The Ethyl Acetate Subfraction of &lt;i&gt;Polygonum cuspidatum&lt;/i&gt; Root Containing Emodin Affect EBV Gene Expression and Induce EBV-Positive Cells Apoptosis

Yiu, Ching-Yi; Chiu, Yu-Jhe; Lin, Tsuey-Pin

doi:10.1248/bpb.b21-00508

Cited by 6 publications

(8 citation statements)

References 29 publications

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“…Additionally, as the phosphorylation of I‐κBα in the cytoplasm can promote its own degradation, its degradation decreases. This leads to increased binding of I‐κBα to NF‐κB, resulting in NF‐κB functional inactivation and induction of cell apoptosis 23 . These findings highlight the molecular mechanisms through which F3a exerts its anti‐tumour effects.…”

Section: Latent Membrane Proteinsmentioning

confidence: 88%

“…Polygonum cuspidatum, a traditional Chinese herbal medicine, has a long history of use in treating various diseases, such as atherosclerosis, arthritis, asthma, hyperlipidaemia, and cancer. 22,23 Recent studies have shown that F3a and resveratrol present in the extract can induce cell death in EBVassociated tumour cells. 24 F3a stimulates the production of LMP1 and prevents intracellular phosphorylation of ERK and I-κBα, which in turn reduces cell proliferation and promotes apoptosis.…”

Section: Drugs Targeting Lmp1 Through Affecting the Nf-κb Pathwaymentioning

confidence: 99%

“…24 F3a stimulates the production of LMP1 and prevents intracellular phosphorylation of ERK and I-κBα, which in turn reduces cell proliferation and promotes apoptosis. 23 These effects have significant therapeutic ramifications for EBV-related malignancies. On the one hand, F3a promotes the expression of LMP1.…”

Section: Drugs Targeting Lmp1 Through Affecting the Nf-κb Pathwaymentioning

confidence: 99%

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Targeting EBV‐encoded products: Implications for drug development in EBV‐associated diseases

Lv,

Ding,

Zhang

et al. 2023

Reviews in Medical Virology

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Epstein‐Barr virus, a human gamma‐herpesvirus, has a close connection to the pathogenesis of cancers and other diseases, which are a burden for public health worldwide. So far, several drugs or biomolecules have been discovered that can target EBV‐encoded products for treatment, such as Silvestrol, affinity toxin, roscovitine, H20, H31, curcumin, thymoquinone, and ribosomal protein L22. These drugs activate or inhibit the function of some biomolecules, affecting subsequent signalling pathways by acting on the products of EBV. These drugs usually target LMP1, LMP2; EBNA1, EBNA2, EBNA3; EBER1, EBER2; Bam‐HI A rightward transcript and BHRF1. Additionally, some promising findings in the fields of vaccines, immunological, and cellular therapies have been established. In this review, we mainly summarise the function of drugs mentioned above and unique mechanisms, hoping that we can help giving insight to the design of drugs for the treatment of EBV‐associated diseases.

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Section: Latent Membrane Proteinsmentioning

confidence: 88%

Section: Drugs Targeting Lmp1 Through Affecting the Nf-κb Pathwaymentioning

confidence: 99%

Section: Drugs Targeting Lmp1 Through Affecting the Nf-κb Pathwaymentioning

confidence: 99%

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Targeting EBV‐encoded products: Implications for drug development in EBV‐associated diseases

Lv,

Ding,

Zhang

et al. 2023

Reviews in Medical Virology

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“…In addition, found that the cell proliferation of Burkitt’s lymphoma-derived Raji cells, which are EBV-positive cells, could be suppressed by the Polygonum cuspidatum ethyl acetate subfraction containing emodin (F3a) via increasing the intracellular reactive-oxygen species (ROS), activating the apoptosis-related proteins, and increasing the apoptosis percentage Yiu et al (2021) . These results mean that emodin may be a therapeutic drug for EBV-related tumors.…”

Section: Antiviral Activity Of Emodinmentioning

confidence: 99%

Promising Role of Emodin as Therapeutics to Against Viral Infections

et al. 2022

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Emodin is an anthraquinone derivative that is widely present in natural plants and has a wide spectrum of pharmacological effects, such as antibacterial, anti-inflammatory, anti-fibrotic and anticancer and so on. Through reviewing studies on antiviral effect of emodin in the past decades, we found that emodin exhibits ability of inhibiting the infection and replication of more than 10 viruses in vitro and in vivo, including herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), coxsackievirus B (CVB), hepatitis B virus (HBV), influenza A virus (IAV), SARS-CoV, viral haemorrhagic septicaemia rhabdovirus (VHSV), enterovirus 71 (EV71), dengue virus serotype 2 (DENV-2) and Zika virus (ZIKV). Therefore, this review aims to summarize the antiviral effect of emodin, in order to provide reference and hopes to support the further investigations.

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“…Earlier studies exhibit that the ethanolic extract of P. cuspidatum and resveratrol inhibit EBV lytic cycle and induce EBV-positive cell apoptosis (Yiu et al, 2010;Yiu et al, 2014;Wu et al, 2018;Yiu et al, 2021). The ethanolic extract of P. cuspidatum root contains anthraglycoside B, physcion, piceid, emodin, and resveratrol (Yun-Ting et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Chemical Components of Polygonum cuspidatum Ethylacetate Subfraction and their Effects on Epstein-Barr Virus Lytic Genes Expression

Yiu¹,

Kuan²,

Chen³

et al. 2022

J Biochem Technol

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The study highlights the effect of ethylacetate subfraction F1 from Polygonum cuspidatum root and piceid on the inhibition of Epstein-Barr virus (EBV) lytic gene expression. The MTT [3-(4,5dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method was employed to assess the effect of F1 and piceid on the cell survival of P3HR1. The immunoblotting method and flow cytometry were applied to analyze the expression of EBV lytic proteins. The results showed that the EC50 (effective concentration required to inhibit 50% EBV lytic proteins) of F1 and piceid inhibiting the lytic proteins of EBV were 23.3 μg/mL and 119.6 μM, respectively. For P3HR1 cytotoxicity, the CC50 (cytotoxic concentration that decreased cell viability by 50%) of F1 and piceid were 93.1 μg/mL and 507.9 μM, respectively. In other words, F1 and piceid effectively inhibited the expression of EBV lytic proteins at a non-cytotoxic concentration. qPCR was performed to analyze the effect of F1 and piceid on the transcription of lytic genes, including BRLF1, BZLF1, and DNA replication of EBV. The results showed that F1 and piceid inhibited the transcription of lytic genes and reduced DNA replication of EBV with EC50 of 55.5 μg/mL and 89.9 μM, respectively. The results of this study confirmed that F1 and piceid could effectively inhibit the expression of EBV lytic proteins and EBV DNA replication, indicating that F1 and piceid are potentials for use as anti-EBV drugs.

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The Ethyl Acetate Subfraction of <i>Polygonum cuspidatum</i> Root Containing Emodin Affect EBV Gene Expression and Induce EBV-Positive Cells Apoptosis

Cited by 6 publications

References 29 publications

Targeting EBV‐encoded products: Implications for drug development in EBV‐associated diseases

Targeting EBV‐encoded products: Implications for drug development in EBV‐associated diseases

Promising Role of Emodin as Therapeutics to Against Viral Infections

Chemical Components of Polygonum cuspidatum Ethylacetate Subfraction and their Effects on Epstein-Barr Virus Lytic Genes Expression

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