The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco

Zhang, Haiwen; Huang, Zejun; Xie, Bingyan; Chen, Qi; Tian, Xin; Zhang, Xiulin; Zhang, Hongbo; Lu, Xiangyang; Huang, Dafang; Huang, Rongfeng

doi:10.1007/s00425-004-1347-x

Cited by 209 publications

(164 citation statements)

References 55 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…How frequent such complex regulations are, remains to be seen. There are however several parallels to our findings, e.g., the highly complex network regulating Arabidopsis response to Botrytis infection, 28 the JERF1 transcription factor in tomato, which is induced by NaCl, ethylene, jasmonate and ABA 29 or the integration of stress responses via the DELLA proteins. 30 It seems therefore, that such complex signaling networks might be a rule rather than exception, especially for processes regulated by stress.…”

supporting

confidence: 76%

Lessons from investigation of regulation of APS reductase by salt stress

Kopřivová

2008

Plant Signaling & Behavior

View full text Add to dashboard Cite

Exposure to high salinity affects plant ion homeostasis, water relations and results in oxidative stress. Therefore, various processes are induced as salt stress response including antioxidative defense systems. The tripeptide glutathione has a prominent position among the metabolites involved in such stress defense. Glutathione synthesis is dependent on supply of cysteine and thus on the assimilation of sulfate. We have investigated how the key enzyme of sulfate assimilation, adenosine 5'phosphosulfate (APS) reductase is regulated by salt stress in Arabidopsis roots. Using Arabidopsis mutants in various signaling pathways we aimed to identify the signaling cascade leading to regulation of APS reductase by NaCl. We found the enzyme to be regulated by a complex signaling network on transcriptional and post-transcriptional levels with responses of mRNA accumulation and enzyme activity largely uncoupled. Here we want to share the important lessons we have learned from this investigations.Sulfur metabolism plays an important role in plant stress response. 1,2 Among the sulfur containing metabolites, the tripeptide glutathione (GSH) is the most important part of this response due to its function in the ascorbate glutathione cycle in detoxification of reactive oxygen species (ROS). 3 As salt stress is connected with ionic stress, osmotic stress and production of ROS it is not surprising that it also triggers increase of GSH levels. 4-6 GSH synthesis is dependent on the availability of the constituent amino acids cysteine, glutamate and glycine and as such, on sufficient reduction of sulfate. 7 The key control point of sulfate assimilation is the reduction of activated sulfate, APS, to sulfite by APS reductase. 8 Accordingly, APS reductase is highly regulated in a demand driven manner, i.e., its activity increases when concentration of reduced sulfur compounds is low, e.g., during sulfur starvation or depletion of GSH, and decreases when excess reduced sulfur is available. [8][9][10] In vast majority of reports, changes in APS reductase activity correlated well with changes in mRNA and protein accumulation, indicating a simple transcriptional regulation of the corresponding genes, 8-10 with a rare occurrence of additional level of post-transcriptional redox regulation. 11 Whereas the physiological responses of APS reductase to different environmental stimuli are well known, very little is known about the molecular mechanisms including signaling pathways and transcription factors.To find components of its regulatory circuit we chose salt stress as the environmental stimuli and analyzed the regulation of APS reductase in roots of Arabidopsis mutants in different signaling pathways. 6 In that report we showed that in roots of wild-type (WT) Arabidopsis, APS reductase activity, protein accumulation, and mRNA levels were increased 3-fold after 5 hours of exposure to 150 mM NaCl. Analysis of various mutants in hormone signaling revealed that the regulation was ABA insensitive, however, otherwise the response of APS reductase...

show abstract

supporting

confidence: 76%

Lessons from investigation of regulation of APS reductase by salt stress

Kopřivová

2008

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…As shown in Table I, several enzymes involved in the synthesis of ethylene or polyamines (SAM synthetase, ACC oxidase, and Arg decarboxylase) and jasmonic acid (12-oxophytodienoate reductase) are indeed expressed in the exocarp, and at least two proteins, EREBP-3 (Leubner-Metzger et al, 1998) and JERF1 (Zhang et al, 2004), are ethyleneresponsive transcription factors that may participate in the regulation of stress tolerance and fruit growth (Jones et al, 2002;Balbi and Lomax, 2003). In addition, a wide range of regulatory proteins can modulate gene expression in the exocarp from growing fruit (Table I).…”

Section: Regulation Of Exocarp-expressed Genesmentioning

confidence: 99%

Changes in Transcriptional Profiles Are Associated with Early Fruit Tissue Specialization in Tomato

et al. 2005

View full text Add to dashboard Cite

The cell expansion phase contributes in determining the major characteristics of a fleshy fruit and represents two-thirds of the total fruit development in tomato (Solanum lycopersicum). So far, it has received very little attention. To evaluate the interest of a genomic scale approach, we performed an initial sequencing of approximately 1,200 cell expansion stage-related sequence tags from tomato fruit at 8, 12, and 15 d post anthesis. Interestingly, up to approximately 35% of the expressed sequence tags showed no homology with available tomato expressed sequence tags and up to approximately 21% with any known gene. Microarrays spotted with expansion phase-related cDNAs and other fruit cDNAs involved in various developmental processes were used (1) to profile gene expression in developing fruit and other plant organs and (2) to compare two growing fruit tissues engaged mostly in cell division (exocarp) or in cell expansion (locular tissue surrounding the seeds). Reverse transcription-polymerase chain reaction analysis was further used to confirm microarray results and to specify expression profiles of selected genes (24) in various tissues from expanding fruit. The wide range of genes expressed in the exocarp is consistent with a protective function and with a high metabolic activity of this tissue. In addition, our data show that the expansion of locular cells is concomitant with the expression of genes controlling water flow, organic acid synthesis, sugar storage, and photosynthesis and suggest that hormones (auxin and gibberellin) regulate this process. The data presented provide a basis for tissue-specific analyses of gene function in growing tomato fruit.After ovule fertilization, early tomato (Solanum lycopersicum) fruit development is characterized by a period of cell division followed by cell expansion, resulting in the formation of large vacuolated cells (Mohr and Stein, 1969). Cell expansion is the longest phase in fruit development and may contribute to 90% of the increase in fruit weight, depending on the cultivar. In addition, cell expansion contributes, along with ripening, to the major structural, biochemical, and physiological changes that characterize a fleshy fruit. The cell wall changes associated with cell enlargement and the continuous accumulation in the vacuole of water, sugars, organic acids, and other compounds are necessary to maintain the turgor pressure of the expanding cells. In addition, these physiological processes contribute significantly to the flavor, texture, and overall attractiveness of the ripe fruit. The succession of the different phases of tomato fruit development is not, however, as clear cut as described above. It varies greatly according to the fruit tissue considered (Joubès et al., 1999). Tomato fruit is a complex organ composed of two or more carpels separated by a radially orientated tissue called septum, which results from the fusion of two adjacent carpel walls (or pericarp). The pericarp encloses the locular cavity containing the seeds attached to a central pa...

show abstract

“…The plants were developed at 25°C under a long-day photoperiod. Various treatments were performed on 2-week-old plants as described previously [9,10,16].…”

Section: Plant Materials and Stress Treatmentsmentioning

confidence: 99%

The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box

et al. 2006

View full text Add to dashboard Cite

Four AP2/EREBP genes encoding putative ethyleneresponsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/ AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.

show abstract

The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco

Cited by 209 publications

References 55 publications

Lessons from investigation of regulation of APS reductase by salt stress

Lessons from investigation of regulation of APS reductase by salt stress

Changes in Transcriptional Profiles Are Associated with Early Fruit Tissue Specialization in Tomato

The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box

Contact Info

Product

Resources

About