 unigenes were differentially expressed in the three types of stems. Gene enrichment analysis revealed that 39 KEGG pathways were enriched with the differentially expressed unigenes (DEGs). Specifically, 401 DEGs were involved in plant hormone signal transduction, whereas 1,978 DEGs were transcription factors involved in gene expression regulation. Furthermore, in agreement with the starch content and starch synthase assay results, DEGs encoding starch synthesis-related enzymes all showed the highest expression level in the rhizome. These results not only provided new insights into the specialization of stems in bermudagrass but also made solid foundation for future gene functional studies in this important grass species and other stoloniferous/rhizomatous plants.

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Section: Resultssupporting

confidence: 89%

Organ-Specific Transcriptome Analysis Identifies Candidate Genes Involved in the Stem Specialization of Bermudagrass (Cynodon dactylon L.)

Chen

et al. 2021

Front. Genet.

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“…Additionally, the BRs-regulated bHLH transcription factors CESTA and BZR1 stimulate the transcription of CBFs genes, which activate the expression of cold responsive genes (Eremina et al 2016;Li et al 2017). Consistent with the ndings of these studies, our data showed that exogenous melatonin upregulated the expression of DREB1A, ERF1, ERF109, MYB108, MYB4, WRKY30, WRKY53, bZIP73, ZAT6, and MADS57 and several genes that encod dehydrin (Supplementary Table S3); these genes have been reported to be involved in abiotic stress, including cold and drought stress (Shi et al 2015;Xu et al 2019;Liu et al 2019;Wang et al 2019;Hu et al 2020). These results suggest that melatonin and BRs function together in response to cold and drought stress, in which melatonin induces the accumulation of BRs and subsequently activates a wide range of stress-responsive genes.…”

Section: Brassinosteroid Biosynthesis and Its Signaling Pathway Are Induced By Melatoninsupporting

confidence: 89%

Melatonin-induced cold and drought tolerance requires brassinosteroids and hydrogen peroxide signaling in perennial ryegrass

Fu¹,

Zhang²,

Jiang³

et al. 2021

Preprint

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Aims The interplay between melatonin and brassinosteroids in enhancing tolerance to cold and drought and the underlying molecular mechanisms of this relationship still remain unknown. Methods Combined physiological and transcriptomic analyses were used to clarify the crosstalk of melatonin and brassinosteroids in perennial ryegrass. Brassinosteroids biosynthesis inhibitor propiconazole, Arabidopsis brassinosteroids-receptor mutant bri1-9, bak1, and H2O2 deficient rbohC and rbohF mutants were used to analyzed the role of H2O2 in melatonin and brassinosteroids-mediated tolerance to the stress. Results Melatonin-enhanced cold and drought tolerance of perennial ryegrass depends on the duration of dose and stress that were applied. Melatonin activates the expression of NADPH oxidase-related genes to promote H2O2 production when exposed to short-term cold/drought stresses but reduced accumulation of H2O2 under long-term stress. This was associated with an increase in enzymatic and non-enzymatic antioxidant activities by regulation of their gene expression and modulation of the ascorbate-glutathione cycle. Moreover, exogenous melatonin increased the biosynthesis of melatonin and brassinosteroids or the expression of signaling-related genes. However, the effects of melatonin on the expression of BR biosynthesis and signaling-related genes were inhibited in the rbohC and rbohF mutants. Chemical scavenging of H2O2 attenuated melatonin-mediated growth in the Arabidopsis wild-type, but the bak1 and bri1.9 mutants were relatively insensitive. Consistently, Inhibition of H2O2 production impaired the effect of melatonin and brassinosteroids on seed germination and the root growth of perennial ryegrass. Conclusions This research reveals a novel regulatory mechanism of the crosstalk of H2O2 and brassinosteroid signaling in melatonin-induced cold/drought tolerance in perennial ryegrass.

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“…Similar findings have been reported in bermudagrass; CdERF1 improved cold tolerance by regulating stress-associated gene expression levels, relieving reactive oxygen species (ROS) damage, and improving the antioxidant system [30]. These earlier findings suggest that cold tolerance of a plant is particularly dependent on ethylene.…”

Section: Introductionsupporting

confidence: 76%

Overexpression of MdARD4 Accelerates Fruit Ripening and Increases Cold Hardiness in Tomato

Guo

Zhang

et al. 2020

IJMS

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Ethylene plays an important role in stress adaptation and fruit ripening. Acireductone dioxygenase (ARD) is pivotal for ethylene biosynthesis. However, the response of ARD to fruit ripening or cold stress is still unclear. In this study, we identified three members of Malus ARD family, and expression profile analysis revealed that the transcript level of MdARD4 was induced during apple fruit ripening and after apple plants were being treated with cold stress. To investigate its function in cold tolerance and fruit ripening, MdARD4 was ectopically expressed in Solanum lycopersicum cultivar ‘Micro-Tom’, which has been considered as an excellent model plant for the study of fruit ripening. At the cellular level, the MdARD protein expressed throughout Nicotiana benthamiana epidermal cells. Overexpression of MdARD4 in tomato demonstrated that MdARD4 regulates the ethylene and carotenoid signaling pathway, increases ethylene and carotenoid concentrations, and accelerates fruit ripening. Furthermore, MdARD4 increased the antioxidative ability and cold hardiness in tomato. To conclude, MdARD4 may potentially be used in apple breeding to accelerate fruit ripening and increase cold hardiness.

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The ethylene responsive factor CdERF1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance

Cited by 44 publications

References 53 publications

Organ-Specific Transcriptome Analysis Identifies Candidate Genes Involved in the Stem Specialization of Bermudagrass (Cynodon dactylon L.)

Organ-Specific Transcriptome Analysis Identifies Candidate Genes Involved in the Stem Specialization of Bermudagrass (Cynodon dactylon L.)

Melatonin-induced cold and drought tolerance requires brassinosteroids and hydrogen peroxide signaling in perennial ryegrass

Overexpression of MdARD4 Accelerates Fruit Ripening and Increases Cold Hardiness in Tomato

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